| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| Targets |
GSK-3β 10.2 μM (IC50) Plasmodium CDK5 1.5 μM (IC50) vacuolar ATPases Malaria HSV-1
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|---|---|
| ln Vitro |
Manzamine A (5-50 µM, 18 h) hydrochloride reduces tau phosphorylation as determined by ELISA[1]. Manzamine A (10 µM) hydrochloride inhibits 30% of the development of yeast S. cerevisiae[2]. In yeast, manzamine A hydrochloride exhibits a few expanded vacuoles[2]. Pancreatic cancer cells and non-malignant Vero cells exhibit increased acidity in response to manzamine A (2.5-10 µM, 24 h) hydrochloride[2]. In SIRC cells, HSV-1 infection is inhibited by manzamine A (1 µM, 24 h) hydrochloride[4]. The antimalarial activity of manzamine A hydrochloride is demonstrated by IC50 values of 8.0 nM (D6 clone) and 11 nM (W2 clone)[5].
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| ln Vivo |
Infected mice treated with manzamine A (50 and 100 mol/kg, po or ip) hydrochloride do not develop the rodent malaria parasite Plasmodium berghei [6]. Swine survival is extended to 20 days with manzamine A (8 mg/kg, ip, daily for 8 consecutive days) hydrochloride[7].
|
| Cell Assay |
Cell Viability Assay[4]
Cell Types: SIRC cell Tested Concentrations: 0.1, 0.5, 1, 2, 3, 5, and 10 µM Incubation Duration: 72 h Experimental Results: Inhibited SIRC cell viability with an IC50 of 5.6 µM. |
| Animal Protocol |
Animal/Disease Models: Plasmodium berghei in infected mice[6]
Doses: 50 or 100 mol/kg Route of Administration: intraperitoneal (ip)injection or oral administration (po) Experimental Results: Inhibited the growth of the rodent malaria parasite Plasmodium berghei. Prolonged the survival of highly parasitaemic mice. |
| References |
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| Molecular Formula |
C36H44N4O
|
|---|---|
| Molecular Weight |
548.761
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| Exact Mass |
584.328
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| CAS # |
104264-80-4
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| Related CAS # |
Manzamine A;104196-68-1
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| PubChem CID |
49797386
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| Appearance |
Light yellow to brown solid powder
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
42
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| Complexity |
1050
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| Defined Atom Stereocenter Count |
5
|
| SMILES |
C123C([H])([H])C4(C(=C(C(C(C(C([H])([H])N4C1(C1(C(=C(c4c5[n]([H])c6c(c(c(c([H])c6c5c([H])c([H])n4)[H])[H])[H])C2([H])C([H])([H])C([H])([H])N(C(C(C(C(C(=C(C(C1([H])[H])([H])[H])[H])[H])([H])[H])([H])[H])([H])[H])([H])[H])C3([H])[H])[H])O[H])[H])([H])[H])([H])[H])([H])[H])[H])[H])[H]
|
| InChi Key |
DIVWQABXFSWTEF-YTGAITGWSA-N
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| InChi Code |
InChI=1S/C36H44N4O.ClH/c41-36-18-10-4-1-2-5-11-20-39-22-17-30(35(25-39)23-26-13-7-3-6-12-21-40(26)34(35)36)29(24-36)32-33-28(16-19-37-32)27-14-8-9-15-31(27)38-33;/h1,4,7-9,13-16,19,24,26,30,34,38,41H,2-3,5-6,10-12,17-18,20-23,25H2;1H/b4-1-,13-7-;/t26-,30-,34+,35-,36-;/m0./s1
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| Chemical Name |
(1R,2R,4R,5Z,12R,13S,16Z)-25-(9H-pyrido[3,4-b]indol-1-yl)-11,22-diazapentacyclo[11.11.2.12,22.02,12.04,11]heptacosa-5,16,25-trien-13-ol;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 5.88 mg/mL (10.05 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8223 mL | 9.1115 mL | 18.2229 mL | |
| 5 mM | 0.3645 mL | 1.8223 mL | 3.6446 mL | |
| 10 mM | 0.1822 mL | 0.9111 mL | 1.8223 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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