| Size | Price | |
|---|---|---|
| Other Sizes |
| ln Vitro |
In vitro, Cinnamamide exhibits low cytotoxicity against BEL-7402 human hepatoma cells and HT-1080 fibrosarcoma cells and inhibits cell growth with IC50 values of 1.94 and 1.29 mM, respectively. It inhibits the production of ROS. It acts on matrix metalloproteinase.
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|---|---|
| ln Vivo |
In vivo, Cinnamamide reduced tumor weight in a mouse model of C26 murine colon carcinoma. It has been shown to regulate skin cell proliferation. It is a useful standard for analytical and bioassay dereplication of crude microbial extracts.
|
| Enzyme Assay |
In vitro assays for Cinnamamide typically measure its cytotoxicity and antitumor activity. Cell viability is assessed using MTT or CellTiter-Glo assays in cancer cell lines. Its effects on matrix metalloproteinase activity can be assessed using zymography or fluorogenic substrates.
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| Cell Assay |
Cellular assays for Cinnamamide are performed using cancer cell lines (e.g., BEL-7402, HT-1080). Cells are treated with the compound, and cell viability is assessed. ROS production is measured using fluorescent probes such as DCFH-DA.
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| Animal Protocol |
In vivo animal studies with Cinnamamide are conducted in mouse models of colon carcinoma. Mice bearing tumors are treated with the compound. Tumor weight is measured. Its efficacy in inhibiting tumor growth is evaluated.
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| ADME/Pharmacokinetics |
Cinnamamide is an amide of trans-cinnamic acid. Its molecular weight is 147.17 g/mol. For research use, it is typically dissolved in DMSO for in vitro studies. Its half-life and metabolic profile have been characterized.
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| Toxicity/Toxicokinetics |
Cinnamamide exhibits low cytotoxicity. No significant toxicity has been reported at the doses used for efficacy studies.
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| Additional Infomation |
Cinnamamide is the simplest member of the cinnamamide class, consisting of an acrylamide molecule with a phenyl substituent attached at the 3-position. It has been reported to exist in plants of the Kaempferiaceae family (Aristolochia kaempferi), Reseda luteola, and other organisms with relevant data. See also: 2-acrylamide, 3-phenyl- (note moved to).
Cinnamamide is a natural product and a metabolite of Streptomyces. It is an antitumor agent with low cytotoxicity. The compound has been shown to reduce tumor weight in a mouse model of colon carcinoma. Cinnamamide is not approved for clinical use and is intended for laboratory research only. |
| Molecular Formula |
C9H9NO
|
|---|---|
| Molecular Weight |
147.1739
|
| Exact Mass |
147.068
|
| CAS # |
621-79-4
|
| PubChem CID |
5273472
|
| Appearance |
Off-white to yellow solid powder
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
350.3±25.0 °C at 760 mmHg
|
| Melting Point |
148-150 °C(lit.)
|
| Flash Point |
165.6±23.2 °C
|
| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.614
|
| LogP |
1.35
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
11
|
| Complexity |
157
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=C(/C(/[H])=C(\[H])/C1C([H])=C([H])C([H])=C([H])C=1[H])N([H])[H]
|
| InChi Key |
APEJMQOBVMLION-VOTSOKGWSA-N
|
| InChi Code |
InChI=1S/C9H9NO/c10-9(11)7-6-8-4-2-1-3-5-8/h1-7H,(H2,10,11)/b7-6+
|
| Chemical Name |
(E)-3-phenylprop-2-enamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~679.5 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (16.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7949 mL | 33.9743 mL | 67.9486 mL | |
| 5 mM | 1.3590 mL | 6.7949 mL | 13.5897 mL | |
| 10 mM | 0.6795 mL | 3.3974 mL | 6.7949 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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