| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
Avenanthramide C targets oxidative stress and inflammatory pathways. It acts as a potent antioxidant, scavenging free radicals and inhibiting LDL oxidation. It also has anti-inflammatory activity, inhibiting the production of pro-inflammatory cytokines and adhesion molecules. Its mechanisms of action are related to its phenolic structure, which allows it to neutralize reactive oxygen species and modulate signaling pathways.
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| ln Vitro |
In vitro, avenanthramide C demonstrates potent antioxidant activity, including inhibition of LDL oxidation and scavenging of free radicals. It also exhibits anti-inflammatory activity, inhibiting the production of pro-inflammatory mediators such as TNF-α, IL-6, and IL-8 in cell-based assays. It has been shown to inhibit the expression of adhesion molecules such as VCAM-1 and ICAM-1.
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| ln Vivo |
In vivo, avenanthramide C has been studied for its anti-atherosclerotic effects. It has been shown to reduce the development of atherosclerosis in animal models. Its antioxidant and anti-inflammatory activities contribute to its cardiovascular protective effects. Avenanthramide C is absorbed from the diet and can be detected in plasma.
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| Enzyme Assay |
The activity of avenanthramide C can be assessed using cell-free assays to measure its antioxidant properties. The DPPH radical scavenging assay, ABTS radical cation decolorization assay, and inhibition of LDL oxidation assays can be used. Its anti-inflammatory activity can be assessed by measuring the inhibition of inflammatory mediators such as NO, TNF-α, and IL-6 in cell-free systems.
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| Cell Assay |
To evaluate the cellular effects of avenanthramide C, various cell lines (e.g., endothelial cells, macrophages) are treated with the compound. The inhibition of oxidative stress and inflammatory cytokine production is measured. The expression of adhesion molecules (VCAM-1, ICAM-1) is assessed by flow cytometry or Western blotting. Its cytoprotective effects are evaluated by measuring cell viability under oxidative stress.
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| Animal Protocol |
In vivo studies with avenanthramide C typically involve administration to animal models via oral routes, often as part of the diet. In models of atherosclerosis, the compound's effects on plaque formation, lipid profiles, and inflammatory markers are assessed. Its bioavailability and metabolism are also studied.
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| ADME/Pharmacokinetics |
Avenanthramide C has a molecular formula and weight that are not specified in the available literature. Its CAS number is 116764-15-9. It is a natural product found in oats. It is soluble in organic solvents. The purity is typically >98%. It should be stored according to the manufacturer's instructions.
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| Toxicity/Toxicokinetics |
Specific toxicology data for avenanthramide C are not extensively detailed in the available literature. As a natural compound found in food, it is generally considered safe at dietary intake levels. However, high doses may have adverse effects. As with all research compounds, standard safety precautions should be taken when handling avenanthramide C.
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| References |
Description: Avenanthramide C inhibits vascular smooth muscle cell proliferation and enhances nitric oxide production. |
| Additional Infomation |
2-{[(2E)-3-(3,4-dihydroxyphenyl)-1-hydroxypropyl-2-ene-1-yl]amino}-5-hydroxybenzoic acid is an alkaloid belonging to the benzamide class of compounds. Oat amide C has been reported in oats, and relevant data are available for reference.
Avenanthramide C is a natural phenolic compound found in oats (Avena sativa). It is a member of the avenanthramide family and has potent antioxidant, anti-inflammatory, and anti-atherosclerotic activities. Avenanthramide C is studied for its potential health benefits, including cardiovascular protection. It is a research tool for studying oxidative stress, inflammation, and atherosclerosis. |
| Molecular Formula |
C16H13NO6
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|---|---|
| Molecular Weight |
315.278
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| Exact Mass |
315.074
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| CAS # |
116764-15-9
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| Related CAS # |
116764-15-9;
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| PubChem CID |
11723200
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| Appearance |
Yellow to brown solid powder
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| Boiling Point |
702.3±60.0 °C(Predicted)
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| Melting Point |
248-250 °C
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| LogP |
2.803
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
466
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC(=C(C=C1C=CC(=O)NC2=C(C=C(C=C2)O)C(=O)O)O)O
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| InChi Key |
IDUUXROOZBOOPH-QHHAFSJGSA-N
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| InChi Code |
InChI=1S/C16H13NO6/c18-10-3-4-12(11(8-10)16(22)23)17-15(21)6-2-9-1-5-13(19)14(20)7-9/h1-8,18-20H,(H,17,21)(H,22,23)/b6-2+
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| Chemical Name |
2-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]amino]-5-hydroxybenzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1718 mL | 15.8589 mL | 31.7178 mL | |
| 5 mM | 0.6344 mL | 3.1718 mL | 6.3436 mL | |
| 10 mM | 0.3172 mL | 1.5859 mL | 3.1718 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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