| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Acetoxycycloheximide targets the protein synthesis machinery, specifically the ribosome, to inhibit translation. By inhibiting protein synthesis, it triggers apoptosis through the JNK pathway. It also modulates TNF receptor 1 levels, affecting TNF-α-mediated signaling.
|
|---|---|
| ln Vitro |
In vitro, Acetoxycycloheximide induces apoptosis by activating the JNK pathway and releasing cytochrome c from mitochondria. It also downregulates cell surface TNF-R1. Its activity is measured in cell viability assays, where it shows potent antitumor activity. It induces procaspase-3 activation.
|
| ln Vivo |
In vivo, Acetoxycycloheximide has been studied for its antitumor properties. Its mechanism of inducing apoptosis and inhibiting protein synthesis makes it a potential anticancer agent. Specific in vivo data are not detailed in the search results.
|
| Enzyme Assay |
The in vitro assay for Acetoxycycloheximide involves measuring its ability to inhibit protein synthesis, which can be assessed using a cell-free translation system. Its ability to induce apoptosis is measured by caspase activity assays or by assessing cytochrome c release from mitochondria.
|
| Cell Assay |
Cellular in vitro experiments for Acetoxycycloheximide are conducted using cancer cell lines. Cells are treated with the compound, and cell viability is assessed using MTT or similar assays. Apoptosis is measured using Annexin V staining or caspase activity assays. The activation of JNK and release of cytochrome c are assessed by Western blot.
|
| Animal Protocol |
In vivo animal experiments for Acetoxycycloheximide would involve using mouse xenograft models of cancer. The compound would be administered, and tumor growth would be monitored. Tumors would be harvested for analysis of apoptosis markers.
|
| References |
|
| Molecular Formula |
C17H25NO6
|
|---|---|
| Molecular Weight |
339.3835
|
| Exact Mass |
339.168
|
| CAS # |
2885-39-4
|
| PubChem CID |
233768
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.24g/cm3
|
| Boiling Point |
529.4ºC at 760mmHg
|
| Flash Point |
274ºC
|
| LogP |
1.056
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
24
|
| Complexity |
543
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(OC1(CC(C)C(=O)C(C(CC2CC(=O)NC(=O)C2)O)C1)C)=O
|
| InChi Key |
UFDHNJJHPSGMFX-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C17H25NO6/c1-9-7-17(3,24-10(2)19)8-12(16(9)23)13(20)4-11-5-14(21)18-15(22)6-11/h9,11-13,20H,4-8H2,1-3H3,(H,18,21,22)
|
| Chemical Name |
[3-[2-(2,6-dioxopiperidin-4-yl)-1-hydroxyethyl]-1,5-dimethyl-4-oxocyclohexyl] acetate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9465 mL | 14.7327 mL | 29.4655 mL | |
| 5 mM | 0.5893 mL | 2.9465 mL | 5.8931 mL | |
| 10 mM | 0.2947 mL | 1.4733 mL | 2.9465 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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