| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Alizarine Yellow R does not have a defined biological target as it is a dye rather than a drug. It is used as a pH indicator and as a dye for staining biological specimens. Its mechanism of action is physical, based on its color change properties in response to pH.
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|---|---|
| ln Vitro |
Alizarine Yellow R is not a bioactive compound with defined pharmacological activity. Its in vitro activity is related to its use as a pH indicator and a dye. The compound changes color from yellow to red in response to pH changes, making it useful for pH determination and biological staining.
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| ln Vivo |
Alizarine Yellow R is not used as a therapeutic agent and does not have defined in vivo pharmacological activity. Its applications are limited to laboratory and industrial settings as a pH indicator and dye. The compound is not intended for in vivo use as a drug.
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| Enzyme Assay |
In vitro assays for Alizarine Yellow R typically involve measuring its color change properties in response to pH. The compound is dissolved in appropriate buffers at various pH values, and UV-Vis spectra are recorded. The pH-dependent color change and absorption maxima are characterized.
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| Cell Assay |
Cell-based assays are not applicable for Alizarine Yellow R as it is not a bioactive compound. The compound is used as a dye for staining biological specimens in histology and microscopy. It is not intended for use in cellular assays or as a therapeutic agent.
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| Animal Protocol |
In vivo animal studies are not applicable for Alizarine Yellow R as it is not a therapeutic agent. The compound is used in laboratory and industrial applications as a pH indicator and dye. It is not administered to animals for pharmacological evaluation.
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| ADME/Pharmacokinetics |
Alizarine Yellow R has a molecular weight of 287.23 and a molecular formula of C13H9N3O5. It appears as a yellow to orange-red powder. The compound is soluble in water and organic solvents. It is used as a pH indicator with a transition range of pH 10.0-12.0 (yellow to red).
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| Toxicity/Toxicokinetics |
Alizarine Yellow R is generally considered safe for handling in laboratory settings when appropriate precautions are taken. The compound may cause irritation upon contact with skin or eyes. It is not intended for human therapeutic use and is available only for research and industrial purposes. Standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
Alizarine Yellow R is an azo dye used as a pH indicator and as a dye for textile and biological staining applications. It changes color from yellow to red in response to pH changes, with a transition range of pH 10.0-12.0. The compound is not a therapeutic agent and is intended for research and industrial use only.
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| Molecular Formula |
C13H9N3O5
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|---|---|
| Molecular Weight |
287.2277
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| Exact Mass |
287.054
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| CAS # |
2243-76-7
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| PubChem CID |
15588
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| Appearance |
Brown to dark brown solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
571.4±50.0 °C at 760 mmHg
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| Melting Point |
250°C
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| Flash Point |
299.4±30.1 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.672
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| LogP |
4.26
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
21
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| Complexity |
415
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YVJPMMYYRNHJAU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H9N3O5/c17-12-6-3-9(7-11(12)13(18)19)15-14-8-1-4-10(5-2-8)16(20)21/h1-7,17H,(H,18,19)
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| Chemical Name |
2-hydroxy-5-[(4-nitrophenyl)diazenyl]benzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~87.04 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4815 mL | 17.4077 mL | 34.8153 mL | |
| 5 mM | 0.6963 mL | 3.4815 mL | 6.9631 mL | |
| 10 mM | 0.3482 mL | 1.7408 mL | 3.4815 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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