| Size | Price | Stock | Qty |
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| ln Vitro |
In an in vitro insulin resistance model, β-Aminopropionitrile (BAPN) increases glucose uptake and normalizes the expression of GLUT4 and adiponectin[1]. β-Aminopropionitrile (500 μM; 72 h) suppresses cervical cancer cells' ability to invade and migrate in vitro, as well as the morphological and marker protein alterations brought on by hypoxia-induced EMT[2].
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| ln Vivo |
In rats with diet-induced obesity, β-Aminopropionitrile (BAPN) (100 mg/kg/day; po; 6 weeks) improves the metabolic profile and decreases body weight gain[1]. C57BL/6 mice are given β-Aminopropionitrile monofumarate (1 g/kg/day; po; 4 weeks) to induce thoracic aortic dissection[3].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: 3T3-L1 adipocytes Tested Concentrations: 200 μM with 1.15 nM and 2.87 nM TNFα Incubation Duration: 72 h Experimental Results: TNFα decreased expression of GLUT4 and adiponectin, and increased SOCS3 protein levels in these cells. And these effects were prevented. Cell Invasion Assay[2] Cell Types: HeLa and SiHa cells Tested Concentrations: 500 μM Incubation Duration: 72 h Experimental Results: Dramatically decreased hypoxia- elicited cell invasion in both cell models. Cell Migration Assay [2] Cell Types: HeLa and SiHa cells Tested Concentrations: 500 μM Incubation Duration: 72 h Experimental Results: diminished hypoxia-induced migration from 180 and 240% to 60 and 70% in HeLa and SiHa cells, respectively. Western Blot Analysis[2] Cell Types: HeLa and SiHa cells Tested Concentrations: 500 μM Incubation Duration: 72 h Experimental Results: Effectively prevented hypoxia-induced downregulation of E-cadherin and strongly inhibited hypoxia-induced upregulation of α-SMA and vimentin. |
| Animal Protocol |
Animal/Disease Models: Male Wistar rats of 150 g, high-fat diet (HFD) model[1]
Doses: 100 mg/kg/day Route of Administration: In the drinking water, 6 weeks Experimental Results: Dramatically prevented the rise in body weight in HFD rats, but not in animals that were fed a standard diet. decreased the increase in the weight of white adipose tissue (both epididymal and lumbar) in obese animals and attenuated their enhanced adiposity. Improved fasted glucose and insulin levels and consequently decreased HOMA index in the HFD group. Improved insulin signaling in adipose tissue from obese animals. Animal/Disease Models: C57BL/6 mice[3] Doses: 1 g/kg/day Route of Administration: In the drinking water, 4 weeks Experimental Results: Induce thoracic aortic dissection (TAD) in all mice with 24 h of Ang II infusion. Caused 87% of C57BL/6 mice to develop TAD without Ang II. |
| References |
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| Molecular Formula |
C3H6N2.HCL
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|---|---|
| Molecular Weight |
106.55408
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| Exact Mass |
106.03
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| CAS # |
646-03-7
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| Related CAS # |
β-Aminopropionitrile; 151-18-8
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| PubChem CID |
12244473
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| Appearance |
White to light yellow solid
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| Source |
Endogenously produced metabolite
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| LogP |
1.361
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
6
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| Complexity |
49.2
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(CN)C#N.Cl
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| InChi Key |
SOWHACJNMKTTRC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C3H6N2.ClH/c4-2-1-3-5;/h1-2,4H2;1H
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| Chemical Name |
3-aminopropanenitrile;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~938.5 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (23.46 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (23.46 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (23.46 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.3853 mL | 46.9263 mL | 93.8527 mL | |
| 5 mM | 1.8771 mL | 9.3853 mL | 18.7705 mL | |
| 10 mM | 0.9385 mL | 4.6926 mL | 9.3853 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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