| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
No classical pharmacological receptor or enzyme target. The compounds are tracing reagents: biocytin is a substrate for endogenous biotinylation and streptavidin detection, and Lucifer Yellow is a fluorescent tracer for cell labeling and gap-junction permeability.
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|---|---|
| ln Vitro |
The compounds themselves are not modulators of enzyme or receptor activity. Biocytin is a neuronal tracer that can be visualized with streptavidin-HRP or fluorescent streptavidin, and Lucifer Yellow is a fluorescent dye that labels cells and reports on gap-junction coupling.
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| ln Vivo |
In vivo, biocytin and Lucifer Yellow are used for neuronal tracing after injection into brain regions, enabling visualization of axonal projections and gap-junction coupling. They are not administered systemically for therapeutic purposes.
|
| Enzyme Assay |
For cell-free detection, biocytin can be detected by streptavidin-HRP or fluorescent streptavidin in ELISA or blotting formats. Lucifer Yellow fluorescence can be measured spectrofluorometrically (ex ~428 nm, em ~540 nm).
|
| Cell Assay |
For neuronal tracing, inject biocytin (1–5% in physiological buffer) or Lucifer Yellow (1–5% in water) into the target brain region using iontophoresis or pressure injection. After a survival period (hours to days), perfuse the animal, section the brain, and visualize biocytin with streptavidin-HRP (DAB) or fluorescent streptavidin, and Lucifer Yellow by direct fluorescence microscopy.
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| Animal Protocol |
For in vivo tracing, inject biocytin or Lucifer Yellow into the target tissue or brain region as described, then collect tissues at defined time points. Process for immunohistochemistry or fluorescence microscopy. Include injection-site controls and, for gap-junction studies, a gap-junction inhibitor as a negative control.
|
| References |
|
| Molecular Formula |
C32H44KN7O10S3
|
|---|---|
| Molecular Weight |
822.03
|
| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2165 mL | 6.0825 mL | 12.1650 mL | |
| 5 mM | 0.2433 mL | 1.2165 mL | 2.4330 mL | |
| 10 mM | 0.1217 mL | 0.6083 mL | 1.2165 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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