| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Carbonyl groups (aldehydes, ketones) on polysaccharides, glycoproteins, and other biomolecules. This includes oxidized sugars, reducing sugars, and carbonyls generated by oxidative stress or chemical modification.
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|---|---|
| ln Vitro |
BODIPY FL hydrazide is a bright and photostable dye with quantum yield approaching unity and excellent photostability. It reacts with aldehydes/ketones on polysaccharides and glycoproteins to form a reversible Schiff base product, which can be stabilized with reducing agents (sodium borohydride or sodium cyanoborohydride) to form a stable linker.
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| ln Vivo |
Not applicable; this is a fluorescent labeling reagent for research use. Conjugates labeled with BODIPY FL hydrazide can be used for in vivo imaging of glycoproteins or oxidative stress markers, but the free hydrazide is not administered therapeutically.
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| Enzyme Assay |
BODIPY FL hydrazide (1-10 mM in DMSO or DMF) is diluted to 10-100 microM in labeling buffer (0.1 M sodium acetate, pH 5.5-6.0, or 0.1 M phosphate buffer, pH 7.0-7.4). The aldehyde-containing biomolecule (0.1-10 mg/mL) is added and incubated at 25degC for 2-24 hours. Sodium cyanoborohydride (10-50 mM) may be added to reduce the Schiff base to a stable amine.
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| Cell Assay |
Cells are treated with H2O2 or other oxidants to induce oxidative stress and generate carbonyl groups. BODIPY FL hydrazide (1-10 microM) is added to live or fixed cells for 30-60 minutes at 37degC. After washing, cells are analyzed by flow cytometry (FL1 channel) or fluorescence microscopy to detect protein carbonylation or glycoprotein labeling.
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| Animal Protocol |
Not applicable as a drug. For imaging applications, BODIPY FL hydrazide-labeled glycoproteins (e.g., glycosylated antibodies) are injected intravenously into mice (1-10 mg/kg). Fluorescence imaging (ex=495 nm, em=516 nm) is performed at 1-24 hours post-injection to study glycoprotein distribution and tumor targeting.
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| ADME/Pharmacokinetics |
Not applicable for the free hydrazide. BODIPY FL-labeled conjugates exhibit excellent photostability and serum stability. The dye is non-toxic and does not interfere with protein function at low labeling ratios (dye:protein <5:1). Hydrazide-labeled compounds have half-lives determined by the carrier molecule.
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| Toxicity/Toxicokinetics |
No toxicity; BODIPY FL hydrazide is used at low concentrations (1-10 microM) for labeling and shows minimal cytotoxicity. Avoid prolonged light exposure during experiments to prevent photobleaching. Handle as a standard fluorescent dye with protective equipment.
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| References |
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| Additional Infomation |
BODIPY FL hydrazide is for research use only, not FDA-approved. It is widely used for detecting glycoproteins (Western blotting, ELISA), labeling oxidized proteins in oxidative stress studies, and preparing fluorescent probes for cell surface aldehyde tags. It is also known as BDP FL hydrazide.
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| Molecular Formula |
C14H17BN4OF2
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|---|---|
| Molecular Weight |
306.119
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| Exact Mass |
306.146
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| CAS # |
178388-71-1
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| Related CAS # |
2183473-45-0 (hydrochloride); 178388-71-1; 2256724-38-4;
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| PubChem CID |
101422477
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| Appearance |
Brown to red solid powder
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
602
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(C1=CC=C2C=C3C(C)=CC(C)=N3[B+3]([F-])([F-])[N-]12)CC(=O)NN
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| InChi Key |
WFYHYVNCFIXKNN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H17BF2N4O/c1-9-7-10(2)20-13(9)8-12-4-3-11(5-6-14(22)19-18)21(12)15(20,16)17/h3-4,7-8H,5-6,18H2,1-2H3,(H,19,22)
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| Chemical Name |
3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)propanehydrazide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~408.34 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2667 mL | 16.3335 mL | 32.6669 mL | |
| 5 mM | 0.6533 mL | 3.2667 mL | 6.5334 mL | |
| 10 mM | 0.3267 mL | 1.6333 mL | 3.2667 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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