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| 5mg |
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| Targets |
BDP FL azide is a fluorescent labeling reagent rather than a compound with a traditional pharmacological target. The azide group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) Click Chemistry reactions with alkyne-functionalized biomolecules. Once conjugated to target molecules, the BDP FL fluorophore provides green fluorescence for detection and imaging applications. The dye has a high quantum yield in aqueous environments and excellent photostability, making it suitable for fluorescence-based assays and microscopy.
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| ln Vitro |
In vitro, BDP FL azide is used for labeling biomolecules via Click Chemistry. It can be conjugated to alkyne-modified proteins, nucleic acids, or lipids for fluorescence-based detection in various applications, including cell imaging, protein labeling, and fluorescence-based assays. The dye's green fluorescence is compatible with FAM fluorescence measurement instruments, allowing for multiplexed detection in combination with other fluorescent probes.
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| ln Vivo |
In vivo, BDP FL azide can be used for labeling of alkyne-functionalized biomolecules in live cells or organisms through metabolic labeling or bioorthogonal labeling strategies. The azide-alkyne Click Chemistry reaction is bioorthogonal and can be performed under physiological conditions, enabling the labeling and tracking of biomolecules in vivo. However, the compound itself is not a therapeutic agent but rather a labeling tool for studying biological processes in research settings.
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| Enzyme Assay |
For Click Chemistry labeling, the target molecule (alkyne-modified protein, nucleic acid, or lipid) is incubated with BDP FL azide in the presence of a copper catalyst (such as CuSO4 and sodium ascorbate) in an appropriate buffer. The reaction is typically carried out at room temperature for 1-24 hours. Labeled biomolecules can be purified by gel filtration, dialysis, or precipitation, and analyzed by SDS-PAGE, fluorescence spectroscopy, or mass spectrometry.
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| Cell Assay |
For cell labeling experiments, cells are incubated with alkyne-modified metabolic precursors (such as alkyne-modified amino acids, sugars, or nucleotides) to incorporate the alkyne functionality into newly synthesized biomolecules. After fixation and permeabilization, cells are incubated with BDP FL azide and copper catalyst for Click Chemistry labeling. Labeled cells are then imaged by fluorescence microscopy or analyzed by flow cytometry to visualize the distribution of the target biomolecules.
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| Animal Protocol |
For in vivo labeling studies, animals can be administered alkyne-modified metabolic precursors, followed by injection of BDP FL azide and copper catalyst for in vivo Click Chemistry labeling. Alternatively, tissues from animals that have been administered alkyne-modified precursors can be processed for ex vivo labeling with BDP FL azide. Labeled tissues can be sectioned and imaged by fluorescence microscopy to study biomolecule distribution and dynamics.
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| ADME/Pharmacokinetics |
BDP FL azide is soluble in DMSO and other organic solvents. The compound should be protected from light during handling and storage to prevent photobleaching. Storage is recommended at -20°C for long-term stability. The compound is for research use only and is not intended for human or veterinary use. Standard laboratory safety precautions should be followed when handling this compound.
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| Toxicity/Toxicokinetics |
Toxicological data for BDP FL azide have not been extensively reported, as it is a fluorescent labeling reagent rather than a therapeutic agent. Standard laboratory safety precautions should be followed when handling this compound. It is not intended for human use and is available for research purposes only.
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| References | |
| Additional Infomation |
BDP FL azide is a BDP dye linker with an azide group for Click Chemistry. It is a green fluorophore with high quantum yield in aqueous environments, high photostability, and FAM compatibility. The compound is used for labeling biomolecules including proteins, nucleic acids, and lipids. BDP FL azide is a research tool for fluorescence-based detection and imaging and is not approved for clinical use.
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| Molecular Formula |
C17H21BF2N6O
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|---|---|
| Molecular Weight |
374.196049451828
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| Exact Mass |
374.183
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| CAS # |
1379771-95-5
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| Related CAS # |
2243566-18-7;
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| PubChem CID |
91757873
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| Appearance |
Orange to red solid powder
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
27
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| Complexity |
771
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[B-]1(N2C(=CC=C2CCC(=O)NCCCN=[N+]=[N-])C=C3[N+]1=C(C=C3C)C)(F)F
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| InChi Key |
IKOHTCNDGJLUHG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H21BF2N6O/c1-12-10-13(2)25-16(12)11-15-5-4-14(26(15)18(25,19)20)6-7-17(27)22-8-3-9-23-24-21/h4-5,10-11H,3,6-9H2,1-2H3,(H,22,27)
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| Chemical Name |
N-(3-azidopropyl)-3-(2,2-difluoro-10,12-dimethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl)propanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6724 mL | 13.3618 mL | 26.7237 mL | |
| 5 mM | 0.5345 mL | 2.6724 mL | 5.3447 mL | |
| 10 mM | 0.2672 mL | 1.3362 mL | 2.6724 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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