| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Azimsulfuron targets the enzyme acetolactate synthase (ALS), also known as acetohydroxyacid synthase (AHAS), which is a key enzyme in the biosynthesis of branched-chain amino acids (valine, leucine, and isoleucine) in plants. By inhibiting ALS, azimsulfuron disrupts protein synthesis and plant growth, leading to the death of susceptible weeds.
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| ln Vitro |
In vitro, azimsulfuron inhibits the activity of acetolactate synthase (ALS) enzyme isolated from plants. This is measured by the reduction in the formation of acetolactate from pyruvate. The herbicide's potency is determined by its IC50 value for ALS inhibition. Its selective toxicity is due to differential metabolism in crops versus weeds.
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| ln Vivo |
In vivo, azimsulfuron is applied to the foliage or soil of rice paddies and other crops. It is absorbed by the roots and foliage of weeds and translocated throughout the plant. It causes chlorosis and necrosis, effectively controlling a variety of broad-leaved weeds and sedges without damaging the rice crop when used at recommended rates.
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| Enzyme Assay |
The in vitro enzyme assay for azimsulfuron involves measuring its ability to inhibit acetolactate synthase (ALS). The enzyme is extracted from plant tissues and incubated with pyruvate in the presence of varying concentrations of the herbicide. The amount of acetolactate produced is measured colorimetrically, and the IC50 is determined.
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| Cell Assay |
In vitro cellular assays for azimsulfuron are not typically performed, as its site of action is at the enzymatic level within plants. However, its effects on plant cell cultures can be studied by measuring the inhibition of growth or the reduction in branched-chain amino acid levels.
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| Animal Protocol |
In vivo animal experiments for azimsulfuron are not typical, as it is an agrochemical. However, environmental toxicology studies are conducted to assess its safety to mammals, birds, fish, and other non-target organisms. These studies evaluate its acute and chronic toxicity, bioaccumulation, and effects on reproduction and development.
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| ADME/Pharmacokinetics |
Azimsulfuron is rapidly absorbed and metabolized in mammals, with low bioaccumulation potential. Its half-life in soil is typically short, ranging from days to weeks depending on environmental conditions. The herbicide is primarily excreted in urine.
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| Toxicity/Toxicokinetics |
Azimsulfuron has low acute oral toxicity to mammals (LD50 > 2000 mg/kg in rats). It is not a skin sensitizer or a mutagen. However, it can be irritating to the eyes and skin. It is classified as slightly toxic to birds and moderately toxic to fish. It has a low potential for bioaccumulation.
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| References | |
| Additional Infomation |
Azisulfuron is an N-sulfonylurea compound with a urea-like structure, in which one nitrogen atom is replaced by a 4,6-diamyrimidin-2-yl group, and the hydrogen atom on the other nitrogen atom is replaced by a [1-methyl-4-(2-methyl-2H-tetrazole-5-yl)-1H-pyrazol-5-yl]sulfonyl group. It is an acetolactate synthase inhibitor used as a herbicide to control various broadleaf and sedge weeds in paddy fields and other aquatic environments. It is both a herbicide and an EC 2.2.1.6 (acetolactate synthase) inhibitor. It is also an aromatic ether, biaryl compound, N-sulfonylurea compound, tetrazolium compound, and pyrazole insecticide.
Azimsulfuron is a widely used herbicide for weed control in rice paddies. It is marketed under various brand names. It was developed by DuPont and has been used since the 1980s. Its high efficacy and low toxicity have made it a valuable tool in rice production. It is not a pharmaceutical drug and has no human clinical applications. |
| Molecular Formula |
C13H16N10O5S
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|---|---|
| Molecular Weight |
424.40
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| Exact Mass |
424.102
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| CAS # |
120162-55-2
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| PubChem CID |
86355
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| Appearance |
White to off-white solid powder
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| Density |
1.7±0.1 g/cm3
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| Melting Point |
170 °C
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| Index of Refraction |
1.760
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| LogP |
-0.06
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
29
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| Complexity |
665
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
MAHPNPYYQAIOJN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H16N10O5S/c1-22-11(7(6-14-22)10-18-21-23(2)19-10)29(25,26)20-13(24)17-12-15-8(27-3)5-9(16-12)28-4/h5-6H,1-4H3,(H2,15,16,17,20,24)
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| Chemical Name |
1-(4,6-dimethoxypyrimidin-2-yl)-3-[2-methyl-4-(2-methyltetrazol-5-yl)pyrazol-3-yl]sulfonylurea
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3563 mL | 11.7813 mL | 23.5627 mL | |
| 5 mM | 0.4713 mL | 2.3563 mL | 4.7125 mL | |
| 10 mM | 0.2356 mL | 1.1781 mL | 2.3563 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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