| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Agrimonolide targets multiple pathways and receptors. It is a potential α1 adrenergic receptor antagonist. It exerts anti-inflammatory activity by suppressing LPS-induced activation of JAK-STATs and p38 MAPKs signaling pathways. By inhibiting these signaling pathways, Agrimonolide reduces the production of pro-inflammatory cytokines. Its activity at the α1 adrenergic receptor suggests potential effects on cardiovascular and smooth muscle function.
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| ln Vitro |
In vitro, Agrimonolide has been shown to exert anti-inflammatory activity. It suppresses LPS-induced activation of JAK-STATs and p38 MAPKs signaling pathways. Its activity as a potential α1 adrenergic receptor antagonist has been suggested. However, specific in vitro data, such as IC50 or EC50 values, are not detailed in standard product descriptions. Agrimonolide is a naturally occurring compound used in research.
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| ln Vivo |
In vivo activity data for Agrimonolide is limited, as the compound is primarily used as a research tool in in vitro studies. Its anti-inflammatory properties suggest potential in vivo applications in inflammatory disease models. However, specific in vivo protocols and results are not detailed in standard product descriptions. Agrimonolide is a research compound used to study anti-inflammatory mechanisms and receptor pharmacology.
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| Enzyme Assay |
In vitro assays for Agrimonolide measure its anti-inflammatory activity and its effects on signaling pathways. In a typical assay, immune cells (e.g., macrophages) are treated with lipopolysaccharide (LPS) to induce inflammation, in the presence or absence of Agrimonolide. The production of pro-inflammatory cytokines (e.g., TNF-α, IL-6) is measured by ELISA. The activation of JAK-STATs and p38 MAPKs signaling pathways is assessed by Western blotting using phospho-specific antibodies.
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| Cell Assay |
In vitro cell-based assays for Agrimonolide are used to study its anti-inflammatory effects on immune cells. Macrophages or other immune cells are treated with LPS in the presence or absence of Agrimonolide. The production of pro-inflammatory cytokines is measured by ELISA. The activation of signaling pathways is assessed by Western blotting. These assays confirm the compound's anti-inflammatory activity and its effects on JAK-STAT and p38 MAPK signaling.
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| Animal Protocol |
In vivo animal experiments for Agrimonolide are not extensively described in the available literature. As a research compound, its use in vivo would be determined by the specific research question being addressed. A typical protocol for studying an anti-inflammatory compound would involve its administration to animal models of inflammation. However, specific protocols for Agrimonolide are not detailed.
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| ADME/Pharmacokinetics |
Agrimonolide has a molecular weight of 314.33 g/mol and a molecular formula of C18H18O5. It has a CAS number of 21499-24-1. It is a solid compound. It is soluble in organic solvents. For storage, it is recommended to keep the powder at -20°C. Detailed pharmacokinetic properties such as absorption, distribution, metabolism, and excretion (ADME) have not been extensively characterized. As a research compound, its stability is maintained by proper storage.
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| Toxicity/Toxicokinetics |
Detailed toxicity data for Agrimonolide is not provided in standard product descriptions. As a natural product, it is generally considered to have low toxicity. However, comprehensive toxicological studies have not been reported. As with all research chemicals, standard laboratory safety precautions should be followed when handling Agrimonolide. Its use is limited to research applications.
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| References | |
| Additional Infomation |
Reports indicate that Spiraea taiwanensis and Agrimonia pilosa contain agrimonolactone, and relevant data is available for reference.
Agrimonolide is a research compound and is not approved for any clinical or therapeutic use. It is a natural bioactive compound isolated from Agrimonia pilosa Ledeb. and Spiraea formosana Hayata. It is a derivative of isocoumarins. Agrimonolide is a potential α1 adrenergic receptor antagonist and exerts anti-inflammatory activity by suppressing LPS-induced activation of JAK-STATs and p38 MAPKs signaling pathways. It is a valuable research tool for studying anti-inflammatory mechanisms and receptor pharmacology. |
| Molecular Formula |
C18H18O5
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|---|---|
| Molecular Weight |
314.33252
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| Exact Mass |
314.115
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| CAS # |
21499-24-1
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| PubChem CID |
161362
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
581.1±50.0 °C at 760 mmHg
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| Flash Point |
215.5±23.6 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.612
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| LogP |
3.49
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
404
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| Defined Atom Stereocenter Count |
1
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| SMILES |
COC1=CC=C(C=C1)CC[C@H]2CC3=C(C(=CC(=C3)O)O)C(=O)O2
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| InChi Key |
TYFJTEPDESMEHE-HNNXBMFYSA-N
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| InChi Code |
InChI=1S/C18H18O5/c1-22-14-5-2-11(3-6-14)4-7-15-9-12-8-13(19)10-16(20)17(12)18(21)23-15/h2-3,5-6,8,10,15,19-20H,4,7,9H2,1H3/t15-/m0/s1
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| Chemical Name |
(3S)-6,8-dihydroxy-3-[2-(4-methoxyphenyl)ethyl]-3,4-dihydroisochromen-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1814 mL | 15.9068 mL | 31.8137 mL | |
| 5 mM | 0.6363 mL | 3.1814 mL | 6.3627 mL | |
| 10 mM | 0.3181 mL | 1.5907 mL | 3.1814 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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