| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Aspartame acesulfame does not have a traditional biological target like a receptor or enzyme. Its function is to bind to the sweet taste receptors (T1R2/T1R3) on the tongue, eliciting the perception of sweetness. Its components, aspartame and acesulfame, are both sweeteners that act on these same receptors. As a salt, it is designed to be stable and have a synergistic sweetening effect.
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| ln Vitro |
Aspartame is made up of methanol, aspartate, an excitatory neurotransmitter in the central nervous system, and phenylalanine, which is vital for neurotransmitter modulation [2].
Aspartame acesulfame demonstrates in vitro activity by activating sweet taste receptors. In cell-based assays using cells expressing the human T1R2/T1R3 receptor, the compound would stimulate a signaling cascade, typically measured by an increase in intracellular calcium or cAMP. Its potency is reflected in its sweetness intensity, which is 350 times that of sucrose. |
| ln Vivo |
Aspartame (4000 mg/kg bw/day; oral) did not cause any negative effects in mice, rats, hamsters, or dogs in acute, subacute, or long-term toxicity investigations of aspartame and its breakdown products[1].
In vivo, Aspartame acesulfame acts as a sweetener. When consumed, it is tasted and perceived as sweet, but it provides no significant calories. It is metabolized by the body into its constituent parts: phenylalanine, aspartic acid, and methanol. Its in vivo effect is purely sensory and nutritional, providing sweetness without caloric content. |
| Enzyme Assay |
There is no traditional enzyme/receptor binding assay for aspartame acesulfame as a drug, but an in vitro assay for its sweetening activity would involve a cell-based assay using the sweet taste receptor. Cells expressing the human T1R2/T1R3 receptor are treated with varying concentrations of aspartame acesulfame. The activation of the receptor is measured by a fluorescent calcium indicator or a reporter gene system. This assay is used to quantify its potency as a sweetener.
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| Cell Assay |
In vitro cellular assays for aspartame acesulfame are not typical for cytotoxicity studies, as it is a food additive. However, its safety is often assessed using standard toxicological assays, such as the Ames test for mutagenicity or cell viability assays on human cell lines. These assays would involve treating cells with high concentrations of the compound and assessing for DNA damage or cell death.
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| Animal Protocol |
In vivo animal experiments for aspartame acesulfame are typically conducted for safety and toxicological assessment, not for efficacy. These are long-term studies in rodents (rats and mice) where the compound is administered in the diet at various doses. Parameters monitored include body weight, food consumption, clinical signs, and histopathology of organs at the end of the study. These studies are used to determine the acceptable daily intake (ADI) for humans.
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| References |
| Molecular Formula |
C14H18N2O5.C4H5NO4S
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|---|---|
| Molecular Weight |
457.45492
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| Exact Mass |
262.095
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| CAS # |
106372-55-8
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| Related CAS # |
Aspartame;22839-47-0
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| PubChem CID |
25130065
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
0.238
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
31
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| Complexity |
662
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| Defined Atom Stereocenter Count |
2
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| SMILES |
OC(C[C@H](C(N[C@H](CC1C=CC=CC=1)C(OC)=O)=O)N)=O.CC1=CC(=O)NS(=O)(=O)O1
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| InChi Key |
KVHQNWGLVVERFR-ACMTZBLWSA-N
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| InChi Code |
InChI=1S/C14H18N2O5.C4H5NO4S/c1-21-14(20)11(7-9-5-3-2-4-6-9)16-13(19)10(15)8-12(17)18;1-3-2-4(6)5-10(7,8)9-3/h2-6,10-11H,7-8,15H2,1H3,(H,16,19)(H,17,18);2H,1H3,(H,5,6)/t10-,11-;/m0./s1
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| Chemical Name |
(3S)-3-amino-4-[[(2S)-1-methoxy-1-oxo-3-phenylpropan-2-yl]amino]-4-oxobutanoic acid;6-methyl-2,2-dioxooxathiazin-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1860 mL | 10.9302 mL | 21.8603 mL | |
| 5 mM | 0.4372 mL | 2.1860 mL | 4.3721 mL | |
| 10 mM | 0.2186 mL | 1.0930 mL | 2.1860 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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