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| Other Sizes |
| Targets |
As a synthetic dye, C.I. Orange G does not have a specific pharmacological target. Its primary applications are as a histological stain and industrial dye. The compound is used to stain erythrocytes in trichrome methods when combined with other yellow dyes in alcoholic solution. It may interact with biological tissues through non-specific binding.
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| ln Vitro |
C.I. Orange G itself is not known to possess direct therapeutic activity. Its significance is as a histological stain and industrial dye. The compound is used for faint background staining and as a counterstain for various nuclear stains. It is also used to stain erythrocytes in trichrome methods. There is no known therapeutic activity.
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| ln Vivo |
There is no reported in vivo therapeutic activity for C.I. Orange G. The compound is primarily used as a histological stain and industrial dye. It is used in non-textile industrial applications including dyeing paper, staining wood, and coloring inks and copying pencils. Its role in biological systems is as a staining agent rather than a pharmacologically active compound.
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| Enzyme Assay |
As a synthetic dye, there are no standardized in vitro enzyme/receptor binding assays for C.I. Orange G. The compound's utility is as a histological stain and industrial dye. Researchers may study the compound's staining properties and interactions with biological tissues in the context of histology and microscopy.
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| Cell Assay |
There are no reported in vitro cellular assays for C.I. Orange G as a therapeutic agent. The compound is used as a histological stain. Cellular studies may be conducted to assess the compound's staining properties, toxicity, or metabolism, but these are not therapeutic assays.
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| Animal Protocol |
There are no reported in vivo animal studies for C.I. Orange G as a therapeutic agent. The compound is primarily used as a histological stain and industrial dye. Animal studies may be conducted to assess the compound's toxicity or staining properties, but these are not therapeutic studies.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
Rabbits fed 500 mg/kg body weight of orange juice excreted 40% in urine as p-aminophenol, 3% as o-aminophenol, and 0.6% as free aniline… Rats given a single oral dose of 250 mg/kg body weight of orange juice excreted 61% in urine as p-aminophenol, while aniline was detected in feces. No altered dye was detected in urine or feces… In humans, after administration of 20 mg/kg body weight of Orange G, 95% of the urine consisted of p-aminophenol, 0.5% of aniline, and 1.3% of the unchanged pigment… Bacteria in rat feces and rat liver homogenate can reduce the azo bonds of Orange G… As a synthetic dye used in histology and industrial applications, there are limited pharmacokinetic data for C.I. Orange G. The compound has a molecular weight of 452.36 and is an orange microcrystalline powder. It dyes wool and silk directly from formic acid or sulfuric acid baths. Further pharmacokinetic data is not available. |
| Toxicity/Toxicokinetics |
The toxicological properties of C.I. Orange G have not been fully investigated. As an azo dye, it may be metabolized to aromatic amines. The compound should be handled with appropriate safety precautions. Toxicological studies would be needed to fully characterize its safety profile for any potential therapeutic or industrial applications.
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| Additional Infomation |
Ci (Citral Orange) 10 is an orange microcrystalline or powdery substance. (NTP, 1992)
Orange G is an organosodium salt, the disodium salt of 7-hydroxy-8-[(E)-phenyldiazonyl]naphthalene-1,3-disulfonic acid. It is often mixed with other yellow dyes in alcoholic solutions for staining erythrocytes in trichrome staining, and is also used to visualize pancreatic and pituitary cells. It is a histological dye. Its structure contains 7-hydroxy-8-[(E)-phenyldiazonyl]naphthalene-1,3-disulfonic acid salt. C.I. Orange G (Acid Orange 10, C.I. 16230) is a synthetic azo dye used primarily as a histological stain for faint background staining and as a counterstain for various nuclear stains including hematoxylin, safranin O, crystal violet, methyl green, and basic fuchsin. It is also used to stain erythrocytes in trichrome methods and in various industrial applications including dyeing paper, staining wood, and coloring inks. |
| Molecular Formula |
C16H10N2NA2O7S2
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|---|---|
| Molecular Weight |
452.36
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| Exact Mass |
451.972
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| CAS # |
1936-15-8
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| PubChem CID |
16015
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| Appearance |
Orange to red solid powder
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| Density |
0.80 g/mL at 20 °C
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| Melting Point |
141 °C
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| Flash Point |
14 °C
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| LogP |
4.93
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
29
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| Complexity |
724
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
HSXUHWZMNJHFRV-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2
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| Chemical Name |
disodium;7-hydroxy-8-phenyldiazenylnaphthalene-1,3-disulfonate
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| Synonyms |
NSC 10450; C.I. 16230; C.I. Orange G
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2106 mL | 11.0531 mL | 22.1063 mL | |
| 5 mM | 0.4421 mL | 2.2106 mL | 4.4213 mL | |
| 10 mM | 0.2211 mL | 1.1053 mL | 2.2106 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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