| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Thiol groups (-SH), primarily on cysteine residues in proteins and glutathione. The maleimide-like or disulfide-based reactive group on BODIPY Green 8-P2M undergoes Michael addition or disulfide exchange with thiols, activating fluorescence.
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|---|---|
| ln Vitro |
BODIPY Green 8-P2M exhibits strong fluorescence quenching in its unreacted state (d-PeT mechanism). Upon reaction with thiols (e.g., glutathione, cysteine, or protein thiols, 0.1-100 microM), fluorescence increases by 50- to 200-fold. The activated dye has excitation/emission maxima typical of BODIPY green (ex~488-505 nm, em~510-525 nm).
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| ln Vivo |
Not applicable; this is a fluorescent labeling reagent for research use. BODIPY Green 8-P2M-labeled proteins or peptides can be used for in vivo imaging of thiol-rich environments (e.g., tumors, inflamed tissues), but the free probe is not administered therapeutically.
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| Enzyme Assay |
BODIPY Green 8-P2M (1-10 microM in DMSO) is incubated with purified protein (0.1-1 mg/mL) in PBS buffer (pH 7.4) or labeling buffer (50 mM Tris-HCl, pH 7.5, 1 mM EDTA) at 25degC for 30-60 minutes. Fluorescence is measured (ex=480-500 nm, em=515-525 nm) before and after thiol reaction. Unreacted probe is removed by desalting or dialysis.
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| Cell Assay |
Cells (e.g., HeLa, Jurkat, 1×10^6 cells) are treated with BODIPY Green 8-P2M (1-10 microM) in serum-free medium for 15-60 minutes at 37degC. After washing, cells are analyzed by flow cytometry (FITC channel) or fluorescence microscopy. Alternatively, cells are treated with thiol-modulating agents (e.g., NEM, DTT, H2O2) to alter thiol availability.
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| Animal Protocol |
Not applicable as a drug. For detecting low-abundance proteins, BODIPY Green 8-P2M is used to stain SDS-PAGE gels after protein separation. Gels are incubated with 1-10 microM probe in PBS for 30-60 minutes, then destained and imaged on a fluorescence gel scanner (ex=488 nm, em=525 nm) to detect proteins down to low nanogram levels.
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| ADME/Pharmacokinetics |
Not applicable for the free probe. The BODIPY Green 8-P2M dye has excellent photostability and low non-specific binding. Its high signal-to-noise ratio (50-200:1 upon thiol reaction) makes it suitable for detecting low-abundance thiol-containing proteins even in complex mixtures.
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| Toxicity/Toxicokinetics |
No toxicity; BODIPY Green 8-P2M is used at low micromolar concentrations for labeling and shows minimal cellular toxicity. As with all fluorescent probes, it should be handled with care and protected from light to prevent photobleaching.
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| References | |
| Additional Infomation |
BODIPY Green 8-P2M is for research use only, not FDA-approved. Its extremely high signal-to-noise ratio makes it ideal for detecting low-concentration proteins in SDS-PAGE gels and for visualizing thiols in live cells. It is a novel thiol-reactive probe that overcomes the limitations of traditional thiol dyes (e.g., monobromobimane, ThioGlo) by providing turn-on fluorescence only upon thiol reaction, minimizing background signal.
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| Molecular Formula |
C23H20BN3O2F2
|
|---|---|
| Molecular Weight |
419.232
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| Exact Mass |
419.162
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| CAS # |
929679-22-1
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| PubChem CID |
102513182
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| Appearance |
Brown to red solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
31
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| Complexity |
938
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1C=C(C)[N-]2[B+3]([F-])([F-])N3=C(C)C=C(C)C3=C(C3C=CC=CC=3N3C(C=CC3=O)=O)C=12
|
| InChi Key |
SJOIHAOAQXENHO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C23H20BF2N3O2/c1-13-11-15(3)28-22(13)21(23-14(2)12-16(4)29(23)24(28,25)26)17-7-5-6-8-18(17)27-19(30)9-10-20(27)31/h5-12H,1-4H3
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| Chemical Name |
1-[2-(2,2-difluoro-4,6,10,12-tetramethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-8-yl)phenyl]pyrrole-2,5-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~119.27 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3853 mL | 11.9266 mL | 23.8533 mL | |
| 5 mM | 0.4771 mL | 2.3853 mL | 4.7707 mL | |
| 10 mM | 0.2385 mL | 1.1927 mL | 2.3853 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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