| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| Other Sizes |
| ln Vitro |
As a probe, Benzenepentacarboxylic Acid itself does not exhibit pharmacological activity (e.g., anti-proliferative, enzymatic inhibition). Its function is to quantitatively detect the generation of HO• in chemical systems, specifically in hydrogen peroxide (H₂O₂) bleaching systems with or without activators (TAED, TBCC). [1]
The fluorescence intensity of the product HBA (excitation at 311 nm, emission at 435 nm) is directly proportional to the concentration of HO• generated in the reaction system. [1] |
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| Enzyme Assay |
The study does not involve traditional enzyme activity assays. The “assay” described is a chemical detection method for HO• using Benzenepentacarboxylic Acid as a probe. The procedure is as follows: A working solution containing Benzenepentacarboxylic Acid (400 μmol/L) is prepared. This solution is added to the bleaching reaction systems (e.g., H₂O₂ alone, H₂O₂/TAED, H₂O₂/TBCC) which also contain NaOH, and the activators TAED or TBCC at specified concentrations. The reaction is carried out at a set temperature (e.g., 70°C) for 60 minutes. After the reaction, the mixture is promptly cooled and quenched. An aliquot of the working solution is placed in a quartz cuvette, and the fluorescence intensity is measured at the maximum excitation wavelength of 311 nm (with emission monitored at 435 nm) using a spectrofluorometer. The measured fluorescence intensity characterizes the relative concentration of HO• generated during the bleaching process. [1]
The probe Benzenepentacarboxylic Acid was found to be stable and reproducible even at high temperatures (above 90°C). [1] |
| References | |
| Additional Infomation |
Benzenepentacarboxylic acid (BPA) is a novel fluorescent probe for hydroxyl radicals (HO•) with high specificity, sensitivity, accuracy and reproducibility, developed by the authors' laboratory. [1] Its detection mechanism is based on a specific addition reaction, namely, the addition of HO• with BPA to generate a single fluorescent product - hydroxybenzenepentacarboxylic acid (HBA). Compared with probes that generate multiple products, this single-product reaction simplifies quantitative analysis and improves accuracy. [1] In this study, BPA was successfully used to validate and quantify the generation of HO• in the H₂O₂/TAED and H₂O₂/TBCC systems in cotton bleaching systems. The results showed that at 70℃, the amount of HO• generated in the H₂O₂/TAED and H₂O₂/TBCC systems was about 11 times and 15 times higher, respectively, than that in the pure H₂O₂ system under the same alkaline conditions. [1]
The application of this probe provides new insights into the bleaching mechanism, indicating that in addition to the traditionally recognized over-acid pathway, HO• generated by over-acid decomposition also plays an important role in oxidizing natural pigments and improving fabric whiteness, while also leading to a decrease in fabric strength. [1] |
| Molecular Formula |
C11H6O10
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|---|---|
| Molecular Weight |
298.1593
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| Exact Mass |
297.996
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| CAS # |
1585-40-6
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| PubChem CID |
15314
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| Appearance |
White to off-white solid powder
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| Density |
1.96g/cm3
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| Boiling Point |
642.3ºC at 760mmHg
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| Flash Point |
356.3ºC
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| Vapour Pressure |
2.24E-17mmHg at 25°C
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| Index of Refraction |
1.733
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| LogP |
0.177
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
21
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| Complexity |
471
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
QNSOHXTZPUMONC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H6O10/c12-7(13)2-1-3(8(14)15)5(10(18)19)6(11(20)21)4(2)9(16)17/h1H,(H,12,13)(H,14,15)(H,16,17)(H,18,19)(H,20,21)
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| Chemical Name |
benzene-1,2,3,4,5-pentacarboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 48 mg/mL (~160.99 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3539 mL | 16.7695 mL | 33.5390 mL | |
| 5 mM | 0.6708 mL | 3.3539 mL | 6.7078 mL | |
| 10 mM | 0.3354 mL | 1.6770 mL | 3.3539 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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