| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
BODIPY-FL does not have a specific biological target as a drug; it is a fluorescent dye. It can be used to label probes or primers for the quantitative detection of specific DNA/RNA based on fluorescence quenching. BODIPY-FL-labeled monoterpenes can be used to examine the characteristics of Gram-positive and Gram-negative bacteria and pathogenic fungi.
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| ln Vitro |
BODIPY-FL is not evaluated for biological activity as a drug; it is a fluorescent dye. Its utility is assessed by its fluorescence properties, including a quantum yield of up to 0.97, excitation at 503-505 nm, and emission at 509-515 nm. It is used in fluorescence-based assays for labeling and detection.
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| ln Vivo |
BODIPY-FL is not evaluated for in vivo biological activity as a drug; it is a fluorescent probe. It may be used in vivo for imaging applications, such as monitoring the uptake and trafficking of BODIPY-labeled proteins and other compounds within cells by fluorescence microscopy.
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| Enzyme Assay |
There are no specific in vitro enzyme or receptor binding assays for BODIPY-FL as a drug target. As a fluorescent dye, its properties are characterized by absorbance and fluorescence spectra.
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| Cell Assay |
There are no specific in vitro cellular assays for BODIPY-FL as a pharmacologically active compound. As a fluorescent dye, it is used to label probes or primers for the quantitative detection of specific DNA/RNA. Cells are incubated with the labeled molecule, and fluorescence is visualized by microscopy or flow cytometry.
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| Animal Protocol |
There are no specific in vivo animal studies for BODIPY-FL as a pharmacologically active compound. As a fluorescent probe, it may be used for in vivo imaging studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of BODIPY-FL are not applicable, as it is a fluorescent dye rather than a drug. The compound is not intended for administration as a therapeutic agent.
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| Toxicity/Toxicokinetics |
The toxicity profile of BODIPY-FL is not extensively reported. As a fluorescent dye, it is not intended for human therapeutic use. The compound should be handled with care in a laboratory setting.
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| References | |
| Additional Infomation |
BODIPY FL is a BODIPY dye and a monocarboxylic acid. It is used as a fluorescent dye. It is functionally related to 4,4-difluoro-4-boron-3a,4a-diaza-s-indendrobenzene.
BODIPY-FL (CAS 165599-63-3) is a bright green-fluorescent dye belonging to the BODIPY family. It has a molecular formula of C14H15BF2N2O2 and a molecular weight of 292.09. The compound is used to label probes or primers for DNA/RNA detection and for imaging applications. It is available for research purposes only. |
| Molecular Formula |
C14H15BF2N2O2
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|---|---|
| Molecular Weight |
292.0889
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| Exact Mass |
292.119
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| CAS # |
165599-63-3
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| PubChem CID |
9817511
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| Appearance |
Brown to red solid powder
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| Melting Point |
196 °C
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| LogP |
1.836
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
21
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| Complexity |
598
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
BJDJEJIINKBPHY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H15BF2N2O2/c1-9-7-10(2)18-13(9)8-12-4-3-11(5-6-14(20)21)19(12)15(18,16)17/h3-4,7-8H,5-6H2,1-2H3,(H,20,21)
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| Chemical Name |
3-(2,2-difluoro-10,12-dimethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl)propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~342.36 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4236 mL | 17.1180 mL | 34.2360 mL | |
| 5 mM | 0.6847 mL | 3.4236 mL | 6.8472 mL | |
| 10 mM | 0.3424 mL | 1.7118 mL | 3.4236 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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