| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Human Endogenous Metabolite
|
|---|---|
| ln Vitro |
Octocadecane (12.5-100 μg/ml; 18 hours) exhibits high cytotoxic action against B16F10-Nex2 cells, with an IC50 value of 41.08 μg/ml[1].
|
| ln Vivo |
Octocadecane (500 μg; daily injection at peripheral sites related to blast cell transplantation; 35 days) exhibits strong anticancer effects and considerably slows the growth of tumors [1].
|
| Cell Assay |
Cell Cytotoxicity Assay[1]
Cell Types: B16F10-Nex2 cells Tested Concentrations: 12.5, 25, 50, 100 μg/ml Incubation Duration: 18 h Experimental Results: Displayed strong cytotoxic activity on B16F10-Nex2 cells, with an IC50 value of 41.08 μg/ml |
| Animal Protocol |
Animal/Disease Models: C57Bl/6 mice with B16F10-Nex2 Cellsl[1]
Doses: 500 μg Route of Administration: Injected at peripheral sites in relation to the original cell grafting; daily; 35 days Experimental Results: Resulted in a significant delay of tumor progression with a significant antitumor effect. The survival rate of treated groups was Dramatically increased. |
| ADME/Pharmacokinetics |
Absorption, Distribution and Excretion
Samples were collected from the liver, heart, kidneys, muscle, and adipose tissue (perirhinal and subcutaneous) of six cattle for hydrocarbon composition analysis. Qualitative and quantitative analyses were performed using gas chromatography and gas chromatography-mass spectrometry. Despite varying proportions, a series of homologous n-alkanes with carbon chain lengths ranging from n-C12 to n-C31 were found in all samples. Additionally, isoprene hydrocarbons phytane and phyene (phytane-1 and phyene-2) were identified. (These findings are relevant to human health from consuming hydrocarbon-contaminated meat.) /n-Alkanes/ Arthrobacter nana KCC B35, isolated from blue-green cushions covered with oil deposits along the Arabian Gulf coast, was able to grow well using C10 to C40 n-alkanes as its sole carbon and energy source. Growth on C20 to C40 alkanes was even superior to growth on C10 to C18 alkanes. After culturing biomass samples with either n-octacosane (C28) or n-nonacosane (C29) for 6 hours, these compounds became the major alkanes comprising the cellular hydrocarbon component. Even-chain hexadecane (C16) and odd-chain pentadecane (C15) were the second most abundant alkanes in C28 and C29 cultured cells, respectively. Compared to control cells not incubated with hydrocarbon compounds, cells incubated with n-hexadecane accumulated a higher proportion of C16 fatty acids in their lipids. On the other hand, no fatty acids with the same chain length were detected in cells incubated with C28 and C29, but the fatty acid profiles of the cellular lipids suggested that these ultra-long-chain alkanes may have undergone medium-chain oxidation. This activity makes Tobacco Monotyphimurium KCC B35 suitable for use in formulations for the bioremediation of heavy oil deposit contamination. |
| Toxicity/Toxicokinetics |
Toxicity Summary
Identification and Uses: Octacoane is a higher n-alkane containing 28 carbon atoms (C28). Human Exposure and Toxicity: No data are currently available. Animal Studies: A series of homologues of n-alkane, ranging in carbon chain length from n-C12 to n-C31, were found in all samples from bovine liver, heart, kidney, muscle, and adipose tissue. |
| References |
|
| Additional Infomation |
waxy hydrocarbon, insoluble in water. Octacosandecane is a straight-chain alkane containing 28 carbon atoms. It is a plant metabolite. Octacosandecane has been reported to exist in Vanilla madagascariensis, Andrachne rotundifolia, and other organisms with relevant data. See also: Moringa leaf oil (partial).
|
| Molecular Formula |
C28H58
|
|---|---|
| Molecular Weight |
394.76
|
| Exact Mass |
394.454
|
| CAS # |
630-02-4
|
| Related CAS # |
Octacosane-d58;16416-33-4
|
| PubChem CID |
12408
|
| Appearance |
White to off-white solid powder
|
| Boiling Point |
278 °C15 mm Hg(lit.)
|
| Melting Point |
57-62 °C(lit.)
|
| Flash Point |
227 °C
|
| Vapour Pressure |
<1 mm Hg ( 20 °C)
|
| Index of Refraction |
1.4330 (70ºC)
|
| LogP |
11.168
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
0
|
| Rotatable Bond Count |
25
|
| Heavy Atom Count |
28
|
| Complexity |
218
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
ZYURHZPYMFLWSH-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C28H58/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h3-28H2,1-2H3
|
| Chemical Name |
octacosane
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5332 mL | 12.6659 mL | 25.3318 mL | |
| 5 mM | 0.5066 mL | 2.5332 mL | 5.0664 mL | |
| 10 mM | 0.2533 mL | 1.2666 mL | 2.5332 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
