| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
α-Amyrin has multiple targets and biological activities. It activates the ERK and GSK-3β signaling pathways. It inhibits HIV-1 reverse transcriptase with an IC50 of 3.3 µM. The compound has long-lasting analgesic and anti-inflammatory properties. As a natural triterpenoid, it interacts with various cellular targets involved in inflammation, metabolism, and neuroprotection. Its diverse activities make it a subject of research for multiple disease indications.
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| ln Vitro |
In vitro, α-Amyrin has been shown to activate ERK and GSK-3β signaling pathways. It inhibits HIV-1 reverse transcriptase with an IC50 of 3.3 µM. The compound has demonstrated anti-inflammatory and analgesic activities in various cell-based assays. Its effects on cell signaling and enzyme activity support its potential for studying metabolic syndrome, cognitive dysfunction, and viral infections.
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| ln Vivo |
In vivo, α-Amyrin has oral activity and can be used in the study of metabolic syndrome induced by high fructose diet and cognitive dysfunction caused by low cholinergic neurotransmission. It has long-lasting analgesic and anti-inflammatory properties. The compound has been isolated from various plant sources and has diverse biological activities. Specific in vivo efficacy data in disease models would be detailed in the primary literature.
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| Enzyme Assay |
For enzyme inhibition assays, HIV-1 reverse transcriptase is incubated with a template-primer and nucleotides in assay buffer. α-Amyrin is added at various concentrations (typically 0.1-100 µM). Enzyme activity is measured by monitoring nucleotide incorporation, and IC50 values are calculated. For cell signaling studies, cells are treated with α-Amyrin, and phosphorylation of ERK and GSK-3β is assessed by Western blotting.
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| Cell Assay |
For cell-based assays, cells are treated with α-Amyrin at various concentrations for different time points. Cell viability, proliferation, and signaling pathway activation are assessed. Anti-inflammatory activity can be evaluated by measuring cytokine production in response to inflammatory stimuli. The compound's effects on metabolic parameters such as glucose uptake or lipid metabolism can also be assessed in relevant cell types.
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| Animal Protocol |
For in vivo studies, animal models of metabolic syndrome (such as high fructose diet-induced models) or cognitive dysfunction (such as models of low cholinergic neurotransmission) would be used. α-Amyrin would be administered orally at doses determined from pharmacokinetic studies. Metabolic parameters (blood glucose, lipids), cognitive function (behavioral tests), and inflammatory markers would be assessed to evaluate efficacy. However, specific protocols for α-Amyrin have not been detailed.
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| ADME/Pharmacokinetics |
α-Amyrin has oral activity. As a pentacyclic triterpenoid, it has favorable lipophilic properties for oral absorption. Its molecular weight of 426.72 g/mol is within the typical range for drug-like molecules. Further detailed PK parameters including half-life, bioavailability, and tissue distribution would be available in the primary literature. The compound is typically stored at -20°C for long-term stability.
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| Toxicity/Toxicokinetics |
Toxicological data for α-Amyrin have not been extensively reported in the available literature. As a natural product, it has been used in traditional medicine and is generally considered to have a favorable safety profile. However, comprehensive toxicology studies would be required for clinical development. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
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| Additional Infomation |
α-Amyrinol is a pentacyclic triterpenoid compound belonging to the arbutin type, with a double bond between positions 12 and 13, and a hydrogen atom at the 3β position replaced by a hydroxyl group. It is a pentacyclic triterpenoid and also a secondary alcohol. It is derived from the hydride of arbutin. α-Amyrinol has been reported to be found in tea tree (Camellia sinensis), elderberry (Sambucus chinensis), and other organisms with relevant data. See also: Calendula (partial); Viburnum bark (partial); Eupatorium perfoliatum whole plant (partial)... See more...
α-Amyrin is a pentacyclic triterpenoid compound with oral activity. It activates ERK and GSK-3β signaling pathways. α-Amyrin can be used in the study of metabolic syndrome and cognitive dysfunction. It inhibits HIV-1 reverse transcriptase with IC50 of 3.3 µM. The compound has long-lasting analgesic and anti-inflammatory properties. α-Amyrin is a research compound and is not approved for clinical use. |
| Molecular Formula |
C30H50O
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|---|---|
| Molecular Weight |
426.72
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| Exact Mass |
426.386
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| CAS # |
638-95-9
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| PubChem CID |
73170
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
493.8±45.0 °C at 760 mmHg
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| Melting Point |
178-183°C
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| Flash Point |
218.6±21.0 °C
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| Vapour Pressure |
0.0±2.8 mmHg at 25°C
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| Index of Refraction |
1.538
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| LogP |
11.01
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
31
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| Complexity |
779
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| Defined Atom Stereocenter Count |
10
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| SMILES |
C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)O)C)C)[C@@H]2[C@H]1C)C)C
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| InChi Key |
FSLPMRQHCOLESF-SFMCKYFRSA-N
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| InChi Code |
InChI=1S/C30H50O/c1-19-11-14-27(5)17-18-29(7)21(25(27)20(19)2)9-10-23-28(6)15-13-24(31)26(3,4)22(28)12-16-30(23,29)8/h9,19-20,22-25,31H,10-18H2,1-8H3/t19-,20+,22+,23-,24+,25+,27-,28+,29-,30-/m1/s1
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| Chemical Name |
(3S,4aR,6aR,6bS,8aR,11R,12S,12aR,14aR,14bR)-4,4,6a,6b,8a,11,12,14b-octamethyl-2,3,4a,5,6,7,8,9,10,11,12,12a,14,14a-tetradecahydro-1H-picen-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3435 mL | 11.7173 mL | 23.4346 mL | |
| 5 mM | 0.4687 mL | 2.3435 mL | 4.6869 mL | |
| 10 mM | 0.2343 mL | 1.1717 mL | 2.3435 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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