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| Other Sizes |
| Targets |
CAPSO does not have a specific biological target. It is a zwitterionic buffer used to maintain pH in biochemical and biological experiments. Good's buffers are characterized by high water solubility, low cell membrane permeability, consistent acid-base dissociation constants, low metal chelating capability, and high chemical stability. CAPSO is used in various applications including diagnostic assay manufacturing and as a buffering agent in biological and biochemical research.
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| ln Vitro |
CAPSO does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for biochemical and biological research. The compound maintains a stable pH environment for experiments without interfering with the biochemical processes being studied. It is used in buffer solutions for various in vitro applications including enzyme assays and protein purification.
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| ln Vivo |
CAPSO does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro and ex vivo applications.
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| Enzyme Assay |
In vitro biochemical assays using CAPSO buffer are performed to maintain pH during experiments. The buffer is prepared at the desired pH within its effective range (8.9-10.3). CAPSO was used as a biological buffer to maintain pH 9 during water leach tests for the analysis of high-carbon coal fly ash-soil mixtures. It is also used in chemiluminescent in situ hybridization and preparation of planar bilayer lipid membranes.
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| Cell Assay |
Cell culture experiments using CAPSO are limited as its pH range (8.9-10.3) is above physiological pH, making it unsuitable for most cell culture applications. It may be used in cell lysis buffers or in protein extraction protocols at alkaline pH. Researchers should consult primary literature for specific applications.
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| Animal Protocol |
In vivo animal experiments are not applicable to CAPSO as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to CAPSO as it is a buffer reagent rather than a therapeutic agent. The compound has a molecular weight of 237.32, a molecular formula of C9H19NO4S, a pKa of 9.6, and a useful pH range of 8.9-10.3. Solubility: water: 0.111 g/mL. Appearance: crystalline powder. Storage: room temperature.
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| Toxicity/Toxicokinetics |
CAPSO is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. Good's buffers are designed for minimal interference with biochemical processes. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use.
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| Additional Infomation |
3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid is an amine.
CAPSO has CAS number 73463-39-5, molecular formula C9H19NO4S, and molecular weight 237.32. Synonyms: 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid. It is a zwitterionic Good's buffer with a pH range of 8.9-10.3 and a pKa of 9.6. Purity: ≥99%. Not for human use; for research purposes only. |
| Molecular Formula |
C9H19NO4S
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|---|---|
| Molecular Weight |
237.3165
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| Exact Mass |
237.103
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| CAS # |
73463-39-5
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| PubChem CID |
2733480
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Melting Point |
270-274°C
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| Index of Refraction |
1.538
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| LogP |
-0.36
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
15
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| Complexity |
266
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C([H])([H])C([H])(C([H])([H])N([H])C1([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C1([H])[H])O[H])(=O)(=O)O[H]
|
| InChi Key |
INEWUCPYEUEQTN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H19NO4S/c11-9(7-15(12,13)14)6-10-8-4-2-1-3-5-8/h8-11H,1-7H2,(H,12,13,14)
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| Chemical Name |
3-(cyclohexylamino)-2-hydroxypropane-1-sulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2137 mL | 21.0686 mL | 42.1372 mL | |
| 5 mM | 0.8427 mL | 4.2137 mL | 8.4274 mL | |
| 10 mM | 0.4214 mL | 2.1069 mL | 4.2137 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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