BODIPY-493/503 targets neutral lipids, particularly lipid droplets, in cells. It is a lipophilic dye that stains cellular neutral lipid content. Its fluorescence properties allow for the visualization and quantification of lipid droplets. The dye is relatively insensitive to environmental polarity and pH, making it stable under different physiological conditions.

How to apply 1. 1.1 Preparation of stock solution: Dissolve 1 milligram of BODIPY 493/503 in 382 μL DMSO to create a 10 mM stock solution. This completes the preparation of the working solution. Note: It is advised to keep the stock solution between -20°C and -80°C in storage. To obtain a 1-10 μM working solution, do not use 1.2 BODIPY 493/503 working solution without bone marrow or PBS extraction solution. Note: Please modify the working solution for BODIPY 493/503 based on the current circumstances. 2. Suspension cells (6-well plate) 2.1 Cell staining a. Discard the supernatant after centrifuging at 1000g for three to five minutes at 4°C. Use PBS to wash twice, for five minutes each. There is b cell density. For five to thirty minutes, add one milliliter of the working solution. 1×106/mL. Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. d. Use PBS to wash twice, for five minutes each time. e. Re-suspend the cells in PBS or serum-free cells. 2.2 Adherent cells as observed using a flow cytometer or fluorescence microscopy a. On sterile coverslips, cultivate adherent cells. b. Empty the coverslip of any extra cells by removing it from the culture medium. C. To fully cover it, add 100 μL of working solution and give it a little shake. Prelude: 5–30 minutes for cells. d. Use water to wash twice, for five minutes each time. observation using flow cytometry or fluorescence microscopy. For a year, store at -20°C; shield from light. Observations 1. Kindly modify the BODIPY 493/503 working fluid concentration based on the current circumstances. 2. Prior to conducting the next studies, Saudi cells were first cultured with 30 μM oleic acid for eight hours. 3. The creator created and utilized this product; it is not intended for use as a home or medical tool. 4. When working, please use a lab coat and disposable gloves for your own health and safety.
In vitro, BODIPY-493/503 is used to label lipid droplets in live and fixed cells. It is compatible with fluorescence microscopy, confocal and two-photon microscopy, and flow cytometry. It can be used for live-cell and fixed-cell applications. The dye can be used to facilitate quantification of neutral lipid content by flow cytometry and observation of lipid droplets by microscopy.

BODIPY-493/503 is not used as a therapeutic agent. Its primary application is as a research tool for lipid staining and imaging in vitro.

In vitro non-cell-based assays for BODIPY-493/503 are not typical, as it is a cell-permeant dye used for staining. Its fluorescence properties can be characterized in solution to confirm its excitation and emission spectra.

In vitro cell-based assays using BODIPY-493/503 involve staining cells with the dye. Cells are incubated with the dye, and after washing, the fluorescence is visualized using fluorescence microscopy or quantified by flow cytometry. The dye is used to study lipid metabolism and the formation of lipid droplets. It can be used for both live and fixed cells.

BODIPY-493/503 is not typically used for in vivo animal experiments. Its application is primarily in in vitro cell staining and imaging.

BODIPY-493/503 has the molecular formula C14H17BF2N2 and a molecular weight of 262.11 g/mol. It is a brown to reddish brown solid powder with a purity of ≥99%. It is soluble in DMSO. It is also known as Pyrromethene 546 and BDP 493/503 lipid stain.

BODIPY-493/503 is generally considered to have low toxicity as a fluorescent dye for cell staining. Standard laboratory safety precautions should be followed when handling it.

[1]. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.

BODIPY-493/503 is a lipophilic fluorescent probe for staining neutral lipids and lipid droplets. It is compatible with various imaging techniques. It is intended for research use.

C14H17BF2N2

262.1060

262.145

121207-31-6

14766991

Brown to reddish brown solid powder

257 °C

2.826

0

3

0

19

505

0

DRJHPEGNOPSARR-UHFFFAOYSA-N

InChI=1S/C14H17BF2N2/c1-8-6-10(3)18-13(8)12(5)14-9(2)7-11(4)19(14)15(18,16)17/h6-7H,1-5H3

2,2-difluoro-4,6,8,10,12-pentamethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO : ~5 mg/mL (~19.08 mM)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.

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Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)
*Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution.
Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)
Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil)
Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals).

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Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)]
*Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.
Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline)
Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline)
Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline)
Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline
Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil)
Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)

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Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium)
Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose
Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals).

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Oral Formulation 3: Dissolved in PEG400
Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose
Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose
Oral Formulation 6: Mixing with food powders

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Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.

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 (Please use freshly prepared in vivo formulations for optimal results.)