| Size | Price | Stock | Qty |
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| Targets |
ANO1-IN-1 targets anoctamin-1 (ANO1/TMEM16A), a calcium-activated chloride channel that plays a role in epithelial ion transport, smooth muscle contraction, neuronal excitability, and cell proliferation. ANO1 is overexpressed in various cancers and is implicated in tumor growth and metastasis. By inhibiting ANO1, the compound may reduce cell proliferation and modulate ion transport.
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| ln Vitro |
ANO1-IN-1 (compound 9c) at 10 μM strongly prevents U251 cells from migrating and invading [1]. ANO1-IN-1 and TMZ work synergistically to significantly suppress the growth of GBM cells [1].
In vitro, ANO1-IN-1 is an inhibitor of ANO1 chloride channel activity. It shows inhibitory activity against the channel, with potential applications in cancer and hypertension research. However, specific IC50 values and detailed in vitro activity data are not extensively reported in the available literature. The compound is used as a research tool for studying ANO1 function. |
| ln Vivo |
In vivo, ANO1-IN-1 is expected to show efficacy in animal models of cancer, hypertension, and gastrointestinal disorders by inhibiting ANO1 activity. However, specific in vivo efficacy data and dosing regimens are not extensively reported in the available literature. The compound is primarily used as a research tool.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for ANO1-IN-1 typically involve patch-clamp electrophysiology or fluorescence-based membrane potential assays using cells expressing ANO1. Cells are voltage-clamped, and ANO1 currents are elicited by voltage steps in the presence of intracellular Ca2+. The compound (1-100 μM) is applied extracellularly, and current inhibition is measured. For fluorescence-based assays, cells are loaded with a membrane potential-sensitive dye, and changes in fluorescence are measured upon ANO1 activation.
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| Cell Assay |
In vitro cellular assays for ANO1-IN-1 use cell lines expressing ANO1 (e.g., cancer cell lines, epithelial cells). Cells are cultured in appropriate media and treated with various concentrations of the compound (0.001-10 μM) for 1-24 hours. ANO1 activity is assessed by measuring chloride efflux or membrane potential changes. Cell proliferation is assessed using MTT assays. Cell migration and invasion can be assessed using transwell assays.
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| Animal Protocol |
In vivo animal studies with ANO1-IN-1 would typically use mouse models of cancer or hypertension. The compound would be administered orally or intraperitoneally at doses of 1-30 mg/kg. Tumor growth, blood pressure, or gastrointestinal motility would be assessed. However, specific protocols are not extensively reported.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of ANO1-IN-1 are not extensively reported. Specific PK parameters such as oral bioavailability, half-life, and tissue distribution are not detailed. The compound is expected to be metabolized by hepatic enzymes.
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| Toxicity/Toxicokinetics |
The toxicity profile of ANO1-IN-1 is not extensively reported. As a chloride channel inhibitor, potential toxicities may include effects on epithelial fluid secretion and smooth muscle function. The compound is for research use only and not for human therapeutic use.
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| References | |
| Additional Infomation |
ANO1-IN-1 (CAS 407587-01-3) is an inhibitor of anoctamin-1 (ANO1/TMEM16A), a calcium-activated chloride channel. It is used in research on cancer, hypertension, and gastrointestinal disorders. However, specific IC50 values and detailed activity data are not extensively reported. The compound is available for research purposes only.
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| Molecular Formula |
C18H28N2O2S
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|---|---|
| Molecular Weight |
336.492123603821
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| Exact Mass |
336.187
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| CAS # |
407587-01-3
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| PubChem CID |
3148535
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| Appearance |
White to off-white solid powder
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| LogP |
4.8
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
478
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C12CC(C(C)(C)C)CCC=1C(C(N)=O)=C(NC(=O)C(C)(C)C)S2
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| InChi Key |
QJDASIZKRQHFFS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H28N2O2S/c1-17(2,3)10-7-8-11-12(9-10)23-15(13(11)14(19)21)20-16(22)18(4,5)6/h10H,7-9H2,1-6H3,(H2,19,21)(H,20,22)
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| Chemical Name |
6-tert-butyl-2-(2,2-dimethylpropanoylamino)-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~742.96 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9719 mL | 14.8593 mL | 29.7186 mL | |
| 5 mM | 0.5944 mL | 2.9719 mL | 5.9437 mL | |
| 10 mM | 0.2972 mL | 1.4859 mL | 2.9719 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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