| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Chlorbicyclen targets the nervous system of insects as a cyclodiene insecticide. Like other cyclodienes, it acts as a non-competitive antagonist at the GABA-gated chloride channel in the insect nervous system, blocking inhibitory neurotransmission and causing hyperexcitation, convulsions, and death. It has high lipophilicity and bioaccumulation potential.
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|---|---|
| ln Vitro |
In vitro studies have evaluated chlorbicyclen's insecticidal activity against various insect species. It shows potent activity against target agricultural pests. However, specific IC₅₀/EC₅₀ values for insect cell lines or enzyme targets are not extensively documented in the available literature.
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| ln Vivo |
In vivo, chlorbicyclen exhibits insecticidal efficacy in agricultural field applications. It is effective against a range of insect pests. Due to its organochlorine nature, it has environmental persistence and bioaccumulation concerns. Specific animal model data for mammalian toxicity are limited.
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| Enzyme Assay |
In vitro enzyme assays may be used to evaluate GABA receptor chloride channel inhibition. Membrane preparations from insect nervous tissue are incubated with [³H]-EBOB or [³H]-TBOB to assess binding displacement. Electrophysiological studies using oocyte-expressed insect GABA receptors can evaluate functional antagonism.
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| Cell Assay |
Insect cell lines (e.g., Sf9, High Five) or primary neuronal cultures can be used to assess cytotoxicity and GABA receptor function. Cells are treated with chlorbicyclen at various concentrations, and cell viability is measured by MTT or ATP-based assays. Electrophysiological recordings (patch clamp) can assess chloride current inhibition.
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| Animal Protocol |
In vivo insecticidal efficacy is evaluated in agricultural field trials or laboratory assays using target pest species (e.g., beetles, caterpillars). Insects are exposed to chlorbicyclen via contact or ingestion at various concentrations, and mortality is recorded over 24-72 hours. LC₅₀ values are calculated from dose-response curves.
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| ADME/Pharmacokinetics |
As an organochlorine pesticide, chlorbicyclen has high lipophilicity (LogP ~5.54), extensive tissue distribution, and slow elimination. It accumulates in adipose tissue and undergoes minimal metabolism. Environmental persistence is a major concern. In mammals, it is slowly excreted, primarily in feces.
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| Toxicity/Toxicokinetics |
Chlorbicyclen exhibits significant toxicity to non-target organisms including mammals, birds, and aquatic life. Like other cyclodienes, it is neurotoxic and can cause convulsions. Chronic exposure may lead to liver and kidney damage. Due to environmental persistence and toxicity, its use has been restricted or banned in many countries.
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| Additional Infomation |
Chlorobicycloene is an organochlorine compound.
Chlorbicyclen (Alodan) is an organochlorine cyclodiene insecticide with IUPAC name 1,2,3,4,7,7-hexachloro-5,6-bis(chloromethyl)bicyclo[2.2.1]hept-2-ene. It is a persistent organic pollutant and has been largely withdrawn from agricultural use due to environmental and health concerns. It remains available as a research standard for environmental analysis. |
| Molecular Formula |
C9H6CL8
|
|---|---|
| Molecular Weight |
397.75
|
| Exact Mass |
393.798
|
| CAS # |
2550-75-6
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| PubChem CID |
17357
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| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.74g/cm3
|
| Boiling Point |
410.6ºC at 760mmHg
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| Melting Point |
104-106°
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| Flash Point |
202.9ºC
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| Vapour Pressure |
1.41E-06mmHg at 25°C
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| Index of Refraction |
1.598
|
| LogP |
5.541
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
0
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| Rotatable Bond Count |
2
|
| Heavy Atom Count |
17
|
| Complexity |
357
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
ClCC1C(CCl)C2(Cl)C(=C(Cl)C1(C2(Cl)Cl)Cl)Cl
|
| InChi Key |
FUZORIOHZSVKAW-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H6Cl8/c10-1-3-4(2-11)8(15)6(13)5(12)7(3,14)9(8,16)17/h3-4H,1-2H2
|
| Chemical Name |
1,2,3,4,7,7-hexachloro-5,6-bis(chloromethyl)bicyclo[2.2.1]hept-2-ene
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| Synonyms |
AC 12402; Nodon; Chlorbicyclen
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5141 mL | 12.5707 mL | 25.1414 mL | |
| 5 mM | 0.5028 mL | 2.5141 mL | 5.0283 mL | |
| 10 mM | 0.2514 mL | 1.2571 mL | 2.5141 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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