| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Ki: 40.05 µM (TEM-1 beta-lactamase)[1]
TEM-1 beta-lactamase, a bacterial enzyme responsible for resistance to beta-lactam antibiotics (e.g., penicillins). It also acts as a glycosidic surfactant, interacting with lipid bilayers and membrane proteins. |
|---|---|
| ln Vitro |
In vitro, Cymal-6 is a potent inhibitor of TEM-1 beta-lactamase, with a Ki value of 40.05 uM. It also functions as a non-ionic detergent and can be used as a glycosidic surfactant for the solubilization and stabilization of membrane proteins in their native state. This dual functionality makes it a valuable biochemical research tool.
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| ln Vivo |
Specific in vivo activity data for Cymal-6 is not provided. Its primary utility is as an in vitro reagent for biochemical assays, not as a systemically administered agent. Its surfactant properties are applied in laboratory settings, not in living animals for a therapeutic effect.
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| Enzyme Assay |
A non-cellular assay to determine the beta-lactamase inhibition would be a standard enzyme kinetics assay. TEM-1 beta-lactamase is incubated with a chromogenic substrate, such as nitrocefin, which changes color from yellow to red upon hydrolysis, in a buffer. Varying concentrations of Cymal-6 are added to the reaction. The change in absorbance at 486 nm (for nitrocefin) is monitored over time using a spectrophotometer. The Ki value is calculated by analyzing the inhibition patterns in a Lineweaver-Burk plot.
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| Cell Assay |
Cymal-6 is not typically used in cell-based assays to assess bioactivity. It is used in cell biology as a mild detergent. For example, to prepare cell lysates for protein analysis, a protocol would involve harvesting cells, washing them with PBS, and then resuspending them in a lysis buffer containing Cymal-6 (e.g., 0.5-1% w/v) along with protease inhibitors. The mixture is incubated on ice to solubilize the cell membrane and release intracellular proteins.
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| Animal Protocol |
Specific in vivo animal experimental protocols for Cymal-6 are not applicable. As a surfactant, it is not administered to animals as a drug. It is possible to use it in ex vivo applications, but not in standard in vivo efficacy models.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) data is not applicable for this biochemical reagent. The compound is not intended for systemic administration. Its physical-chemical properties, such as its Critical Micelle Concentration (CMC), are more relevant for its use as a detergent.
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| Toxicity/Toxicokinetics |
Cymal-6 is generally considered a laboratory chemical and is handled with standard safety precautions. Avoid contact with skin and eyes, and avoid inhalation of dust. Toxicity information for this specific compound is not detailed in the provided text.
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| References |
[1]. Avci FG, et al. Targeting a hidden site on class A beta-lactamases. J Mol Graph Model. 2018 Sep;84:125-133.
[2]. Ju M, et al. Enantioseparations by capillary electrophoresis using chiral glycosidic surfactants. II. Comparisonof chiral cyclohexyl-alkyl-β-D-maltoside surfactant. Journal of Liquid Chromatography & Related Technologies. 2000, 23(1):35-45. |
| Additional Infomation |
The Ki of 40.05 uM indicates relatively weak inhibition, suggesting that its primary utility is as a surfactant. The cyclohexyl-hexyl chain provides a specific hydrophobic character. It belongs to a class of alkyl maltosides often used in structural biology for the crystallization of membrane proteins, as they are generally milder than ionic detergents like SDS.
|
| Molecular Formula |
C24H44O11
|
|---|---|
| Molecular Weight |
508.60
|
| Exact Mass |
508.288
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| CAS # |
228579-27-9
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| PubChem CID |
447688
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| Appearance |
White to off-white solid powder
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| LogP |
0.9
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
11
|
| Rotatable Bond Count |
12
|
| Heavy Atom Count |
35
|
| Complexity |
590
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| Defined Atom Stereocenter Count |
10
|
| SMILES |
C(CCCO[C@H]1[C@@H]([C@H]([C@@H]([C@@H](CO)O1)O[C@@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)O)O)CCC3CCCCC3
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| InChi Key |
WUCWJXGMSXTDAV-QKMCSOCLSA-N
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| InChi Code |
InChI=1S/C24H44O11/c25-12-15-17(27)18(28)20(30)24(33-15)35-22-16(13-26)34-23(21(31)19(22)29)32-11-7-2-1-4-8-14-9-5-3-6-10-14/h14-31H,1-13H2/t15-,16-,17-,18+,19-,20-,21-,22-,23-,24-/m1/s1
|
| Chemical Name |
(2R,3R,4S,5S,6R)-2-[(2R,3S,4R,5R,6R)-6-(6-cyclohexylhexoxy)-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 250 mg/mL (491.55 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9662 mL | 9.8309 mL | 19.6618 mL | |
| 5 mM | 0.3932 mL | 1.9662 mL | 3.9324 mL | |
| 10 mM | 0.1966 mL | 0.9831 mL | 1.9662 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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