| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Colominic acid sodium salt is classified as an antibiotic with anti-infection activity. Its primary application is as a substrate for sialyltransferase enzymes in protein polysialylation research. Polysialic acid (colominic acid) is a form of N-glycosylation that plays important roles in neural development and plasticity. The compound also exhibits antibacterial activity and is used in studying infections caused by Streptococcus pneumoniae and Haemophilus influenzae.
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| ln Vitro |
The N-glycosylation form colominic acid, also known as polysialic acid, is highly selective for a small number of targets in the central nervous system, such as the receptor neuropilin-2 and the cell adhesion molecules neural cell adhesion molecule (NCAM) and synaptic cell adhesion molecule 1 (SynCAM1)[2].
In vitro, colominic acid sodium salt is used as a substrate for CMP-NeuAc:(NeuAcα2→8)n (colominic acid) sialyltransferase. The compound exhibits antibacterial activity. It has an absorption maximum at 530 nm, which can be used for detection and quantification. The compound is used to research protein polysialylation processes. It can be naturally isolated from the cell wall of E. coli or animals. |
| ln Vivo |
No detailed in vivo activity data has been published for colominic acid sodium salt as a therapeutic agent. The compound is primarily used as a research tool for studying protein polysialylation and bacterial infections. It has antibacterial activity that may be relevant for in vivo applications. The compound is used in pharmaceutical formulations including antibiotics and vaccines. Further studies would be needed to evaluate its in vivo efficacy.
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| Enzyme Assay |
The sialyltransferase activity of colominic acid sodium salt can be assessed using in vitro enzyme assays. In a typical assay, CMP-NeuAc (the donor substrate) and colominic acid sodium salt (the acceptor substrate) are incubated with the sialyltransferase enzyme. The transfer of sialic acid residues to the colominic acid polymer is measured using radioactive or fluorescent detection methods. The compound can also be detected by its characteristic red color with an absorption maximum at 530 nm.
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| Cell Assay |
The cellular activity of colominic acid sodium salt is assessed in cell culture models studying protein polysialylation. Cells are treated with the compound, and polysialylation of target proteins is assessed by Western blotting using antibodies specific for polysialic acid (such as the 12E8 antibody). The compound's antibacterial activity is assessed using standard susceptibility testing methods against bacterial strains such as Streptococcus pneumoniae and Haemophilus influenzae. The minimum inhibitory concentration (MIC) can be determined using broth microdilution assays.
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| Animal Protocol |
No detailed in vivo animal model data has been published for colominic acid sodium salt. The compound is a research tool used for studying protein polysialylation and bacterial infections. If in vivo studies were conducted, they would likely involve mouse models of bacterial infection to evaluate the compound's antibacterial efficacy, or mouse models of neural development to study the role of polysialic acid.
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| ADME/Pharmacokinetics |
No detailed pharmacokinetic data has been published for colominic acid sodium salt. The compound is a polysaccharide with a high molecular weight and is typically stored at 4°C, sealed and away from moisture. In solvent, it can be stored at -80°C for 6 months or at -20°C for 1 month. The compound is soluble in aqueous solutions. Further studies would be needed to characterize its absorption, distribution, metabolism, and excretion properties.
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| Toxicity/Toxicokinetics |
No detailed toxicity data has been published for colominic acid sodium salt. As a naturally occurring polysaccharide found in the cell wall of E. coli and animals, it is likely to have a favorable safety profile. The compound is intended for research use only and is not for human therapeutic use. Comprehensive toxicology studies would be required to evaluate its safety profile for potential therapeutic development.
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| References | |
| Additional Infomation |
Colominic acid sodium salt (Polysialic acid sodium salt, CAS# 70431-34-4) is a naturally occurring polysaccharide isolated from E. coli and animals. It has an absorption maximum at 530 nm and gives a red color. The compound is a substrate for CMP-NeuAc:(NeuAcα2→8)n sialyltransferase in protein polysialylation research. It exhibits antibacterial activity and is used in studying bacterial infections. The compound is available with ≥98% purity. It is a form of N-glycosylation.
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| Molecular Formula |
C11H20NNA3O7
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|---|---|
| Molecular Weight |
347.25
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| Exact Mass |
955.227
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| CAS # |
70431-34-4
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| PubChem CID |
57369830
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
22
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| Complexity |
288
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| Defined Atom Stereocenter Count |
4
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| SMILES |
CC(=O)N[C@@H]1[C@H](CC(O[C@@H]1[C@@H](CO)O)O[CH]CO)O.[Na+].[Na+].[Na+]
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| InChi Key |
IPYNPWIUCZLARC-XAIQJKFESA-N
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| InChi Code |
InChI=1S/C11H20NO7.3Na/c1-6(15)12-10-7(16)4-9(18-3-2-13)19-11(10)8(17)5-14;;;/h3,7-11,13-14,16-17H,2,4-5H2,1H3,(H,12,15);;;/q;3*+1/t7-,8+,9?,10+,11+;;;/m0.../s1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 100 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8798 mL | 14.3988 mL | 28.7977 mL | |
| 5 mM | 0.5760 mL | 2.8798 mL | 5.7595 mL | |
| 10 mM | 0.2880 mL | 1.4399 mL | 2.8798 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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