| Size | Price | Stock | Qty |
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| 5g |
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| Other Sizes |
| Targets |
Celestine Blue does not have a specific biological target in the traditional pharmacological sense. Its mechanism of action is chemical and relates to its staining properties. Celestine Blue forms a complex with iron ions (Fe³⁺), and this iron-Celestine Blue complex binds to nucleic acids, particularly DNA, through electrostatic and intercalative interactions. The blue-black color of the complex allows for the visualization of nuclei and chromatin in histological sections. The compound does not have pharmacological activity; its utility is as a histochemical stain.
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| ln Vitro |
Celestine Blue is used as a histochemical stain for the demonstration of nucleic acids. When complexed with iron, Celestine Blue stains nuclei and chromatin a blue-black color. The staining is selective for nucleic acids and can be used to highlight nuclear morphology in histological sections. Celestine Blue is often used in combination with other stains, such as hematoxylin, for routine histological staining. The compound does not exhibit pharmacological activity.
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| ln Vivo |
Celestine Blue does not have in vivo pharmacological activity. It is a histochemical stain used for research and diagnostic applications, primarily in vitro and ex vivo, for the staining of tissue sections and cytological preparations. The compound is not administered to animals for therapeutic or pharmacological studies. Its use is limited to histological and pathological applications.
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| Enzyme Assay |
Celestine Blue is not used in non-cellular enzyme/receptor binding assays. Its application is limited to histological staining. The staining procedure involves preparing tissue sections on glass slides, deparaffinizing and rehydrating the sections, and incubating them with the Celestine Blue-iron complex staining solution. The stained sections are then rinsed, dehydrated, cleared, and mounted for microscopic examination.
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| Cell Assay |
In vitro cellular assays for Celestine Blue are not typically conducted for pharmacological evaluation. The compound is used as a histochemical stain for fixed cells and tissues. Cells grown on coverslips or tissue sections are fixed, and the Celestine Blue-iron complex is applied to stain the nuclei. The stained cells or tissues are examined by light microscopy.
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| Animal Protocol |
Celestine Blue is not used in in vivo animal studies as a therapeutic or pharmacological agent. Its application is limited to ex vivo staining of tissue sections and cytological preparations collected from animals in research studies. The staining procedure is the same as that used for human samples and allows for the visualization of nuclear morphology in animal tissues.
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| ADME/Pharmacokinetics |
Celestine Blue is not a drug and does not have pharmacokinetic properties. Its physical and chemical properties are more relevant: it is a synthetic oxazine dye with a molecular weight of 361.78 and a molecular formula of C₁₇H₁₆ClN₃O₄. The compound is soluble in water and alcohol and is typically prepared as an aqueous or alcoholic solution for use as a stain.
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| Toxicity/Toxicokinetics |
Celestine Blue is considered a hazardous chemical. It may cause skin, eye, and respiratory tract irritation. The compound is toxic if swallowed. Celestine Blue is a potential carcinogen and should be handled with appropriate precautions. Gloves, goggles, and protective clothing should be worn when handling the compound. Work should be conducted in a well-ventilated area or in a fume hood. The compound should be disposed of in accordance with local regulations for hazardous waste.
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| References |
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| Additional Infomation |
Celestin blue B is an organochlorine salt composed of 1-carbamoyl-7-(diethylamino)-3,4-dihydroxyphenoxazine-5-onium and chloride ions in a 1:1 ratio. It is a histological dye, often used in combination with iron alum mordant as a substitute for hematoxylin in hematoxylin-eosin (H&E) staining. It can be used as both a fluorescent dye and a histological dye. It contains Celestin blue B(1+).
Celestine Blue is a research-grade histochemical stain intended for laboratory use only and is not approved for clinical use. Its CAS number is 1562-90-9. The primary applications of Celestine Blue include the staining of nuclei and chromatin in histological sections and cytological preparations, counterstaining in histopathology, and as a component of staining protocols for the demonstration of nucleic acids. The compound is often used in combination with hematoxylin for nuclear staining in routine histology. Celestine Blue is a valuable tool for histology, pathology, and biomedical research. |
| Molecular Formula |
C17H18N3O4+.CL-
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|---|---|
| Molecular Weight |
363.79552
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| Exact Mass |
363.098
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| CAS # |
1562-90-9
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| PubChem CID |
135403650
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| Appearance |
Brown to black solid powder
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| Boiling Point |
431.7ºC at 760mmHg
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| Melting Point |
227-230 °C
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| Flash Point |
214.9ºC
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| LogP |
0.322
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
25
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| Complexity |
648
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
REPMZEQSQQAHJR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H17N3O4.ClH/c1-3-20(4-2)9-5-6-11-13(7-9)24-16-14(19-11)10(17(18)23)8-12(21)15(16)22;/h5-8,19H,3-4H2,1-2H3,(H2,18,23);1H
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| Chemical Name |
[7-(diethylamino)-3,4-dioxo-10H-phenoxazine-1-carbonyl]azanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7488 mL | 13.7438 mL | 27.4876 mL | |
| 5 mM | 0.5498 mL | 2.7488 mL | 5.4975 mL | |
| 10 mM | 0.2749 mL | 1.3744 mL | 2.7488 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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