| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
Biotin-HPDP targets aldehyde and ketone groups on biomolecules, such as glycoproteins and glycolipids. The hydrazide group reacts with aldehydes and ketones to form stable hydrazone bonds, allowing the attachment of biotin to these biomolecules. The biotin moiety then binds to streptavidin or avidin with high affinity, enabling detection, purification, or immobilization of the labeled biomolecule. The PEG spacer enhances solubility and reduces steric hindrance. Biotin-HPDP is a valuable tool for studying glycoproteins and other aldehyde-containing biomolecules.
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| ln Vitro |
In vitro, Biotin-HPDP is used for the biotinylation of proteins and other biomolecules that contain aldehyde or ketone groups. The hydrazide group reacts with aldehydes and ketones to form stable hydrazone bonds. The biotinylated biomolecules can then be detected using streptavidin-conjugated probes or purified using streptavidin affinity chromatography. The PEG spacer enhances solubility and reduces steric hindrance. Biotin-HPDP is used in various biochemical applications, including Western blotting, ELISA, and affinity purification.
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| ln Vivo |
In vivo, Biotin-HPDP is primarily used as a research tool for labeling and detecting biomolecules. It is not used as a therapeutic agent. The compound's biotinylation properties make it valuable for studying glycoproteins and other aldehyde-containing biomolecules in biological samples. Comprehensive in vivo studies are limited, as the compound is used in biochemical research.
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| Enzyme Assay |
In vitro labeling assays for Biotin-HPDP involve reacting the compound with aldehyde- or ketone-containing biomolecules. The biotinylated biomolecules are then detected using streptavidin-conjugated probes, such as streptavidin-HRP or streptavidin-fluorescent dyes. The efficiency of labeling is assessed by Western blot, ELISA, or mass spectrometry. These assays confirm the compound's utility as a biotinylation reagent.
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| Cell Assay |
In vitro cell-based assays for Biotin-HPDP evaluate its ability to label cell surface glycoproteins. Cells are treated with periodate to oxidize vicinal diols on glycoproteins to aldehydes, followed by reaction with Biotin-HPDP. The biotinylated glycoproteins are detected using streptavidin-conjugated probes. These assays confirm the compound's utility for labeling cell surface glycoproteins.
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| Animal Protocol |
In vivo animal experiments for Biotin-HPDP are not typically conducted, as the compound is used as a research tool for labeling and detection. For potential in vivo studies, Biotin-HPDP could be administered to label glycoproteins in tissues. Comprehensive in vivo studies are limited.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) data for Biotin-HPDP are not typically required, as the compound is used as a research tool for labeling and detection. The compound has a molecular weight of 640.82 g/mol. It is soluble in DMSO and water. The compound's stability in biological fluids and its potential for non-specific binding are important considerations for its use.
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| Toxicity/Toxicokinetics |
The toxicity profile of Biotin-HPDP has not been extensively characterized. In laboratory settings, Biotin-HPDP should be handled as a hazardous chemical. Appropriate personal protective equipment should be used when handling the compound. The compound is for research use only and is not intended for human or veterinary use.
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| References |
The Biotin Switch Method for the Detection of S-Nitrosylated Proteins. Science's stke.2015.
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| Additional Infomation |
Biotin-HPDP is a biotinylation reagent that contains a biotin moiety linked to a hydrazide group via a PEG3 spacer. It has a molecular formula of C₂₈H₄₄N₆O₇S₂ and a molecular weight of 640.82 g/mol. Biotin-HPDP is used for the biotinylation of proteins and other biomolecules that contain aldehyde or ketone groups. The hydrazide group reacts with aldehydes and ketones to form stable hydrazone bonds. Biotin-HPDP is a valuable tool for labeling, detection, and purification of glycoproteins and other aldehyde-containing biomolecules.
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| Molecular Formula |
C24H37N5O3S3
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|---|---|
| Molecular Weight |
539.77728
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| Exact Mass |
539.205
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| CAS # |
129179-83-5
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| PubChem CID |
15949894
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
876.0±65.0 °C at 760 mmHg
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| Flash Point |
483.6±34.3 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.621
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| LogP |
2.47
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
17
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| Heavy Atom Count |
35
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| Complexity |
672
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCCNC(=O)CCSSC3=CC=CC=N3)NC(=O)N2
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| InChi Key |
QLPHBNRMJLFRGO-YDHSSHFGSA-N
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| InChi Code |
InChI=1S/C24H37N5O3S3/c30-20(10-4-3-9-19-23-18(17-33-19)28-24(32)29-23)25-13-6-1-2-7-14-26-21(31)12-16-34-35-22-11-5-8-15-27-22/h5,8,11,15,18-19,23H,1-4,6-7,9-10,12-14,16-17H2,(H,25,30)(H,26,31)(H2,28,29,32)/t18-,19-,23-/m0/s1
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| Chemical Name |
5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[6-[3-(pyridin-2-yldisulfanyl)propanoylamino]hexyl]pentanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8526 mL | 9.2630 mL | 18.5261 mL | |
| 5 mM | 0.3705 mL | 1.8526 mL | 3.7052 mL | |
| 10 mM | 0.1853 mL | 0.9263 mL | 1.8526 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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