| Size | Price | Stock | Qty |
|---|---|---|---|
| 500μg |
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| 1mg | |||
| Other Sizes |
| Targets |
Campestanol does not have a specific molecular target in the same way as a drug. Its primary biological effect is related to its ability to lower serum cholesterol levels. It is thought to compete with cholesterol for absorption in the intestine, thereby reducing the amount of cholesterol that enters the bloodstream. This mechanism is shared by other plant sterols and stanols.
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|---|---|
| ln Vitro |
In vitro, Campestanol is used as a standard or reference compound in studies of phytosterol metabolism and cholesterol absorption. It is not a drug with a typical "activity" in cell-based assays. Its effects are measured in intestinal cell models, where it can inhibit cholesterol uptake. It is also used as an analytical standard for the identification and quantification of phytosterols in food and biological samples.
|
| ln Vivo |
In vivo, Campestanol, when administered as part of a diet, has been shown to lower serum cholesterol and LDL levels. Dietary administration of vegetable stanol esters containing campestanol and sitostanol has been shown to be effective in reducing cholesterol levels. This effect is the basis for its use as a functional food ingredient for cholesterol management.
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| Enzyme Assay |
Campestanol is a phytosterol and is typically studied in the context of cholesterol metabolism. In vitro assays often involve using Caco-2 cells, a model of intestinal absorption. In these assays, cells are treated with Campestanol, and its ability to inhibit the uptake of [¹⁴C]-cholesterol is measured. This provides a measure of its cholesterol-lowering potential.
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| Cell Assay |
Cellular assays for Campestanol involve studying its effects on cholesterol uptake and metabolism in intestinal cells. In a typical protocol, Caco-2 cells are grown on transwell inserts to form a polarized monolayer. Campestanol is added to the apical compartment, and its ability to inhibit the transport of cholesterol from the apical to the basolateral compartment is measured.
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| Animal Protocol |
The in vivo efficacy of Campestanol is evaluated in animal models or human intervention studies. In these studies, subjects are fed a diet supplemented with Campestanol or plant stanol esters. The primary endpoint is the change in serum total cholesterol and LDL-cholesterol levels. These studies confirm its cholesterol-lowering effect.
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| ADME/Pharmacokinetics |
Campestanol has a molecular weight of 400.68 and a molecular formula of C₂₈H₅₀O. It is a naturally occurring phytosterol found in various plant sources. It is structurally similar to cholesterol and is often used as a dietary supplement or functional food ingredient for cholesterol management. Its physicochemical properties are well-characterized.
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| Toxicity/Toxicokinetics |
Campestanol is a phytosterol and is generally recognized as safe (GRAS) for use as a food ingredient. It is not considered toxic at the levels typically consumed in the diet. Its safety has been evaluated in numerous studies, and it is widely used as a functional food ingredient for lowering cholesterol.
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| References | |
| Additional Infomation |
Campesterol is a 3β-sterol belonging to the C28 steroid family. It is derived from the hydride of 5α-campesterane. Campesterol has been reported to be found in tung oil trees, maize, and other organisms with relevant data. See also: Ergosterol (note moved here).
Campestanol is a naturally occurring phytosterol found in vegetables, fruits, grains, and nuts. It is structurally similar to cholesterol and has been shown to lower serum cholesterol and LDL levels. It is used as a dietary supplement or functional food ingredient for cholesterol management. It is not a drug and has no clinical applications as a therapeutic agent. |
| Molecular Formula |
C28H50O
|
|---|---|
| Molecular Weight |
402.71
|
| Exact Mass |
402.386
|
| CAS # |
474-60-2
|
| PubChem CID |
119394
|
| Appearance |
White to off-white solid powder
|
| Density |
0.951g/cm3
|
| Boiling Point |
461.4ºC at 760 mmHg
|
| Flash Point |
193ºC
|
| Vapour Pressure |
1.87E-10mmHg at 25°C
|
| Index of Refraction |
1.502
|
| LogP |
7.714
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
29
|
| Complexity |
569
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
O([H])[C@@]1([H])C([H])([H])C([H])([H])[C@@]2(C([H])([H])[H])[C@]([H])(C1([H])[H])C([H])([H])C([H])([H])[C@]1([H])[C@]2([H])C([H])([H])C([H])([H])[C@]2(C([H])([H])[H])[C@@]([H])([C@]([H])(C([H])([H])[H])C([H])([H])C([H])([H])[C@@]([H])(C([H])([H])[H])C([H])(C([H])([H])[H])C([H])([H])[H])C([H])([H])C([H])([H])[C@]21[H]
|
| InChi Key |
ARYTXMNEANMLMU-ATEDBJNTSA-N
|
| InChi Code |
InChI=1S/C28H50O/c1-18(2)19(3)7-8-20(4)24-11-12-25-23-10-9-21-17-22(29)13-15-27(21,5)26(23)14-16-28(24,25)6/h18-26,29H,7-17H2,1-6H3/t19-,20-,21+,22+,23+,24-,25+,26+,27+,28-/m1/s1
|
| Chemical Name |
(3S,5S,8R,9S,10S,13R,14S,17R)-17-[(2R,5R)-5,6-dimethylheptan-2-yl]-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol
|
| Synonyms |
Campestanol Ergostanol
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4832 mL | 12.4159 mL | 24.8318 mL | |
| 5 mM | 0.4966 mL | 2.4832 mL | 4.9664 mL | |
| 10 mM | 0.2483 mL | 1.2416 mL | 2.4832 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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