| Size | Price | Stock | Qty |
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| 1mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| ln Vitro |
How to apply 1. 1.1 Preparation of stock solution: Dissolve 1 milligram of BODIPY 493/503 in 382 μL DMSO to create a 10 mM stock solution. This completes the preparation of the working solution. Note: It is advised to keep the stock solution between -20°C and -80°C in storage. To obtain a 1-10 μM working solution, do not use 1.2 BODIPY 493/503 working solution without bone marrow or PBS extraction solution. Note: Please modify the working solution for BODIPY 493/503 based on the current circumstances. 2. Suspension cells (6-well plate) 2.1 Cell staining a. Discard the supernatant after centrifuging at 1000g for three to five minutes at 4°C. Use PBS to wash twice, for five minutes each. There is b cell density. For five to thirty minutes, add one milliliter of the working solution. 1×106/mL. Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. d. Use PBS to wash twice, for five minutes each time. e. Re-suspend the cells in PBS or serum-free cells. 2.2 Adherent cells as observed using a flow cytometer or fluorescence microscopy a. On sterile coverslips, cultivate adherent cells. b. Empty the coverslip of any extra cells by removing it from the culture medium. C. To fully cover it, add 100 μL of working solution and give it a little shake. Prelude: 5–30 minutes for cells. d. Use water to wash twice, for five minutes each time. observation using flow cytometry or fluorescence microscopy. For a year, store at -20°C; shield from light. Observations 1. Kindly modify the BODIPY 493/503 working fluid concentration based on the current circumstances. 2. Prior to conducting the next studies, Saudi cells were first cultured with 30 μM oleic acid for eight hours. 3. The creator created and utilized this product; it is not intended for use as a home or medical tool. 4. When working, please use a lab coat and disposable gloves for your own health and safety.
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| References |
| Molecular Formula |
C14H17BF2N2
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|---|---|
| Molecular Weight |
262.1060
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| Exact Mass |
262.145
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| CAS # |
121207-31-6
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| PubChem CID |
14766991
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| Appearance |
Brown to reddish brown solid powder
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| Melting Point |
257 °C
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| LogP |
2.826
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
19
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| Complexity |
505
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
DRJHPEGNOPSARR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H17BF2N2/c1-8-6-10(3)18-13(8)12(5)14-9(2)7-11(4)19(14)15(18,16)17/h6-7H,1-5H3
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| Chemical Name |
2,2-difluoro-4,6,8,10,12-pentamethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~19.08 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8152 mL | 19.0760 mL | 38.1519 mL | |
| 5 mM | 0.7630 mL | 3.8152 mL | 7.6304 mL | |
| 10 mM | 0.3815 mL | 1.9076 mL | 3.8152 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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