| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 2mg | |||
| Other Sizes |
| Targets |
Calcitriol Impurities D is a related substance to calcitriol, which is a vitamin D receptor (VDR) agonist. As an impurity, its biological activity is not the primary focus of research; its identification and quantification are important for ensuring the purity and potency of calcitriol drug products.
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|---|---|
| ln Vitro |
In vitro, Calcitriol Impurities D is used as a reference standard in analytical methods for the quality control of calcitriol. Its presence is monitored to ensure the purity of the final product. It is not typically studied for its own biological activity.
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| ln Vivo |
In vivo, Calcitriol Impurities D is not administered as a therapeutic agent. Its relevance is in the context of the safety and efficacy of calcitriol-containing pharmaceuticals, where controlling impurity levels is critical.
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| Enzyme Assay |
In vitro analytical assays for Calcitriol Impurities D involve chromatographic techniques such as HPLC. These methods are used to separate and quantify the impurity in calcitriol drug substance and drug products to ensure they meet quality standards.
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| Cell Assay |
Cellular assays are not applicable for Calcitriol Impurities D, as it is not a drug candidate but a quality control standard. Its use is in analytical chemistry rather than cell-based pharmacology.
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| Animal Protocol |
In vivo animal studies are not applicable for Calcitriol Impurities D. Its significance is purely in the context of pharmaceutical quality control, not in pharmacological or toxicological testing as a standalone entity.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Calcitriol Impurities D are not studied, as it is not a therapeutic agent. It is a chemical impurity with a defined molecular weight of 430.66 and formula of C28H46O3.
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| Toxicity/Toxicokinetics |
The toxicological profile of Calcitriol Impurities D is not a primary focus of research. Its control is important for ensuring the safety of calcitriol products. It is not intended for human therapeutic use and is supplied for research purposes only.
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| Additional Infomation |
24-High-1,25-Dihydroxyvitamin D3 is a novel [vitamin D] analogue.
Calcitriol Impurities D is an impurity of calcitriol, the active form of vitamin D. It is used as a reference standard for quality control in pharmaceutical manufacturing. It is a by-product formed during synthesis or degradation and is available for research purposes only. |
| Molecular Formula |
C28H46O3
|
|---|---|
| Molecular Weight |
430.66304
|
| Exact Mass |
430.345
|
| CAS # |
103656-40-2
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| PubChem CID |
9547481
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| Appearance |
White to light yellow solid powder
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| Density |
1.055g/cm3
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| Boiling Point |
575.634°C at 760 mmHg
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| Flash Point |
240.469°C
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| LogP |
6.094
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| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
31
|
| Complexity |
703
|
| Defined Atom Stereocenter Count |
6
|
| SMILES |
C[C@H](CCCCC(C)(C)O)[C@H]1CC[C@@H]\2[C@@]1(CCC/C2=C\C=C/3\C[C@H](C[C@@H](C3=C)O)O)C
|
| InChi Key |
WKTIGYIYJKESCJ-JJWMBMNSSA-N
|
| InChi Code |
InChI=1S/C28H46O3/c1-19(9-6-7-15-27(3,4)31)24-13-14-25-21(10-8-16-28(24,25)5)11-12-22-17-23(29)18-26(30)20(22)2/h11-12,19,23-26,29-31H,2,6-10,13-18H2,1,3-5H3/b21-11+,22-12-/t19-,23-,24-,25+,26+,28-/m1/s1
|
| Chemical Name |
(1R,3S,5Z)-5-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-7-hydroxy-7-methyloctan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3220 mL | 11.6101 mL | 23.2202 mL | |
| 5 mM | 0.4644 mL | 2.3220 mL | 4.6440 mL | |
| 10 mM | 0.2322 mL | 1.1610 mL | 2.3220 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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