| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg | |||
| Other Sizes |
| Targets |
Bispyribac-sodium targets acetolactate synthase (ALS), also known as acetohydroxyacid synthase (AHAS), a key enzyme in the biosynthesis of the branched-chain amino acids valine, leucine, and isoleucine. This enzyme is present in plants but not in animals, which confers the herbicide's selective toxicity. By inhibiting ALS, Bispyribac-sodium prevents the production of these essential amino acids, leading to a halt in plant growth and development. The plant stops growing shortly after application and eventually dies. Bispyribac-sodium's mode of action is through the inhibition of this critical metabolic pathway.
|
|---|---|
| ln Vitro |
In vitro, Bispyribac-sodium is a potent inhibitor of acetolactate synthase (ALS) activity. Its activity is typically measured using enzyme assays with ALS extracted from plant tissues. The enzyme is incubated with its substrate, pyruvate, in the presence of varying concentrations of the herbicide. The production of acetolactate, the product of the ALS reaction, is measured, and the IC50 is determined. These assays confirm the compound's mechanism of action as an ALS inhibitor.
|
| ln Vivo |
In vivo, Bispyribac-sodium is used as a post-emergent herbicide in rice paddies. It is absorbed by the foliage and roots of target weeds, and it is translocated throughout the plant, accumulating in meristematic tissues. The herbicide inhibits the growth of susceptible weeds, which stop growing shortly after application. Bispyribac-sodium is effective against a wide range of grass and broadleaf weeds, including Echinochloa crus-galli (barnyardgrass) and various sedges. Its efficacy is influenced by environmental factors such as temperature and soil conditions.
|
| Enzyme Assay |
In vitro enzyme assays for Bispyribac-sodium measure its inhibition of acetolactate synthase (ALS). The enzyme is typically extracted from plant tissues, such as maize or Arabidopsis thaliana. The assay mixture contains the enzyme, its substrate pyruvate, the cofactor thiamine pyrophosphate (TPP), and varying concentrations of the herbicide. After incubation, the reaction is stopped, and the amount of acetolactate produced is measured colorimetrically after acidification and decarboxylation to acetoin. The IC50 is determined from the dose-response curve. This assay confirms the compound's herbicidal mechanism of action.
|
| Cell Assay |
In vitro cell-based assays for Bispyribac-sodium are conducted using plant cell cultures or seedlings. Plant cells or seedlings are treated with the herbicide at various concentrations. The inhibition of growth or the accumulation of branched-chain amino acids can be measured. The compound's effects on plant cell division and metabolism can be assessed. These assays confirm the herbicidal activity of Bispyribac-sodium at the cellular level.
|
| Animal Protocol |
In vivo animal experiments for Bispyribac-sodium are conducted to assess its toxicological profile and environmental fate. In a typical study, the herbicide is administered to rodents orally, and its acute and chronic toxicity is evaluated. Parameters such as body weight, food consumption, and organ histopathology are assessed. The herbicide's metabolism and excretion are also studied. These studies provide data on the compound's safety for non-target organisms and its environmental persistence.
|
| ADME/Pharmacokinetics |
Bispyribac-sodium has a molecular weight of 452.35 g/mol and a molecular formula of C19H18N4NaO8. It is a solid compound. It is soluble in water and organic solvents. For storage, it is recommended to keep the compound in a cool, dry place. Detailed pharmacokinetic properties are not relevant as it is a herbicide, not a therapeutic agent. Its environmental fate, including degradation and mobility in soil and water, is an important aspect of its profile.
|
| Toxicity/Toxicokinetics |
Bispyribac-sodium is a herbicide and its toxicity has been studied in animal models. It is of low acute toxicity to mammals and birds. The compound is practically non-toxic to fish and aquatic invertebrates. However, it may be moderately toxic to some aquatic plants. Appropriate safety measures, including the use of personal protective equipment, should be taken when handling Bispyribac-sodium to avoid exposure. It is a restricted-use pesticide in some countries.
|
| References | |
| Additional Infomation |
Bispyribac is the organosodium salt of Bispyribac. It is a broad-spectrum post-emergence herbicide used to control grasses, sedges, and broadleaf weeds in rice paddies. It is a herbicide, an agricultural chemical, and also an environmental pollutant. It contains a Bispyribac (1-) group.
See also: Bispyribac (note moved to). Bispyribac-sodium is a broad-spectrum, post-emergent herbicide used for the control of grasses, sedges, and broadleaf weeds in rice crops. It is a systemic herbicide that is absorbed through the leaves, stems, and roots and is translocated throughout the plant. Its mechanism of action involves the inhibition of acetolactate synthase (ALS), a key enzyme in the biosynthesis of branched-chain amino acids. Bispyribac-sodium is a commercial herbicide and is not a therapeutic drug. |
| Molecular Formula |
C19H17N4NAO8
|
|---|---|
| Molecular Weight |
452.3548
|
| Exact Mass |
452.094
|
| CAS # |
125401-92-5
|
| Related CAS # |
125401-75-4 (free acid);125401-92-5 (sodium);
|
| PubChem CID |
23682789
|
| Appearance |
White to off-white solid powder
|
| Density |
1.379 g/cm3
|
| Boiling Point |
686.4ºC at 760 mmHg
|
| Melting Point |
223-224°C
|
| Flash Point |
368.9ºC
|
| LogP |
1.249
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
9
|
| Heavy Atom Count |
32
|
| Complexity |
515
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
FUHMZYWBSHTEDZ-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C19H18N4O8.Na/c1-26-12-8-13(27-2)21-18(20-12)30-10-6-5-7-11(16(10)17(24)25)31-19-22-14(28-3)9-15(23-19)29-4;/h5-9H,1-4H3,(H,24,25);/q;+1/p-1
|
| Chemical Name |
sodium;2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoate
|
| Synonyms |
Bispyribac-sodium V 10029 V-10029 V10029
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~11.05 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2107 mL | 11.0534 mL | 22.1068 mL | |
| 5 mM | 0.4421 mL | 2.2107 mL | 4.4214 mL | |
| 10 mM | 0.2211 mL | 1.1053 mL | 2.2107 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
