| Size | Price | Stock | Qty |
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| 1mg |
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| 50mg | |||
| Other Sizes |
| Targets |
Agroclavine targets multiple receptors. It is a D1 dopamine receptor agonist, meaning it activates the D1 receptor, a G protein-coupled receptor that plays a key role in the central nervous system. Agroclavine is also an α1-adrenoceptor agonist, activating α1-adrenergic receptors. By binding to and activating these receptors, Agroclavine modulates various physiological processes, including neurotransmission, smooth muscle contraction, and blood pressure regulation. Its activity at these receptors is responsible for its pharmacological effects.
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| ln Vitro |
In vitro, Agroclavine has been shown to have agonist activity at D1 dopamine receptors and α1-adrenoceptors. Its activity is typically measured using receptor binding and functional assays. In functional assays, the compound's ability to activate D1 receptor-mediated signaling, such as cAMP accumulation, or α1-adrenoceptor-mediated signaling, such as calcium mobilization, is assessed. Agroclavine also exhibits anticancer and antimicrobial activities in vitro. However, specific in vitro data, such as IC50 or EC50 values, are not detailed in standard product descriptions.
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| ln Vivo |
In vivo activity data for Agroclavine is limited, as the compound is primarily used as a research tool in in vitro studies. As a D1 dopamine receptor and α1-adrenoceptor agonist, it has potential applications in studying these receptor systems in vivo. However, specific in vivo protocols and results are not detailed in standard product descriptions. Agroclavine is a research compound used to study receptor pharmacology and its potential therapeutic effects.
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| Enzyme Assay |
In vitro receptor binding assays for Agroclavine measure its affinity for D1 dopamine receptors and α1-adrenoceptors. Membranes from cells expressing these receptors are incubated with radiolabeled ligands and varying concentrations of Agroclavine. The Ki is determined from competition binding curves. Functional assays measure the activation of receptor-mediated signaling, such as cAMP accumulation for D1 receptors or calcium mobilization for α1-adrenoceptors. These assays confirm the compound's activity at these receptors.
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| Cell Assay |
In vitro cell-based assays for Agroclavine are used to study its effects on receptor signaling. Cells expressing D1 dopamine receptors or α1-adrenoceptors are treated with Agroclavine, and downstream signaling is measured. The compound's ability to activate these receptors is assessed. These assays confirm the compound's agonist activity at D1 and α1 receptors.
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| Animal Protocol |
In vivo animal experiments for Agroclavine are not extensively described in the available literature. As a research compound, its use in vivo would be determined by the specific research question being addressed. A typical protocol for studying a D1 receptor agonist would involve its administration to animal models of neurological or psychiatric disorders. However, specific protocols for Agroclavine are not detailed.
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| ADME/Pharmacokinetics |
Agroclavine has a molecular weight of 238.33 g/mol and a molecular formula of C16H18N2. It has a CAS number of 548-42-5. It is a solid compound. For storage, it is recommended to keep the powder at -20°C. Detailed pharmacokinetic properties such as absorption, distribution, metabolism, and excretion (ADME) have not been extensively characterized. As a research compound, its stability is maintained by proper storage.
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| Toxicity/Toxicokinetics |
Detailed toxicity data for Agroclavine is not provided in standard product descriptions. As a natural ergot alkaloid, it may have toxic effects, particularly at high doses. Ergot alkaloids are known to cause vasoconstriction and other adverse effects. As with all research chemicals, standard laboratory safety precautions should be followed when handling Agroclavine. Its use is limited to research applications and it is not intended for human or veterinary use.
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| References | |
| Additional Infomation |
Agroclavine is an ergot alkaloid belonging to the ergoline family. It has a double bond between positions 8 and 9, with methyl groups at positions 6 and 8. It is the conjugate base of Agroclavine (1+). Agroclavine has been reported in Epichloe typhina, Balansia strangulans, and other organisms with relevant data. Agroclavine is a naturally occurring ergoline alkaloid. It is named Agroclavine because it is derived from dimethylergoline. Like other ergoline alkaloids, it is found in various vines of the Convolvulaceae family (Ipomoea family) and some lower fungi. Prolonged exposure to certain ergoline alkaloids can lead to ergot poisoning, a disease characterized by convulsions and gangrene. (L1918)
Agroclavine is a research compound and is not approved for any clinical or therapeutic use. It is a natural clavine-type ergot alkaloid. Agroclavine is a D1 dopamine receptor and α1-adrenoceptor agonist. It also has anticancer and antimicrobial activities. Agroclavine is a valuable research tool for studying dopamine and adrenergic receptor pharmacology, as well as its potential therapeutic effects. Its mechanism of action involves activating D1 dopamine receptors and α1-adrenoceptors. |
| Molecular Formula |
C16H18N2
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|---|---|
| Molecular Weight |
238.33
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| Exact Mass |
238.147
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| CAS # |
548-42-5
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| PubChem CID |
73484
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.161g/cm3
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| Boiling Point |
422.5ºC at 760mmHg
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| Flash Point |
209.3ºC
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| Index of Refraction |
1.653
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| LogP |
3.005
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
18
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| Complexity |
375
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| Defined Atom Stereocenter Count |
2
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| SMILES |
CC1=CC2C(CC3=CNC4=CC=CC2=C34)N(C1)C
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| InChi Key |
XJOOMMHNYOJWCZ-UKRRQHHQSA-N
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| InChi Code |
InChI=1S/C16H18N2/c1-10-6-13-12-4-3-5-14-16(12)11(8-17-14)7-15(13)18(2)9-10/h3-6,8,13,15,17H,7,9H2,1-2H3/t13-,15-/m1/s1
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| Chemical Name |
(6aR,10aR)-7,9-dimethyl-6,6a,8,10a-tetrahydro-4H-indolo[4,3-fg]quinoline
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| Synonyms |
AGROCLAVIN Agroclavine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1959 mL | 20.9793 mL | 41.9586 mL | |
| 5 mM | 0.8392 mL | 4.1959 mL | 8.3917 mL | |
| 10 mM | 0.4196 mL | 2.0979 mL | 4.1959 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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