| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The primary target of Palmitic acid-d3 is not the compound itself but the parent compound Palmitic acid, which is a long-chain saturated fatty acid. Palmitic acid can induce the expression of GRP78 and CHOP in mouse granulosa cells. Palmitic acid-d3 is used as an internal standard for the quantification of Palmitic acid in biological samples.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Palmitic acid-d3 itself does not exhibit direct in vitro biological activity because it is a stable isotope-labeled internal standard. However, the parent compound Palmitic acid can induce the expression of GRP78 and CHOP in mouse granulosa cells. Palmitic acid-d3 is used as an internal standard to accurately measure Palmitic acid concentrations in biological samples. |
| ln Vivo |
Palmitic acid-d3 is not intended for in vivo administration as a therapeutic agent; it is used as an analytical standard. The in vivo activity of the parent compound Palmitic acid is associated with its role as a fatty acid and its effects on cellular stress pathways. Palmitic acid-d3 is utilized in pharmacokinetic and metabolic studies to track the distribution, metabolism, and excretion of Palmitic acid.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for Palmitic acid-d3 are not typically performed, as it is an analytical standard. Instead, the compound is used in analytical method development and validation. Quality control assays involve HPLC or LC-MS/MS to verify isotopic purity (≥98 atom % D) and chemical purity (≥98%). The compound is tested for stability under various storage conditions.
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| Cell Assay |
Palmitic acid-d3 itself is not tested in in vitro cellular assays for biological activity, as it is an analytical standard. Its utility is assessed by its performance as an internal standard in analytical workflows, including its ability to provide accurate quantification in LC-MS/MS analyses. The compound is used in quality control applications for Palmitic acid analysis.
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| Animal Protocol |
In vivo animal studies for Palmitic acid-d3 are not conducted as therapeutic efficacy studies. Instead, the compound is administered to animals as a tracer in pharmacokinetic studies to quantify the parent compound Palmitic acid in plasma, tissues, and excreta. These studies help determine the absorption, distribution, metabolism, and excretion (ADME) profile of Palmitic acid.
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| ADME/Pharmacokinetics |
Palmitic acid-d3 has a molecular formula of C16H29D3O2 and a molecular weight of 259.45. It has a purity of ≥98% and an isotopic enrichment of ≥98 atom % D. It appears as a solid. The compound is stable under recommended storage conditions. It is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of Palmitic acid-d3 is not extensively characterized, as it is an analytical standard rather than a therapeutic agent. The parent compound Palmitic acid is a common dietary fatty acid that is generally recognized as safe. Palmitic acid-d3 is intended for research use only and is not approved for clinical use. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
Palmitic acid (d3) is a long-chain fatty acid and a deuterated fatty acid.
Palmitic acid-d3 (CAS 75736-53-7) is the deuterium-labeled form of Palmitic acid. Palmitic acid is a long-chain saturated fatty acid commonly found in both animals and plants. Palmitic acid can induce the expression of GRP78 and CHOP in mouse granulosa cells. Palmitic acid-d3 is used as an internal standard for the quantification of Palmitic acid. It is intended for research use only and is not approved for clinical use. |
| Molecular Formula |
C16H29D3O2
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|---|---|
| Molecular Weight |
259.44
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| Exact Mass |
256.24
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| CAS # |
75736-53-7
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| Related CAS # |
Palmitic acid;57-10-3;Palmitic acid sodium;408-35-5
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| PubChem CID |
12358530
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| Appearance |
White to off-white solid powder
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| Density |
0.862 g/cm3 at 25 °C
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| Boiling Point |
271.5 °C(lit.) at 100 mmHg
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| Melting Point |
61-64 °C
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| LogP |
5.552
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
18
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| Complexity |
178
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])CCCCCCCCCCCCCCC(=O)O
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| InChi Key |
IPCSVZSSVZVIGE-FIBGUPNXSA-N
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| InChi Code |
InChI=1S/C16H32O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16(17)18/h2-15H2,1H3,(H,17,18)/i1D3
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| Chemical Name |
16,16,16-trideuteriohexadecanoic acid
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| Synonyms |
palmitic acid d3
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (385.45 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.64 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8545 mL | 19.2723 mL | 38.5446 mL | |
| 5 mM | 0.7709 mL | 3.8545 mL | 7.7089 mL | |
| 10 mM | 0.3854 mL | 1.9272 mL | 3.8545 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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