| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
Callistephin chloride does not have a well-defined single therapeutic target but exhibits multiple biological activities. As an anthocyanin, it acts as an antioxidant by scavenging free radicals and reducing oxidative stress. The compound has been examined for its neuroprotective properties. It is also used as a natural colorant in food and research applications. The compound's antioxidant activity contributes to its potential health benefits.
|
|---|---|
| ln Vitro |
Callistephin chloride exhibits antioxidant activity by scavenging free radicals and reducing oxidative stress. The compound has been examined for its neuroprotective properties. As an anthocyanin, it is a flavonoid with potential health benefits. Callistephin chloride is a natural colorant found in strawberries and other fruits and flowers. It is used in research to study the bioactivity of anthocyanins and their potential applications in health and nutrition.
|
| ln Vivo |
In vivo activity data for callistephin chloride are limited but suggest potential health benefits. The compound has been examined for its neuroprotective properties, which may be relevant for the prevention or treatment of neurodegenerative diseases. As an antioxidant, it may help reduce oxidative stress in vivo. However, specific in vivo efficacy studies are not extensively documented. The compound is primarily studied for its bioactivity as a natural anthocyanin.
|
| Enzyme Assay |
In vitro antioxidant assays for callistephin chloride typically involve DPPH (2,2-diphenyl-1-picrylhydrazyl) or ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging assays. The compound is dissolved in appropriate solvents (ethanol or DMSO) at varying concentrations and incubated with the radical solution. The decrease in absorbance is measured spectrophotometrically to determine the IC50 value for radical scavenging. The compound's antioxidant activity is compared to standard antioxidants such as ascorbic acid or Trolox.
|
| Cell Assay |
In vitro cellular assays for callistephin chloride typically involve treating cultured cells (such as neuronal cells or other cell types) with the compound at concentrations ranging from 1 to 100 μM for 24-72 hours. Cellular viability is assessed using MTT or resazurin reduction assays. Neuroprotective effects are evaluated by challenging cells with oxidative stress-inducing agents (such as hydrogen peroxide or glutamate) and measuring cell survival. The compound is dissolved in DMSO as a stock solution and diluted in cell culture medium.
|
| Animal Protocol |
In vivo animal studies for callistephin chloride are not extensively documented. For potential in vivo applications, the compound could be administered to mice or rats via oral gavage or dietary supplementation at doses determined from preliminary studies. Neuroprotective effects could be evaluated in models of neurodegenerative diseases. Oxidative stress markers and antioxidant enzyme activities could be measured in blood and tissues. However, specific protocols for in vivo studies are not well-established in the public domain.
|
| ADME/Pharmacokinetics |
Callistephin chloride has a molecular weight of 468.84 g/mol and formula C21H21ClO10. The compound is soluble in methanol and other organic solvents. It is typically stored desiccated at -20°C. Detailed PK parameters such as half-life, Cmax, AUC, and bioavailability would require experimental determination. As an anthocyanin, it is expected to have limited oral bioavailability due to its polar nature.
|
| Toxicity/Toxicokinetics |
Toxicity data for callistephin chloride are not extensively reported. As a naturally occurring anthocyanin found in edible berries, it is generally considered to have low toxicity. The compound is intended for research use only and is not approved for human therapeutic applications. Standard toxicity assessments would include acute toxicity, genotoxicity, and repeated-dose toxicity studies. Appropriate safety precautions should be taken when handling.
|
| References | |
| Additional Infomation |
Geraniol-3-O-β-D-glucoside chloride is a member of the anthocyanin chloride family, and its cationic counterpart is geraniol-3-O-β-D-glucoside. It contains geraniol-3-O-β-D-glucoside.
Callistephin chloride (CAS 18466-51-8) is a naturally occurring anthocyanin found in strawberries, chokeberries, and other berries. It has a molecular weight of 468.84 g/mol and formula C21H21ClO10. The compound is also known as pelargonidin 3-O-glucoside chloride. Callistephin chloride exhibits antioxidant activity and is examined for neuroprotective properties. It is used as a natural colorant and in research on anthocyanin bioactivity. The compound is not approved for clinical use. |
| Molecular Formula |
C21H21CLO10
|
|---|---|
| Molecular Weight |
468.8384
|
| Exact Mass |
468.082
|
| CAS # |
18466-51-8
|
| Related CAS # |
Callistephin chloride; 18466-51-8
|
| PubChem CID |
3080714
|
| Appearance |
Light brown to brown solid powder
|
| Source |
Originated from plants: Rosaceae Fragaria × ananassa Duch.
|
| Hydrogen Bond Donor Count |
7
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
32
|
| Complexity |
585
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
C1=CC(=CC=C1C2=[O+]C3=CC(=CC(=C3C=C2O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O)O)O.[Cl-]
|
| InChi Key |
CAHGSEFWVUVGGL-UBNZBFALSA-N
|
| InChi Code |
InChI=1S/C21H20O10.ClH/c22-8-16-17(26)18(27)19(28)21(31-16)30-15-7-12-13(25)5-11(24)6-14(12)29-20(15)9-1-3-10(23)4-2-9;/h1-7,16-19,21-22,26-28H,8H2,(H2-,23,24,25);1H/t16-,17-,18+,19-,21-;/m1./s1
|
| Chemical Name |
(2S,3R,4S,5S,6R)-2-[5,7-dihydroxy-2-(4-hydroxyphenyl)chromenylium-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol;chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (~213.3 mM)
H2O: ~50 mg/mL (~106.7 mM; with ultrasonication) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.33 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.33 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1329 mL | 10.6646 mL | 21.3292 mL | |
| 5 mM | 0.4266 mL | 2.1329 mL | 4.2658 mL | |
| 10 mM | 0.2133 mL | 1.0665 mL | 2.1329 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
