| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Targets |
Fingolimod-d4 hydrochloride is the deuterated form of Fingolimod, which is an antagonist of S1P, an extracellular lipid mediator. Fingolimod has been used as an immunomodulating drug to treat multiple sclerosis (MS). The deuterated version serves as a tracer for quantitation.
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| ln Vitro |
Drug molecules have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantitation during the drug development process. Due to its potential to alter the pharmacokinetic and metabolic profiles of medications, deuteration has drawn attention[1].
The in vitro activity of Fingolimod-d4 hydrochloride mirrors that of Fingolimod, which is an S1P receptor modulator. The deuterated form is used as an internal standard to accurately quantify Fingolimod in cell-based experiments. |
| ln Vivo |
In vivo, Fingolimod-d4 hydrochloride is used as a tracer to study the pharmacokinetics and metabolism of Fingolimod. Fingolimod is an immunomodulating drug used to treat multiple sclerosis.
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| Enzyme Assay |
The primary application of Fingolimod-d4 hydrochloride is as an internal standard in LC-MS/MS analysis. A known amount of the labeled compound is added to biological samples (e.g., plasma). Following extraction, the sample is analyzed by LC-MS/MS.
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| Cell Assay |
In cellular assays, Fingolimod-d4 hydrochloride is used to quantify the intracellular concentration of Fingolimod. This allows researchers to study drug uptake and accumulation in cell lines.
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| Animal Protocol |
In animal studies, Fingolimod-d4 hydrochloride is utilized as an internal standard for the quantification of Fingolimod in pharmacokinetic studies. Following administration to animal models, blood samples are collected at various time points and analyzed by LC-MS/MS.
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| ADME/Pharmacokinetics |
Fingolimod-d4 hydrochloride has an isotopic enrichment of 99% ²H. It has a molecular weight of 347.96. The compound should be stored at room temperature.
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| Toxicity/Toxicokinetics |
Fingolimod-d4 hydrochloride is intended for research use only and is not for therapeutic or diagnostic use in humans. As a stable isotope, it is non-radioactive and poses minimal radiological risk. Its toxicological profile is expected to be similar to that of Fingolimod, which is generally well-tolerated.
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| References |
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| Additional Infomation |
Fingolimod-d4 hydrochloride is a stable isotope-labeled compound used as an internal standard for the bioanalysis of Fingolimod. It is an essential tool in drug development for studying the pharmacokinetics of this important immunomodulator.
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| Molecular Formula |
C19H33NO2.HCL
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|---|---|
| Molecular Weight |
343.93176
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| Exact Mass |
347.25
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| CAS # |
1346604-90-7
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| Related CAS # |
Fingolimod; 162359-55-9
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| PubChem CID |
71316986
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
23
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| Complexity |
258
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCC1=CC=C(C=C1)CCC(CO)(CO)N.Cl
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| InChi Key |
SWZTYAVBMYWFGS-JWIOGAFXSA-N
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| InChi Code |
InChI=1S/C19H33NO2.ClH/c1-2-3-4-5-6-7-8-17-9-11-18(12-10-17)13-14-19(20,15-21)16-22;/h9-12,21-22H,2-8,13-16,20H2,1H3;1H/i15D2,16D2;
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| Chemical Name |
2-amino-1,1,3,3-tetradeuterio-2-[2-(4-octylphenyl)ethyl]propane-1,3-diol;hydrochloride
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| Synonyms |
FTY720-d4; Fingolimod-d4 hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9076 mL | 14.5378 mL | 29.0757 mL | |
| 5 mM | 0.5815 mL | 2.9076 mL | 5.8151 mL | |
| 10 mM | 0.2908 mL | 1.4538 mL | 2.9076 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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