| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| 100mg | |||
| Other Sizes |
| Targets |
C.I. 37575 does not have a specific biological target in the context of pharmacology. As a dye, its function is to serve as a chromogenic substrate for enzymes such as acid and alkaline phosphatase. When the enzyme cleaves the dye, it produces a colored precipitate at the site of enzyme activity, allowing for the visualization of enzyme localization and activity in histological samples.
|
|---|---|
| ln Vitro |
In vitro, C.I. 37575 is used as a histochemical substrate. Its "activity" is its ability to be cleaved by phosphatases, producing a colored product that can be visualized microscopically. It is not a drug and has no direct biological activity in the sense of modulating a signaling pathway. Its use is confined to diagnostic and research applications in histology and pathology.
|
| ln Vivo |
C.I. 37575 is not used in vivo as a drug. It is a chemical reagent for in vitro applications. Its use is strictly for research and diagnostic purposes, such as staining tissue sections to detect enzyme activity. It is not administered to living organisms for therapeutic or experimental purposes.
|
| Enzyme Assay |
The experimental protocol for C.I. 37575 involves its use as a substrate in histochemical staining. In a typical procedure, tissue sections are incubated in a solution containing the dye and a suitable buffer. If the target enzyme (e.g., alkaline phosphatase) is present, it cleaves the dye, releasing a colored product that precipitates at the site of enzyme activity. The sections are then counterstained and examined under a microscope.
|
| Cell Assay |
C.I. 37575 is not used in cell-based assays in the same way as a drug. Its use is in histochemistry, where it is applied to fixed tissue sections to detect enzyme activity. It can be used in cytological preparations, but its primary application is in tissue sections. The protocol involves incubating the sample with the dye and then visualizing the resulting colored precipitate.
|
| Animal Protocol |
C.I. 37575 is not used in animal experiments as a therapeutic or experimental agent. Its use is confined to in vitro applications in histology and pathology. It is not administered to animals, as its purpose is to stain tissue sections for the detection of enzyme activity.
|
| ADME/Pharmacokinetics |
C.I. 37575 has a molecular weight of 584.62 and a molecular formula of C₃₆H₂₈N₂O₆. It is a solid powder that should be stored away from direct sunlight. It can be stored as a powder at -20°C for up to 3 years or in solution at -80°C for 1 year. It is shipped with blue ice.
|
| Toxicity/Toxicokinetics |
C.I. 37575 is a dye and is not intended for therapeutic use. Its toxicity is not a primary concern in a laboratory setting, but standard chemical safety precautions should be taken. It is handled as a laboratory reagent, and appropriate personal protective equipment (e.g., gloves, lab coat) should be worn to avoid skin contact and inhalation.
|
| References | |
| Additional Infomation |
C.I. 37575, also known as Naphthol AS-BR, is a dye used as a substrate for acid and alkaline phosphatase in histochemistry. It is a chemical reagent with the molecular formula C₃₆H₂₈N₂O₆ and a molecular weight of 584.62. It is not a drug and has no clinical applications. Its primary use is in research and diagnostic laboratories for staining tissue sections to detect enzyme activity.
|
| Molecular Formula |
C36H28N2O6
|
|---|---|
| Molecular Weight |
584.61732
|
| Exact Mass |
584.195
|
| CAS # |
91-92-9
|
| PubChem CID |
7068
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.373g/cm3
|
| Boiling Point |
701.2ºC at 760 mmHg
|
| Flash Point |
377.9ºC
|
| Index of Refraction |
1.746
|
| LogP |
7.739
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
44
|
| Complexity |
902
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
AXUHYNGMYQMRRI-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C36H28N2O6/c1-43-33-19-25(11-13-29(33)37-35(41)27-15-21-7-3-5-9-23(21)17-31(27)39)26-12-14-30(34(20-26)44-2)38-36(42)28-16-22-8-4-6-10-24(22)18-32(28)40/h3-20,39-40H,1-2H3,(H,37,41)(H,38,42)
|
| Chemical Name |
3-hydroxy-N-[4-[4-[(3-hydroxynaphthalene-2-carbonyl)amino]-3-methoxyphenyl]-2-methoxyphenyl]naphthalene-2-carboxamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~8.55 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7105 mL | 8.5526 mL | 17.1051 mL | |
| 5 mM | 0.3421 mL | 1.7105 mL | 3.4210 mL | |
| 10 mM | 0.1711 mL | 0.8553 mL | 1.7105 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
