As an industrial solvent, butyl carbitol acetate does not have a specific pharmacological target. Its primary applications are as a solvent and penetrant in paints, coatings, cleaning products, and textiles. The compound may interact with biological systems through non-specific mechanisms.

Butyl carbitol acetate itself is not known to possess direct therapeutic activity. Its significance is as an industrial solvent and penetrant. The compound enables deep permeation into textiles due to its low vapor pressure and boiling point. It is used as a solvent in paints, coatings, and cleaning products.

There is no reported in vivo therapeutic activity for butyl carbitol acetate. The compound is primarily used as an industrial solvent in paints, coatings, cleaning products, and textiles. Its role in biological systems is as a solvent rather than a pharmacologically active compound.

As an industrial solvent, there are no standardized in vitro enzyme/receptor binding assays for butyl carbitol acetate. The compound's utility is in industrial applications such as paints, coatings, and cleaning products. Researchers may study the compound's physical and chemical properties, such as solubility, vapor pressure, and boiling point.

There are no reported in vitro cellular assays for butyl carbitol acetate as a therapeutic agent. The compound is used in industrial applications. Cellular studies may be conducted to assess the compound's toxicity or effects on cell viability, but these are not therapeutic assays.

There are no reported in vivo animal studies for butyl carbitol acetate as a therapeutic agent. The compound is primarily used in industrial applications. Animal studies may be conducted to assess the compound's toxicity, but these are not therapeutic studies.

Absorption, Distribution and Excretion
When diethylene glycol butyl ether (DGBE) and diethylene glycol monobutyl ether acetate (DGBEA) were applied to the skin and then washed off after 5 minutes, most (90%) of the substance was recovered. After 24 hours of administration under closed conditions, the calculated absorption rate of DGBEA (male 1.58 mg/cm²/hr, female 1.28 mg/cm²/hr; mean = 1.43) was similar to that of DGBE (male 0.73 mg/cm²/hr, female 1.46 mg/cm²/hr; mean = 1.10 mg/cm²/hr). DGBEA is rapidly absorbed from the gastrointestinal tract and is primarily (85% of the dose) excreted in the urine after metabolism. Following oral administration of 200 or 2000 mg/kg of 14C-DGBA, it is rapidly absorbed from the gastrointestinal tract and primarily excreted in the urine of rats within 24 hours. The main urinary metabolite was 2-(2-butoxyethoxy)acetic acid. No unmetabolized DGBA or DGBE was detected in rat urine at either dose level. Metabolites/Metabolites: Following transdermal administration of diethylene glycol monobutyl ether acetate, the main urinary metabolite detected was 2-(2-butoxyethoxy)acetic acid (50-60%), along with a small amount of glucuronide conjugates of DGBE (5-8%). This study investigated the in vitro hydrolysis of diethylene glycol monobutyl ether acetate (DGBA) in rat blood and its metabolism and distribution in male Sprague-Dawley rats. DGBA (5 mM) is hydrolyzed in rat blood to diethylene glycol monobutyl ether (DGBE) with a half-life of less than 3 minutes. 14C-DGBA is rapidly absorbed from the gastrointestinal tract and is primarily excreted in rat urine within 24 hours after oral administration of 200 or 2000 mg/kg. The primary urinary metabolite is 2-(2-butoxyethoxy)acetic acid. Unmetabolized DGBA or DGBE was not detected in rat urine at either dose level.

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Biological half-life
DGBA (5 mM) is hydrolyzed in rat blood to diethylene glycol monobutyl ether (DGBE), with a half-life of less than 3 minutes.

Butyl carbitol acetate has a molecular weight of 204.26 and a molecular formula of C10H20O4. It is a colorless liquid with a faint, fruity smell, a melting point of -32°C, a boiling point of 245°C, and a density of 0.977 g/mL at 20°C. The compound has low vapor pressure.

Toxicity Data
LC50 (Rat) = 72,500 mg/m³/4h

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Butyl carbitol acetate is considered safe for its intended industrial uses. The compound should be handled with appropriate safety precautions, including the use of personal protective equipment. Toxicological studies support its use in industrial applications.

Diethylene glycol monobutyl ether acetate is a colorless liquid with a slight odor. It floats on water and dissolves slowly. (US Coast Guard, 1999)
Butyl carbitol acetate (2-(2-butoxyethoxy)ethyl acetate, diethylene glycol monobutyl ether acetate) is a high-performance glycol ether ester used as a solvent in paints, coatings, cleaning products, and textiles. It is a colorless liquid with a faint, fruity smell, a boiling point of 245°C, and a density of 0.977 g/mL. The compound enables deep permeation into textiles due to its low vapor pressure.

C10H20O4

204.27

204.136

124-17-4

31288

Colorless liquid

0.977 g/mL at 20 °C(lit.)

245 °C(lit.)

-32 °C

105 °C

8.03E-06mmHg at 25°C

n20/D 1.426

1.382

0

4

10

14

136

0

CCCCOCCOCCOC(=O)C

VXQBJTKSVGFQOL-UHFFFAOYSA-N

InChI=1S/C10H20O4/c1-3-4-5-12-6-7-13-8-9-14-10(2)11/h3-9H2,1-2H3

2-(2-butoxyethoxy)ethyl acetate

NSC-5175; NSC 5175; Butyl carbitol acetate

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.

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Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)
*Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution.
Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)
Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil)
Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals).

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Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)]
*Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.
Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline)
Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline)
Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline)
Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline
Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil)
Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)

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Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium)
Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose
Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals).

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Oral Formulation 3: Dissolved in PEG400
Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose
Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose
Oral Formulation 6: Mixing with food powders

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Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.

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 (Please use freshly prepared in vivo formulations for optimal results.)