| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
In an in vitro insulin resistance model, β-Aminopropionitrile (BAPN) increases glucose uptake and normalizes the expression of GLUT4 and adiponectin[1]. β-Aminopropionitrile (500 μM; 72 h) suppresses cervical cancer cells' ability to invade and migrate in vitro, as well as the morphological and marker protein alterations brought on by hypoxia-induced EMT[2].
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|---|---|
| ln Vivo |
In rats with diet-induced obesity, β-Aminopropionitrile (BAPN) (100 mg/kg/day; po; 6 weeks) improves the metabolic profile and decreases body weight gain[1]. C57BL/6 mice are given β-Aminopropionitrile monofumarate (1 g/kg/day; po; 4 weeks) to induce thoracic aortic dissection[3].
|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: 3T3-L1 adipocytes Tested Concentrations: 200 μM with 1.15 nM and 2.87 nM TNFα Incubation Duration: 72 h Experimental Results: TNFα decreased expression of GLUT4 and adiponectin, and increased SOCS3 protein levels in these cells. And these effects were prevented. Cell Invasion Assay[2] Cell Types: HeLa and SiHa cells Tested Concentrations: 500 μM Incubation Duration: 72 h Experimental Results: Dramatically decreased hypoxia- elicited cell invasion in both cell models. Cell Migration Assay [2] Cell Types: HeLa and SiHa cells Tested Concentrations: 500 μM Incubation Duration: 72 h Experimental Results: diminished hypoxia-induced migration from 180 and 240% to 60 and 70% in HeLa and SiHa cells, respectively. Western Blot Analysis[2] Cell Types: HeLa and SiHa cells Tested Concentrations: 500 μM Incubation Duration: 72 h Experimental Results: Effectively prevented hypoxia-induced downregulation of E-cadherin and strongly inhibited hypoxia-induced upregulation of α-SMA and vimentin. |
| Animal Protocol |
Animal/Disease Models: Male Wistar rats of 150 g, high-fat diet (HFD) model[1]
Doses: 100 mg/kg/day Route of Administration: In the drinking water, 6 weeks Experimental Results: Dramatically prevented the rise in body weight in HFD rats, but not in animals that were fed a standard diet. decreased the increase in the weight of white adipose tissue (both epididymal and lumbar) in obese animals and attenuated their enhanced adiposity. Improved fasted glucose and insulin levels and consequently decreased HOMA index in the HFD group. Improved insulin signaling in adipose tissue from obese animals. Animal/Disease Models: C57BL/6 mice[3] Doses: 1 g/kg/day Route of Administration: In the drinking water, 4 weeks Experimental Results: Induce thoracic aortic dissection (TAD) in all mice with 24 h of Ang II infusion. Caused 87% of C57BL/6 mice to develop TAD without Ang II. |
| References |
|
| Molecular Formula |
C3H6N2.HCL
|
|---|---|
| Molecular Weight |
106.55408
|
| Exact Mass |
106.03
|
| CAS # |
646-03-7
|
| PubChem CID |
12244473
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
1.361
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
6
|
| Complexity |
49.2
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(CN)C#N.Cl
|
| InChi Key |
SOWHACJNMKTTRC-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C3H6N2.ClH/c4-2-1-3-5;/h1-2,4H2;1H
|
| Chemical Name |
3-aminopropanenitrile;hydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.3853 mL | 46.9263 mL | 93.8527 mL | |
| 5 mM | 1.8771 mL | 9.3853 mL | 18.7705 mL | |
| 10 mM | 0.9385 mL | 4.6926 mL | 9.3853 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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