| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
As a click chemistry reagent, N3-TEMPO does not have a defined biological target. Its role is to serve as a chemical tool for bioorthogonal labeling and spin labeling. The azide group enables bioorthogonal reactions with alkyne-bearing molecules, while the TEMPO moiety provides a stable radical for EPR spectroscopy. The compound is used to investigate protein and nucleic acid dynamics.
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| ln Vitro |
In cell-free biochemical systems, N3-TEMPO is used as a click chemistry reagent for the labeling and modification of biomolecules. The azide group undergoes CuAAc reactions with molecules bearing alkyne groups or SPAAC reactions with DBCO or BCN-containing molecules. The TEMPO moiety serves as a spin label for EPR spectroscopy.
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| ln Vivo |
This compound does not exhibit direct cellular activity as it is a chemical reagent for bioconjugation. Biomolecules labeled with N3-TEMPO can be delivered into cells for various functional studies. The azide functionality allows for subsequent click chemistry labeling within cells, enabling the study of biomolecule localization, trafficking, and dynamics. The TEMPO moiety can be used for EPR spectroscopy to study protein and nucleic acid dynamics.
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| Enzyme Assay |
The standard procedure for using N3-TEMPO involves click chemistry reactions between the azide group and alkyne-bearing molecules. The CuAAc reaction is typically performed in aqueous buffer with copper sulfate, sodium ascorbate, and a ligand such as TBTA. The SPAAC reaction is performed with DBCO or BCN-containing molecules without copper catalysis. The efficiency of labeling is assessed by mass spectrometry, HPLC, or EPR spectroscopy.
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| Cell Assay |
No cell-based experimental protocols are directly applicable to N3-TEMPO as it is a chemical reagent. Biomolecules labeled with N3-TEMPO can be evaluated in cell culture experiments. Typical protocols include treating cells with azide-containing biomolecules followed by click chemistry labeling with fluorescent or affinity tags. The labeled biomolecules can be analyzed by fluorescence microscopy, flow cytometry, or EPR spectroscopy.
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| Animal Protocol |
N3-TEMPO is not administered to animals as it is a chemical reagent for labeling. Biomolecules labeled with this compound may be evaluated in animal models for various applications. Typical studies involve administration of labeled biomolecules to animals via appropriate routes. The azide functionality can be used for in vivo click chemistry labeling if suitable bioorthogonal reaction conditions are available.
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| ADME/Pharmacokinetics |
As a chemical reagent, N3-TEMPO does not have established pharmacokinetic properties. The compound is used in vitro for bioconjugation and labeling. Its stability, solubility, and reactivity are relevant for its use in chemical synthesis. The compound has a melting point of 68-71°C and is typically stored at low temperatures for research use. It is not intended for therapeutic use.
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| Toxicity/Toxicokinetics |
The compound is not intended for therapeutic use and lacks established toxicity profiles. Standard laboratory safety precautions should be observed when handling this chemical reagent. The compound contains an azide group, which can be potentially explosive when heated or subjected to shock. Appropriate safety measures should be taken. The compound is for research use only and is not for human therapeutic applications.
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| References | |
| Additional Infomation |
N3-TEMPO is a research-grade chemical supplied for click chemistry and EPR spectroscopy applications. It is not an approved pharmaceutical and has no clinical trial history. The compound is an azide-functionalized TEMPO spin label used for bioorthogonal labeling. This product is intended for research use only.
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| Molecular Formula |
C9H17N4O*
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|---|---|
| Molecular Weight |
197.26
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| Exact Mass |
198.148
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| CAS # |
63697-61-0
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| PubChem CID |
149016
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| Appearance |
Yellow to orange solid powder
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| LogP |
2.098
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
14
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| Complexity |
250
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| Defined Atom Stereocenter Count |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0695 mL | 25.3473 mL | 50.6945 mL | |
| 5 mM | 1.0139 mL | 5.0695 mL | 10.1389 mL | |
| 10 mM | 0.5069 mL | 2.5347 mL | 5.0695 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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