| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Abieslactone targets multiple pathways in cancer cells. It induces G1 arrest via upregulation of p53/p21 and downregulation of CDK2/cyclin D1. It also triggers mitochondrial apoptosis through the Bax/Bcl-2/caspase-9/-3 pathway. It modulates the ROS/Akt pathway. Its primary target is not a single protein but rather a network of signaling pathways.
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|---|---|
| ln Vitro |
In vitro, Abieslactone exhibits potent anti-proliferative activity against human tumor cell lines, including SMMC7721 cells, while sparing normal cells (IC50 > 50 µM). Its activity is measured in cell viability assays, where it shows significant inhibition of cancer cell growth.
|
| ln Vivo |
In vivo, Abieslactone has been shown to possess anti-tumor promoting activity. While specific in vivo data are not detailed, its mechanism of inducing apoptosis and cell cycle arrest suggests it could be effective in animal models of cancer.
|
| Enzyme Assay |
The in vitro enzyme/receptor binding assay for Abieslactone is not standard, as it acts through multiple pathways. Its effects on specific targets, such as the induction of apoptosis, are studied in cellular assays. Researchers may measure its ability to activate caspases or modulate the expression of Bcl-2 family proteins using biochemical methods.
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| Cell Assay |
Cellular in vitro experiments for Abieslactone are conducted using various cancer cell lines, such as SMMC7721 cells. Cells are treated with the compound, and cell viability is assessed using MTT or similar assays to determine the IC50. Apoptosis is measured using Annexin V staining or caspase activity assays. Cell cycle analysis is performed using flow cytometry.
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| Animal Protocol |
In vivo animal experiments for Abieslactone would involve using mouse xenograft models bearing human tumor cells. The compound would be administered, and tumor growth would be monitored over time. At the end of the study, tumors would be harvested for analysis of apoptosis and cell cycle markers to confirm the mechanism of action.
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| References | |
| Additional Infomation |
According to reports, Abieslactone is found in fir (Abies pindrow), veitchii, and other organisms for which relevant data exists.
|
| Molecular Formula |
C31H48O3
|
|---|---|
| Molecular Weight |
468.711030006409
|
| Exact Mass |
468.36
|
| CAS # |
33869-93-1
|
| PubChem CID |
161784
|
| Appearance |
Typically exists as solid at room temperature
|
| Source |
Originated from plants: Lepidoziaceae Lepidozia reptans (L.) Dumort.
|
| LogP |
7.504
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
34
|
| Complexity |
914
|
| Defined Atom Stereocenter Count |
9
|
| SMILES |
O(C)[C@@H]1CC[C@@]2(C)[C@@H](CC=C3[C@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@@H]4C=C(C)C(=O)O4)CC[C@]23C)C1(C)C
|
| InChi Key |
RHCAOVDBFCREAC-XOUPGTJXSA-N
|
| InChi Code |
InChI=1S/C31H48O3/c1-19(17-21-18-20(2)27(32)34-21)22-11-15-31(7)24-9-10-25-28(3,4)26(33-8)13-14-29(25,5)23(24)12-16-30(22,31)6/h9,18-19,21-23,25-26H,10-17H2,1-8H3/t19-,21-,22-,23-,25+,26-,29-,30-,31+/m1/s1
|
| Chemical Name |
(2R)-2-[(2R)-2-[(3R,5R,9S,10R,13R,14R,17R)-3-methoxy-4,4,10,13,14-pentamethyl-2,3,5,6,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-17-yl]propyl]-4-methyl-2H-furan-5-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1335 mL | 10.6676 mL | 21.3352 mL | |
| 5 mM | 0.4267 mL | 2.1335 mL | 4.2670 mL | |
| 10 mM | 0.2134 mL | 1.0668 mL | 2.1335 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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