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| 5g | ||
| 10g | ||
| Other Sizes |
| Targets |
The molecular targets of BFMO in biological systems have not been formally characterized. As a biochemical reagent, its primary utility is in chemical synthesis and environmental applications rather than as a pharmacological agent targeting specific biological molecules. However, as an oxalamide derivative containing furan rings, BFMO may interact with various biological targets through hydrogen bonding, π-π stacking, and metal coordination. The oxalamide functionality can serve as a hydrogen bond donor and acceptor, potentially interacting with enzymes and receptors. The furan rings may undergo metabolic activation by cytochrome P450 enzymes, forming reactive epoxide intermediates that can alkylate proteins and DNA. In the context of its use as a ligand in copper-catalyzed reactions, BFMO coordinates to copper ions through the oxygen and nitrogen atoms of the oxalamide moiety, facilitating catalytic transformations. Its environmental application for arsenic removal involves adsorption and coprecipitation mechanisms rather than specific molecular targeting.
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| ln Vitro |
In vitro biological activity data for BFMO are primarily related to its role as a biochemical reagent rather than as a biologically active compound with specific pharmacological effects. The compound's ability to coordinate metal ions may influence metalloenzyme activities in vitro, potentially inhibiting or activating enzymes that require metal cofactors. Its furan rings may undergo oxidation reactions in cell culture media, generating reactive oxygen species that could affect cell viability and function. The compound may also interact with cellular membranes due to its moderate lipophilicity, potentially affecting membrane fluidity and permeability. However, specific in vitro activity data such as IC50 values against particular biological targets are not available in the literature. As a research reagent, BFMO is used primarily for chemical synthesis and environmental studies rather than for biological activity screening.
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| ln Vivo |
In vivo data for BFMO are primarily related to its environmental applications rather than pharmacological effects. BFMO (biogenic Fe-Mn oxides) has been studied for its ability to remove arsenic and other heavy metals from water. In environmental settings, BFMO can simultaneously eliminate or reduce Fe(II), Mn(II), and As(III&V) species through adsorption, oxidation, and coprecipitation mechanisms. This makes it useful for water treatment applications, particularly in groundwater remediation where high concentrations of iron, manganese, and arsenic are present. In biological systems, the compound is not intended for therapeutic use and would not be administered to animals for pharmacological evaluation. Its stability and reactivity in aqueous environments make it suitable for environmental applications, but its biological safety and efficacy in living organisms have not been systematically studied.
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| Enzyme Assay |
For in vitro experiments with BFMO as a chemical reagent, the following protocol is typically used: BFMO is dissolved in an appropriate organic solvent such as DMSO or THF at a concentration of 0.1-1.0 M. For copper-catalyzed coupling reactions, BFMO (10-20 mol%) is added to a reaction mixture containing the aryl halide (1.0 equiv), the nitrogen heterocycle (1.2 equiv), copper(I) iodide (5-10 mol%), and a base such as Cs₂CO₃ or K₂CO₃ in a suitable solvent (e.g., DMF or DMSO). The reaction is heated to 80-120°C for 12-24 hours under an inert atmosphere. The progress of the reaction is monitored by TLC or HPLC. After completion, the reaction mixture is diluted with water and extracted with ethyl acetate. The combined organic layers are dried and concentrated, and the product is purified by column chromatography. The yield and purity of the product are determined by NMR and HPLC analysis.
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| Cell Assay |
For cell-based experiments with BFMO, the following general protocol would be used if the compound were to be tested for biological activity: cells (e.g., HEK293 or HeLa) are cultured in DMEM with 10% FBS and antibiotics at 37°C in 5% CO₂. Cells are seeded in 96-well plates at 5,000-10,000 cells per well and allowed to adhere overnight. BFMO is dissolved in DMSO and diluted in culture medium to final concentrations ranging from 1 to 100 μM (final DMSO ≤ 0.1%). Cells are treated for 24-72 hours, and cell viability is assessed using the MTT or CCK-8 assay. For studying metal chelation effects, cells can be treated with BFMO in the presence of various metal ions, and cellular metal content can be measured by ICP-MS. For oxidative stress assessment, cells are loaded with DCFH-DA and fluorescence is measured after treatment. However, as BFMO is primarily used as a reagent for chemical synthesis, such cell-based studies are not standard applications of this compound.
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| Animal Protocol |
For environmental applications of BFMO, the following general protocol is used: BFMO (biogenic Fe-Mn oxides) is synthesized by culturing Mn(II)-oxidizing bacteria (e.g., Pseudomonas putida strain MnB1) or by chemical coprecipitation of Fe and Mn oxides. The resulting BFMO material is characterized by XRD, SEM, and BET surface area analysis. For arsenic removal studies, BFMO is added to water samples containing As(III) and As(V) at concentrations of 0.1-10 mg/L, along with Fe(II) and Mn(II) at various concentrations. The mixture is stirred at room temperature for 1-24 hours. Samples are collected at various time points, filtered through 0.45 μm membranes, and analyzed for residual arsenic, iron, and manganese concentrations by ICP-OES or ICP-MS. The removal efficiency is calculated as (C₀ - Cₜ)/C₀ × 100%. The adsorption capacity is determined by fitting the data to Langmuir and Freundlich isotherm models. The effect of pH, temperature, and competing ions on removal efficiency can also be evaluated.
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| Additional Infomation |
BFMO is a biochemical reagent used as a biological material or organic compound for life science research. It is used as a ligand in copper-catalyzed coupling reactions and for arsenic removal from water. BFMO has a molecular formula of C12H12N2O4, a molecular weight of 248.24 g/mol, and a purity of >98.0%. It is a solid at room temperature and should be stored as a powder at -20°C for 3 years or at 4°C for 2 years. BFMO exists as a biogenic Fe-Mn oxide mixture that can simultaneously eliminate or reduce Fe(II), Mn(II), and As(III&V) species. Future research could explore its potential in other catalytic applications, its utility in environmental remediation of other heavy metals, and its possible biological activities through systematic screening against relevant targets.
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| Molecular Formula |
C12H12N2O4
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|---|---|
| Molecular Weight |
248.23468
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| Exact Mass |
248.08
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| CAS # |
69010-90-8
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| PubChem CID |
330826
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| Appearance |
White to off-white solid powder
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| Density |
1.283g/cm3
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| Index of Refraction |
1.547
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| LogP |
1.587
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
18
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| Complexity |
279
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=COC(=C1)CNC(=O)C(=O)NCC2=CC=CO2
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| InChi Key |
XRURWFXKCKASSN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H12N2O4/c15-11(13-7-9-3-1-5-17-9)12(16)14-8-10-4-2-6-18-10/h1-6H,7-8H2,(H,13,15)(H,14,16)
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| Chemical Name |
N,N'-bis(furan-2-ylmethyl)oxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0285 mL | 20.1426 mL | 40.2852 mL | |
| 5 mM | 0.8057 mL | 4.0285 mL | 8.0570 mL | |
| 10 mM | 0.4029 mL | 2.0143 mL | 4.0285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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