| Size | Price | |
|---|---|---|
| 100mg | ||
| Other Sizes |
| Targets |
Myristoylated proteins and lipid-mediated signaling pathways. Azido Myristic Acid is a fatty acid analog that incorporates into proteins undergoing N-terminal myristoylation, allowing for their subsequent detection via click chemistry.
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|---|---|
| ln Vitro |
One possible substrate for enzymes might be azido myristic acid. Because the azide group is so tiny and doesn't exist in any natural molecules, its presence does alter the biodistribution of azido palmitic acid relative to natural palmitic acid, which makes it an excellent substrate for enzymes.
Azido Myristic Acid is used as a chemical probe for labeling myristoylated proteins. It does not have intrinsic biological activity as a drug but is a valuable research tool for studying protein lipidation. It is widely used in proteomics and cell biology. |
| ln Vivo |
No direct in vivo activity as a therapeutic agent; used as a tool for studying protein myristoylation in vivo. It can be administered to cells or organisms to label myristoylated proteins for subsequent detection and identification.
|
| Enzyme Assay |
Click chemistry labeling: Azido Myristic Acid is incorporated into myristoylated proteins in cells or cell lysates. The azide group is then reacted with an alkyne-functionalized detection tag (e.g., biotin-alkyne or fluorescent alkyne) via copper-catalyzed azide-alkyne cycloaddition (CuAAC). Labeled proteins are detected by Western blot, fluorescence imaging, or mass spectrometry.
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| Cell Assay |
Cells are incubated with Azido Myristic Acid to allow incorporation into myristoylated proteins. Cells are then lysed, and the azide-labeled proteins are reacted with alkyne-functionalized tags via click chemistry. Protein labeling is assessed by SDS-PAGE followed by fluorescence scanning or streptavidin blotting. Alternatively, labeled proteins can be enriched for proteomic analysis.
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| Animal Protocol |
Animal models could be used to study protein myristoylation in vivo using Azido Myristic Acid. The compound would be administered to animals, and tissues would be collected for click chemistry-based labeling and detection of myristoylated proteins.
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| ADME/Pharmacokinetics |
Azido Myristic Acid has a molecular weight of 241.33 and a molecular formula of C12H23N3O2. It is a click chemistry reagent with an azide group. Purity: ≥95%. Appearance: white crystalline. Storage: -10degC, desiccated.
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| Toxicity/Toxicokinetics |
No toxicity data has been reported for Azido Myristic Acid. As a research reagent, it is not intended for human therapeutic use. Standard laboratory safety precautions should be followed.
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| References | |
| Additional Infomation |
Azido Myristic Acid is a click chemistry reagent bearing an azide (N3) moiety. It is used for the identification and characterization of myristoylated proteins. It is widely used in proteomics and cell biology. The compound is for research use only.
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| Molecular Formula |
C12H23N3O2
|
|---|---|
| Molecular Weight |
241.33
|
| Exact Mass |
241.179
|
| CAS # |
80667-36-3
|
| PubChem CID |
381108
|
| Appearance |
White to off-white solid-liquid Mixture
|
| LogP |
3.93
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
12
|
| Heavy Atom Count |
17
|
| Complexity |
238
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(CCCCCC(=O)O)CCCCCN=[N+]=[N-]
|
| InChi Key |
HYSGAVCDGKGYSZ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C12H23N3O2/c13-15-14-11-9-7-5-3-1-2-4-6-8-10-12(16)17/h1-11H2,(H,16,17)
|
| Chemical Name |
12-azidododecanoic acid
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1437 mL | 20.7185 mL | 41.4370 mL | |
| 5 mM | 0.8287 mL | 4.1437 mL | 8.2874 mL | |
| 10 mM | 0.4144 mL | 2.0719 mL | 4.1437 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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