| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
The primary biological targets of cyanidin 3-sambubioside chloride are related to its antioxidant properties. Anthocyanins scavenge free radicals, chelate metal ions, and modulate signaling pathways such as NF-κB and Nrf2. They may also interact with enzymes involved in inflammation and metabolism. The compound's effects are mediated through both direct antioxidant activity and modulation of cellular signaling pathways. Specific molecular targets require further elucidation.
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| ln Vitro |
In vitro studies have demonstrated the antioxidant activity of cyanidin 3-sambubioside chloride and other anthocyanins. The compound scavenges DPPH, ABTS, and other free radicals. It may also exhibit anti-inflammatory activity through inhibition of pro-inflammatory cytokine production and suppression of inflammatory mediators. Anthocyanins have been shown to inhibit enzymes such as α-glucosidase and tyrosinase. Specific IC₅₀ values for radical scavenging and enzyme inhibition have been reported.
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| ln Vivo |
In vivo studies have shown the health benefits of anthocyanins in animal models. Cyanidin glycosides have demonstrated antioxidant, anti-inflammatory, and cardioprotective effects. They may improve vascular function, reduce blood pressure, and have beneficial effects on metabolism. Cyanidin 3-sambubioside is a dietary anthocyanin found in various berries and fruits. Specific in vivo data for this compound have been reported in the nutritional and pharmacological literature.
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| Enzyme Assay |
For antioxidant assays, DPPH radical scavenging, ABTS, and FRAP assays are used. The compound is incubated with the radical-generating system, and absorbance is measured. IC₅₀ values are calculated. For anti-inflammatory assays, macrophages or other cells are stimulated with LPS, and cytokine production is measured by ELISA. For enzyme inhibition assays, the compound is incubated with the enzyme and substrate, and product formation is monitored spectrophotometrically.
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| Cell Assay |
For in vitro cell-based studies, various cell lines such as endothelial cells, macrophages, or cancer cells may be used. Cells are cultured in appropriate media and treated with serial dilutions of cyanidin 3-sambubioside chloride. Cell viability is assessed using MTT or other assays. Markers of oxidative stress, inflammation, or cell signaling are measured by Western blotting, ELISA, or qPCR. Standard protocols for these assays are described in the literature.
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| Animal Protocol |
In vivo animal studies for cyanidin 3-sambubioside chloride typically use mouse or rat models. For antioxidant or anti-inflammatory studies, animals are treated with the compound via oral administration, and markers of oxidative stress or inflammation are measured. For cardioprotection studies, models of cardiovascular disease may be used. The compound may also be studied for its effects on metabolism and obesity. Standard protocols can be adapted from the literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of cyanidin 3-sambubioside chloride have been characterized. Anthocyanins are generally poorly absorbed, with most of the ingested compound reaching the colon where it is metabolized by the gut microbiota. The compound is rapidly eliminated from the circulation. The half-life of anthocyanins in plasma is short (1-2 hours). Specific PK parameters have been reported for various anthocyanins. The chloride salt form is water-soluble.
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| Toxicity/Toxicokinetics |
Toxicological data for cyanidin 3-sambubioside chloride are limited. As a dietary anthocyanin found in many fruits and vegetables, it is considered safe. No significant toxicity has been reported at dietary intake levels. High doses may have mild gastrointestinal effects. Comprehensive toxicological studies are available for anthocyanin-rich extracts rather than for the pure compound.
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| References |
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| Additional Infomation |
See also: Hibiscus sabdariffa Flower (partial).
Cyanidin 3-sambubioside chloride is a research compound used in studies of anthocyanins, plant pigments, and their biological activities. It is found in various berries and fruits. The compound is used as a reference standard for analytical method development (e.g., HPLC, LC-MS) for anthocyanin analysis in foods and biological samples. The compound has been studied for its antioxidant, anti-inflammatory, and potential health-promoting effects. No clinical trials or therapeutic applications have been reported. |
| Molecular Formula |
C26H29CLO15
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|---|---|
| Molecular Weight |
616.95
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| Exact Mass |
616.119
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| CAS # |
33012-73-6
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| PubChem CID |
3084569
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| Appearance |
Brown to black solid powder
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
42
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| Complexity |
852
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| Defined Atom Stereocenter Count |
9
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| SMILES |
C1[C@H]([C@@H]([C@H]([C@@H](O1)O[C@@H]2[C@H]([C@@H]([C@H](O[C@H]2OC3=CC4=C(C=C(C=C4[O+]=C3C5=CC(=C(C=C5)O)O)O)O)CO)O)O)O)O)O.[Cl-]
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| InChi Key |
BWHXEGJOYVNGQH-XOBUMVIESA-N
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| InChi Code |
InChI=1S/C26H28O15.ClH/c27-7-18-20(34)21(35)24(41-25-22(36)19(33)15(32)8-37-25)26(40-18)39-17-6-11-13(30)4-10(28)5-16(11)38-23(17)9-1-2-12(29)14(31)3-9;/h1-6,15,18-22,24-27,32-36H,7-8H2,(H3-,28,29,30,31);1H/t15-,18-,19+,20-,21+,22-,24-,25+,26-;/m1./s1
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| Chemical Name |
(2S,3R,4S,5R)-2-[(2S,3R,4S,5S,6R)-2-[2-(3,4-dihydroxyphenyl)-5,7-dihydroxychromenylium-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]oxyoxane-3,4,5-triol;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6209 mL | 8.1044 mL | 16.2088 mL | |
| 5 mM | 0.3242 mL | 1.6209 mL | 3.2418 mL | |
| 10 mM | 0.1621 mL | 0.8104 mL | 1.6209 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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