| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
Acetamiprid-d3 targets the same biological targets as unlabeled acetamiprid, a neonicotinoid insecticide that acts as an agonist of insect nicotinic acetylcholine receptors (nAChRs). nAChRs are ligand-gated ion channels that mediate fast synaptic transmission in the insect nervous system. By activating nAChRs, acetamiprid causes overstimulation of the insect nervous system, leading to paralysis and death. The deuterium-labeled compound is primarily used as an analytical standard for quantification studies.
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|---|---|
| ln Vitro |
Acetamiprid-d3 is a deuterium-labeled neonicotinoid insecticide used as an internal standard for analytical quantification. Acetamiprid, the unlabeled parent compound, is a neonicotinoid insecticide that acts as an agonist of insect nAChRs. It activates nAChRs containing specific subunits, leading to overstimulation of the insect nervous system. The labeled compound allows for the accurate quantification of acetamiprid residues in environmental and biological samples by mass spectrometry.
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| ln Vivo |
In vivo, acetamiprid (the unlabeled parent compound) is a neonicotinoid insecticide used to control a wide range of insect pests in agriculture. Acetamiprid-d3 is used as an internal standard for the quantification of acetamiprid residues in environmental and biological samples. The labeled compound can be administered to plants or animals as a tracer to study acetamiprid metabolism and distribution. Specific in vivo efficacy studies for the labeled compound are not applicable, as it is used as an analytical standard.
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| Enzyme Assay |
In vitro assays for Acetamiprid-d3 typically involve its use as an internal standard in mass spectrometry-based analytical methods. The compound is added to samples at known concentrations before extraction and analysis. Samples are analyzed by GC-MS or LC-MS/MS to quantify acetamiprid and its metabolites. Standard protocols involve extraction from matrices such as plant tissues, soil, or water, followed by purification and mass spectrometric analysis. The compound is dissolved in appropriate solvents such as acetonitrile or methanol.
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| Cell Assay |
In vitro cellular assays for Acetamiprid-d3 are not typical, as the compound is used as an analytical standard rather than for biological activity assessment. For studies of acetamiprid's insecticidal activity, insect cell lines or neurons are treated with unlabeled acetamiprid, and the labeled compound is used for quantification. The compound is dissolved in appropriate solvents and diluted for assay preparation. Cellular responses such as calcium influx or membrane depolarization can be measured to assess nAChR activation.
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| Animal Protocol |
In vivo animal studies for Acetamiprid-d3 are not typically performed for therapeutic purposes, as the compound is an insecticide rather than a therapeutic agent. The compound may be used in toxicological studies to assess acetamiprid metabolism and distribution in animals. The labeled compound is administered to animals via oral gavage or dietary exposure, and blood, urine, and tissue samples are collected for mass spectrometry analysis. The compound is not intended for human therapeutic use.
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| ADME/Pharmacokinetics |
Acetamiprid-d3 has a molecular weight of 225.69 g/mol and formula C10H8D3ClN4. It has a purity of 98%. The compound is intended for use as an internal standard for the quantification of acetamiprid by GC- or LC-MS. It is typically stored at -20°C. The compound is soluble in organic solvents such as acetonitrile and methanol. Detailed PK parameters are not applicable for this analytical standard.
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| Toxicity/Toxicokinetics |
Acetamiprid-d3 is intended for research use only and is not approved for human therapeutic applications. Acetamiprid, the parent compound, is a neonicotinoid insecticide with potential toxicity to non-target organisms. Appropriate safety precautions should be taken when handling the compound, including wearing gloves and protective eyewear. The compound should not be inhaled, swallowed, or allowed to contact skin and eyes.
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| References | |
| Additional Infomation |
Acetamiprid-d3 (CAS 1353869-35-8) is a deuterium-labeled form of acetamiprid, a neonicotinoid insecticide. It has a molecular weight of 225.69 g/mol and formula C10H8D3ClN4. The compound is intended for use as an internal standard for the quantification of acetamiprid by GC- or LC-MS. Acetamiprid acts as an agonist of insect nAChRs. The compound is also known as 啶虫脒-D3. It is not approved for clinical use.
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| Molecular Formula |
C10H11CLN4
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|---|---|
| Molecular Weight |
225.6926
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| Exact Mass |
225.086
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| CAS # |
1353869-35-8
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| Related CAS # |
Acetamiprid;135410-20-7
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| PubChem CID |
117064438
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
1.4
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
15
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| Complexity |
280
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])N(CC1=CN=C(C=C1)Cl)C(=NC#N)C
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| InChi Key |
WCXDHFDTOYPNIE-BMSJAHLVSA-N
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| InChi Code |
InChI=1S/C10H11ClN4/c1-8(14-7-12)15(2)6-9-3-4-10(11)13-5-9/h3-5H,6H2,1-2H3/i2D3
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| Chemical Name |
N-[(6-chloropyridin-3-yl)methyl]-N'-cyano-N-(trideuteriomethyl)ethanimidamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4309 mL | 22.1543 mL | 44.3086 mL | |
| 5 mM | 0.8862 mL | 4.4309 mL | 8.8617 mL | |
| 10 mM | 0.4431 mL | 2.2154 mL | 4.4309 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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