| Size | Price | |
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| Other Sizes |
| Targets |
Azidoacetic acid does not have a specific biological target as it is a click chemistry reagent. Its role is to serve as a chemical tool for bioconjugation and labeling. The azide group enables click chemistry reactions with alkyne-containing molecules, facilitating the attachment of fluorophores, biotin, or other functional groups to biomolecules.
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|---|---|
| ln Vitro |
Azidoacetic acid demonstrates in vitro activity as a click chemistry reagent. The azide group can undergo CuAAc with alkyne-containing molecules, enabling efficient and specific bioconjugation reactions. Its utility is in the synthesis of azide-containing compounds and in the labeling of biomolecules.
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| ln Vivo |
In vivo activity is not applicable to azidoacetic acid as it is a chemical reagent for research use only, not a therapeutic agent. The compound is not intended for administration to living organisms.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not performed for azidoacetic acid, as it is a click chemistry reagent. Its characterization involves assessing its reactivity in click chemistry reactions using HPLC, mass spectrometry, or fluorescence-based methods to confirm conjugation efficiency.
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| Cell Assay |
In vitro cellular assays are not applicable to azidoacetic acid. The compound is used in chemical synthesis and bioconjugation reactions. It can be used to label biomolecules that are subsequently used in cellular assays, but the compound itself is not tested directly on cells.
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| Animal Protocol |
In vivo animal experiments are not applicable to azidoacetic acid. The compound is a research chemical used in bioconjugation and labeling applications and is not intended for in vivo administration.
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| ADME/Pharmacokinetics |
Pharmacokinetic data are not applicable to azidoacetic acid as it is a research reagent, not a therapeutic agent. The compound is not intended for systemic administration.
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| Toxicity/Toxicokinetics |
Azidoacetic acid is not intended for human therapeutic use, and toxicological data are not applicable as it is a research chemical. Standard laboratory safety precautions should be followed when handling this compound, as azides can be potentially explosive.
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| References | |
| Additional Infomation |
Azidoacetic Acid (2-Azidoacetic acid) (CAS#: 18523-48-3) has the molecular formula C2H3N3O2 and a molecular weight of 101.06. It is a click chemistry reagent containing an azide functional group. The compound is used for bioconjugation and labeling applications via copper-catalyzed azide-alkyne cycloaddition (CuAAc). It is for research use only.
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| Molecular Formula |
C2H3N3O2
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|---|---|
| Molecular Weight |
101.06
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| Exact Mass |
101.023
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| CAS # |
18523-48-3
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| PubChem CID |
155605
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| Appearance |
Colorless to light yellow liquid
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| Boiling Point |
98-100ºC 0,1mm
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| Melting Point |
13-15ºC
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| Index of Refraction |
1.47
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| LogP |
0.9
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
7
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| Complexity |
114
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O([H])C(C([H])([H])N=[N+]=[N-])=O
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| InChi Key |
PPXUUPXQWDQNGO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C2H3N3O2/c3-5-4-1-2(6)7/h1H2,(H,6,7)
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| Chemical Name |
2-azidoacetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 250 mg/mL (2473.78 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.8951 mL | 49.4756 mL | 98.9511 mL | |
| 5 mM | 1.9790 mL | 9.8951 mL | 19.7902 mL | |
| 10 mM | 0.9895 mL | 4.9476 mL | 9.8951 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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