| Size | Price | Stock | Qty |
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Purity: ≥98%
Azido-PEG2-alcohol is a PEG analogue containing an azide group and a terminal hydroxyl group. The hydrophilic PEG spacer increases solubility in aqueous media. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The hydroxyl group enables further derivatization or replacement with other reactive functional groups.
| Targets |
Azido-PEG2-alcohol is a PEG-based PROTAC linker that joins two essential ligands crucial for forming PROTAC molecules—one ligand binds to an E3 ubiquitin ligase and the other to the target protein—thereby enabling selective protein degradation through the intracellular ubiquitin-proteasome system [13†L3-L9] [14†L20-L24].
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| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
As a chemical linker/reagent, this compound is not itself a pharmacological agent and thus possesses no direct in vitro biological activity. It serves as a conjugation tool to construct active molecules rather than directly modulating biological functions. |
| ln Vivo |
As a chemical linker/reagent, this compound is not a pharmacological agent and thus lacks direct in vivo biological activity. It serves as a conjugation tool for constructing active molecules.
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| Enzyme Assay |
As a synthetic linker, this compound is typically characterized by chemical methods: purity is determined by HPLC (≥98%), structure is confirmed by 1H NMR and ESI-MS, and storage is recommended at 2-8°C for the compound itself; note that this is not a receptor-binding compound, so no such assay applies.
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| Cell Assay |
As a chemical linker, there is no standard cell-based assay protocol for the compound itself, as it is used exclusively as a synthetic intermediate. Storage conditions should be followed according to supplier guidelines.
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| Animal Protocol |
For in vivo use as part of a formulated product, typical vehicle formulations include DMSO : Tween 80 : Saline = 10 : 5 : 85, with working concentrations adjusted according to specific experimental requirements (e.g., IV/IP administration) [10†L33-L40].
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| ADME/Pharmacokinetics |
The compound is moderately soluble in aqueous media (up to ~100 mg/mL in water) due to the hydrophilic PEG spacer [10†L32-L33]. As a synthetic reagent, no independent ADME studies are available.
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| Toxicity/Toxicokinetics |
According to safety data sheets, this compound is classified with hazard statements: H315 (causes skin irritation), H319 (causes serious eye irritation), H335 (may cause respiratory irritation), and is harmful if swallowed; it is also very toxic to aquatic life with long-lasting effects (H410) [3†L5-L8] [17†L8-L9]. Precautions include wearing protective gloves/clothing and avoiding inhalation or contact with eyes and skin.
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| References |
| Molecular Formula |
C4H9N3O2
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|---|---|
| Molecular Weight |
131.133160352707
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| Exact Mass |
131.069
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| CAS # |
139115-90-5
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| Related CAS # |
139115-90-5;
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| PubChem CID |
11400789
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| Appearance |
Colorless to light yellow liquid
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| LogP |
0.3
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
9
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| Complexity |
100
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(COCCO)N=[N+]=[N-]
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| InChi Key |
CGJFKQFYZOARRV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C4H9N3O2/c5-7-6-1-3-9-4-2-8/h8H,1-4H2
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| Chemical Name |
2-(2-azidoethoxy)ethanol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~762.60 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.6260 mL | 38.1301 mL | 76.2602 mL | |
| 5 mM | 1.5252 mL | 7.6260 mL | 15.2520 mL | |
| 10 mM | 0.7626 mL | 3.8130 mL | 7.6260 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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