| Size | Price | Stock | Qty |
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| ln Vitro |
MAO-B-IN-22 (compound 6h) (2.5-50 μM, 24 h) can enhance cell viability and avert oxidative damage brought on by hydrogen peroxide [1].
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| ln Vivo |
MAO-B-IN-22 (compound 6h) (53.5 mg/kg, oral gavage, once daily for 3 weeks) can increase dopaminergic neurotransmitter levels and reduce ROS-induced oxidative damage levels by inhibiting MAO-B Attenuating the motor impairment in mice can alleviate the movement disorders in MPTP-treated mice and may improve the symptoms of PD in vivo [1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: PC-12 cells Tested Concentrations: 2.5 μM, 6h) (0.5-10 μM, 24 h) Can be dosed to reduce LPS-induced NO production and has anti-neuromodulatory activity[1]. 10.0 μM, 50.0 μM Incubation Duration: 24 hrs (hours) Experimental Results: Cell viability increased to 59.8%, 69.6% and 77.2% of the control value at doses of 2.5 μM, 10.0 μM and 50.0 μM, respectively. Western Blot Analysis[1] Cell Types: BV-2 Cell Tested Concentrations: 0.5 μM, 2.5 μM, 10.0 μM Incubation Duration: 24 hrs (hours) Experimental Results: At a concentration of 0.5, LPS-induced NO production was Dramatically diminished by 13.5%, 28.0%, and 76.1 % are μM, 2.5 μM and 10.0 μM respectively. Inhibits lipopolysaccharide-induced ROS release, with inhibition rates of 21.2% and 99.0% at concentrations of 2.5 μM and 10.0 μM, respectively. Dramatically inhibits the phosphorylation of IκBα into p-IκBα and inhibits the translocation of p65 from the cytoplasm to the nucleus. |
| Animal Protocol |
Animal/Disease Models: C57BL/6C mice [1]
Doses: 53.5 mg/kg Route of Administration: po (oral gavage), one time/day for 3 weeks Experimental Results: Significant improvement in traction test scores, reduction in BWT time, T turns, and total number of T . Restores DA levels and reduces MDA levels. |
| References |
[1]. Zhongcheng Cao, et al. Discovery of novel 2-hydroxyl-4-benzyloxybenzyl aniline derivatives as potential multifunctional agents for the treatment of Parkinson's disease. Eur J Med Chem. 2023 Mar 5;249:115142.
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| Molecular Formula |
C20H18FNO2
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|---|---|
| Molecular Weight |
323.37
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| Exact Mass |
323.13
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| Elemental Analysis |
C, 74.29; H, 5.61; F, 5.88; N, 4.33; O, 9.90
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| CAS # |
2902600-76-2
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| SMILES |
OC1=C(C=CC(OCC2=CC=C(C=C2)F)=C1)CNC3=CC=CC=C3
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| InChi Key |
PJSROCSXWXZWPV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H18FNO2/c21-17-9-6-15(7-10-17)14-24-19-11-8-16(20(23)12-19)13-22-18-4-2-1-3-5-18/h1-12,22-23H,13-14H2
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| Chemical Name |
5-((4-fluorobenzyl)oxy)-2-((phenylamino)methyl)phenol
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| Synonyms |
compound 6h; MAO B-IN-22; MAO-B IN-22; MAO-B-IN 22
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0924 mL | 15.4622 mL | 30.9243 mL | |
| 5 mM | 0.6185 mL | 3.0924 mL | 6.1849 mL | |
| 10 mM | 0.3092 mL | 1.5462 mL | 3.0924 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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