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| Other Sizes |
| Targets |
Non-cleavable Linker
As a chemical linker for bioconjugation, Mal-amido-PEG5-C2-NHS ester does not have a defined biological target. Its role is to serve as a non-cleavable connector between biomolecules such as antibodies and drugs or other functional moieties. The maleimide group allows for site-specific conjugation to thiol groups, while the NHS ester enables labeling of primary amines. The non-cleavable nature of the linker provides stable attachment of payloads. |
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| ln Vitro |
In cell-free biochemical systems, this linker is used to label the primary amines of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The maleimide group reacts with thiol groups to form stable thioether bonds. The PEG5 spacer improves the solubility and stability of the resulting conjugates in aqueous environments. The linker is commonly used in ADC synthesis and other bioconjugation research applications.
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| ln Vivo |
This compound does not exhibit direct cellular activity as it is a chemical linker rather than a bioactive molecule. Bioconjugates constructed using this linker can be delivered to target cells where the antibody binds to cell surface antigens, followed by internalization. The non-cleavable nature of the linker means that the payload remains attached to the antibody until antibody degradation. This provides stable payload delivery to target cells.
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| Enzyme Assay |
The cell-free assay for this linker involves conjugation reactions between the maleimide and thiol groups on proteins or peptides, and between the NHS ester and primary amines. The reaction is typically performed in aqueous buffer at pH 6.5-7.5 for maleimide-thiol conjugation and pH 7.4-8.5 for NHS ester-amine conjugation. The efficiency of conjugation is assessed by mass spectrometry, SDS-PAGE, or HPLC analysis.
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| Cell Assay |
No cell-based experimental protocols are directly applicable to this linker as it is a chemical synthesis reagent. Bioconjugates synthesized using this linker can be evaluated in cell culture experiments. Typical protocols include treating cells with the conjugate at various concentrations and assessing cellular uptake, target engagement, and functional effects. The specificity of the conjugate is evaluated using appropriate control experiments.
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| Animal Protocol |
This linker is not administered to animals as it is a chemical intermediate for bioconjugate synthesis. Bioconjugates incorporating this linker may be evaluated in animal models for pharmacokinetics and efficacy. Typical studies involve administration of the conjugate to animals via appropriate routes. The non-cleavable nature of the linker provides stable payload attachment. Pharmacodynamic endpoints are assessed based on the specific application.
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| ADME/Pharmacokinetics |
As a chemical reagent, this compound does not have established pharmacokinetic properties. The pharmacokinetics of bioconjugates incorporating this linker depend on the biomolecule, payload, and overall conjugate properties. The non-cleavable nature of the linker influences payload release kinetics. The PEG5 spacer may affect the circulation time and biodistribution. The compound itself is not intended for therapeutic use.
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| Toxicity/Toxicokinetics |
The compound is not intended for therapeutic use and lacks established toxicity profiles. Standard laboratory safety precautions should be observed when handling this chemical reagent. The compound is typically stored in pure form at -20°C; in solvent at -80°C for 6 months or at -20°C for 1 month. Appearance is a viscous liquid, colorless to light yellow. Purity is ≥98.0%.
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| References |
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| Additional Infomation |
Mal-amido-PEG5-C2-NHS ester is a research-grade chemical supplied for bioconjugation and ADC synthesis applications. It is not an approved pharmaceutical and has no clinical trial history. The compound is a non-cleavable ADC linker containing a maleimide group and an NHS ester. The NHS ester can be used to label the primary amines of proteins, amine-modified oligonucleotides, and other amine-containing molecules. This product is intended for research use only.
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| Molecular Formula |
C24H35N3O12
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|---|---|
| Exact Mass |
557.222
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| CAS # |
1315355-92-0
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| PubChem CID |
118798955
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
-3.1
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
23
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| Heavy Atom Count |
39
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| Complexity |
844
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCOCCOCCNC(=O)CCN2C(=O)C=CC2=O
|
| InChi Key |
CBUSUMKXBREGEQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H35N3O12/c28-19(5-8-26-20(29)1-2-21(26)30)25-7-10-35-12-14-37-16-18-38-17-15-36-13-11-34-9-6-24(33)39-27-22(31)3-4-23(27)32/h1-2H,3-18H2,(H,25,28)
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[2-[3-(2,5-dioxopyrrol-1-yl)propanoylamino]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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