| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Primary amine groups on proteins, peptides, and other amine-containing molecules. The BDP-581/591 dye also reacts with ROS, particularly lipid hydroperoxides and peroxyl radicals, making it useful for detecting oxidative stress.
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|---|---|
| ln Vitro |
BDP-581/591 NHS ester exhibits absorption at 585 nm and emission at 594 nm (Ex=585 nm, Em=594 nm). It has a relatively long fluorescence lifetime (3-5 ns) and a two-photon excitation cross-section for deep-tissue imaging. The dye's fluorescence changes upon reaction with ROS.
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| ln Vivo |
Not applicable; this is a labeling reagent for research use. Fluorescently labeled proteins conjugated with BDP-581/591 can be used for in vivo imaging of inflammation, cancer, or oxidative stress when administered to animals.
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| Enzyme Assay |
The NHS ester (1-10 mM in anhydrous DMSO or DMF) is added to protein (1-10 mg/mL) in labeling buffer (0.1 M sodium bicarbonate, pH 8.3-8.5) at 10-50:1 molar ratio (dye:protein). Incubate at room temperature for 1-2 hours. Free dye is removed by desalting or dialysis.
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| Cell Assay |
Labeled proteins (0.1-10 microg/mL) are added to live cells for 30-60 minutes at 37degC. Intracellular ROS levels can be measured by monitoring fluorescence changes. Alternatively, cells are treated with H2O2 or other ROS inducers, and fluorescence is measured on a plate reader (λex=585 nm, λem=594 nm).
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| Animal Protocol |
Not applicable for free NHS ester. BDP-581/591-labeled Annexin V or other apoptosis markers are injected intravenously (50-200 microg per mouse) to detect ROS-related cell death in disease models (e.g., ischemia-reperfusion injury, inflammation). Imaging is performed 1-4 hours post-injection.
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| ADME/Pharmacokinetics |
Not applicable for free NHS ester. The BDP-581/591 dye has excellent photostability and serum stability. Labeled proteins have half-lives of 6 hours to 10 days depending on the protein and conjugation site. The dye is non-toxic at labeling concentrations.
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| Toxicity/Toxicokinetics |
No toxicity; BDP-581/591 NHS ester is used at low concentrations for labeling purposes and has no known acute toxicity. The dye is stable under normal handling conditions. Avoid prolonged light exposure to prevent photobleaching.
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| Additional Infomation |
BDP-581/591 NHS ester is for research use only, not FDA-approved. It is widely used for ROS detection in live-cell imaging, flow cytometry, and two-photon microscopy. It is also used for fluorescence polarization analysis and protein-protein interaction studies.
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| Exact Mass |
489.167
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|---|---|
| CAS # |
654651-21-5
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| PubChem CID |
131954337
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| Appearance |
Brown to black solid powder
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| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
36
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| Complexity |
1070
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
[F-][B+3]1(N2=CC(/C=C/C=C/C3C=CC=CC=3)=CC2=CC2=CC(CCC(=O)ON3C(CCC3=O)=O)=C[N-]12)[F-]
|
| InChi Key |
HHJAMTPLXKVOAY-KBXRYBNXSA-N
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| InChi Code |
InChI=1S/C26H22BF2N3O4/c28-27(29)30-20(9-5-4-8-19-6-2-1-3-7-19)10-12-22(30)18-23-13-11-21(31(23)27)14-17-26(35)36-32-24(33)15-16-25(32)34/h1-13,18H,14-17H2/b8-4+,9-5+
|
| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-[2,2-difluoro-12-[(1E,3E)-4-phenylbuta-1,3-dienyl]-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl]propanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMF : 50 mg/mL (~102.19 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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