| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Alpha-Estradiol-d2 targets estrogen receptors (ERα and ERβ) as a weak estrogen and also inhibits 5α-reductase. By inhibiting 5α-reductase, Alpha-Estradiol reduces the conversion of testosterone to dihydrotestosterone (DHT), which is implicated in androgenic alopecia. The deuterium label increases the molecule's metabolic stability, making it valuable for pharmacokinetic and metabolic studies.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, Alpha-Estradiol-d2 is used as a tracer to study estrogen receptor binding and 5α-reductase inhibition. The compound does not exhibit intrinsic pharmacological activity beyond that of Alpha-Estradiol but serves as a quantitative tool for studying hormone metabolism and receptor interactions in cell-based systems. |
| ln Vivo |
In vivo, Alpha-Estradiol-d2 is used in pharmacokinetic and metabolic studies to trace the distribution and metabolism of Alpha-Estradiol. The deuterium label allows for precise quantification of the compound and its metabolites in plasma and tissues using mass spectrometry. The compound is used in research on androgenic alopecia and hormone-related conditions.
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| Enzyme Assay |
As an analytical standard, Alpha-Estradiol-d2 is used in steroid hormone analysis. Typical protocols involve spiking the labeled compound into samples prior to extraction and analysis by LC-MS/MS. The compound serves as an internal standard to correct for matrix effects and instrument variability. The labeled compound co-elutes with the unlabeled Alpha-Estradiol but is detected at a different mass-to-charge ratio.
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| Cell Assay |
In vitro cell culture experiments using Alpha-Estradiol-d2 involve treating hormone-responsive cells with the labeled compound and analyzing its uptake and metabolism by mass spectrometry. The compound can be used to study estrogen receptor signaling, 5α-reductase inhibition, and the effects of Alpha-Estradiol on cell proliferation and gene expression.
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| Animal Protocol |
In vivo animal studies using Alpha-Estradiol-d2 involve administering the compound to rodents via oral gavage, subcutaneous injection, or intramuscular injection. Blood samples are collected at various time points, and the concentration of the labeled compound and its metabolites is measured by mass spectrometry. Tissue distribution and metabolic profiling can also be performed.
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| ADME/Pharmacokinetics |
Alpha-Estradiol-d2 exhibits pharmacokinetic properties similar to those of unlabeled Alpha-Estradiol. As a steroid hormone, it is well-absorbed and distributed throughout the body. It is metabolized in the liver and excreted primarily in urine. The deuterium label provides a distinct mass shift for analytical detection without significantly altering the compound's physicochemical properties.
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| Toxicity/Toxicokinetics |
Alpha-Estradiol-d2 is considered safe for research use at typical concentrations. The compound is non-hazardous for transport. As a stable isotope-labeled compound, it is not intended for therapeutic use and is handled under standard laboratory safety practices.
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| References |
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| Additional Infomation |
Alpha-Estradiol-d2 (17α-Estradiol-2,4-d2) has a molecular formula of C18H22D2O2 and a molecular weight of 274.40. Isotopic enrichment is typically 98 atom% D. Purity is typically 98%. Alpha-Estradiol is a weak estrogen and 5α-reductase inhibitor with potential use in the study of androgenic alopecia.
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| Molecular Formula |
C18H24O2
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|---|---|
| Molecular Weight |
272.38196
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| Exact Mass |
274.19
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| CAS # |
81586-94-9
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| Related CAS # |
Alpha-Estradiol;57-91-0
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| PubChem CID |
90477423
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| Appearance |
White to off-white solid powder
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| LogP |
3.609
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
20
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| Complexity |
382
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| Defined Atom Stereocenter Count |
5
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| SMILES |
[2H]C1=CC2=C(CC[C@@H]3[C@@H]2CC[C@]4([C@H]3CC[C@H]4O)C)C(=C1O)[2H]
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| InChi Key |
VOXZDWNPVJITMN-BHOSFUFTSA-N
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| InChi Code |
InChI=1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17-,18+/m1/s1/i3D,10D
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| Chemical Name |
(8R,9S,13S,14S,17R)-2,4-dideuterio-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6713 mL | 18.3567 mL | 36.7134 mL | |
| 5 mM | 0.7343 mL | 3.6713 mL | 7.3427 mL | |
| 10 mM | 0.3671 mL | 1.8357 mL | 3.6713 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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