| Size | Price | |
|---|---|---|
| Other Sizes |
| ln Vitro |
In vitro, Cinnamamide exhibits low cytotoxicity against BEL-7402 human hepatoma cells and HT-1080 fibrosarcoma cells and inhibits cell growth with IC50 values of 1.94 and 1.29 mM, respectively. It inhibits the production of ROS. It acts on matrix metalloproteinase.
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|---|---|
| ln Vivo |
In vivo, Cinnamamide reduced tumor weight in a mouse model of C26 murine colon carcinoma. It has been shown to regulate skin cell proliferation. It is a useful standard for analytical and bioassay dereplication of crude microbial extracts.
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| Enzyme Assay |
In vitro assays for Cinnamamide typically measure its cytotoxicity and antitumor activity. Cell viability is assessed using MTT or CellTiter-Glo assays in cancer cell lines. Its effects on matrix metalloproteinase activity can be assessed using zymography or fluorogenic substrates.
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| Cell Assay |
Cellular assays for Cinnamamide are performed using cancer cell lines (e.g., BEL-7402, HT-1080). Cells are treated with the compound, and cell viability is assessed. ROS production is measured using fluorescent probes such as DCFH-DA.
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| Animal Protocol |
In vivo animal studies with Cinnamamide are conducted in mouse models of colon carcinoma. Mice bearing tumors are treated with the compound. Tumor weight is measured. Its efficacy in inhibiting tumor growth is evaluated.
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| ADME/Pharmacokinetics |
Cinnamamide is an amide of trans-cinnamic acid. Its molecular weight is 147.17 g/mol. For research use, it is typically dissolved in DMSO for in vitro studies. Its half-life and metabolic profile have been characterized.
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| Toxicity/Toxicokinetics |
Cinnamamide exhibits low cytotoxicity. No significant toxicity has been reported at the doses used for efficacy studies.
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| Additional Infomation |
Cinnamamide is the simplest member of the cinnamamide class, consisting of an acrylamide molecule with a phenyl substituent attached at the 3-position. It has been reported to exist in plants of the Kaempferiaceae family (Aristolochia kaempferi), Reseda luteola, and other organisms with relevant data. See also: 2-acrylamide, 3-phenyl- (note moved to).
Cinnamamide is a natural product and a metabolite of Streptomyces. It is an antitumor agent with low cytotoxicity. The compound has been shown to reduce tumor weight in a mouse model of colon carcinoma. Cinnamamide is not approved for clinical use and is intended for laboratory research only. |
| Molecular Formula |
C9H9NO
|
|---|---|
| Molecular Weight |
147.1739
|
| Exact Mass |
147.068
|
| CAS # |
621-79-4
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| PubChem CID |
5273472
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| Appearance |
Off-white to yellow solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
350.3±25.0 °C at 760 mmHg
|
| Melting Point |
148-150 °C(lit.)
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| Flash Point |
165.6±23.2 °C
|
| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.614
|
| LogP |
1.35
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
11
|
| Complexity |
157
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=C(/C(/[H])=C(\[H])/C1C([H])=C([H])C([H])=C([H])C=1[H])N([H])[H]
|
| InChi Key |
APEJMQOBVMLION-VOTSOKGWSA-N
|
| InChi Code |
InChI=1S/C9H9NO/c10-9(11)7-6-8-4-2-1-3-5-8/h1-7H,(H2,10,11)/b7-6+
|
| Chemical Name |
(E)-3-phenylprop-2-enamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7949 mL | 33.9743 mL | 67.9486 mL | |
| 5 mM | 1.3590 mL | 6.7949 mL | 13.5897 mL | |
| 10 mM | 0.6795 mL | 3.3974 mL | 6.7949 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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