| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| Other Sizes |
| Targets |
9-Anthryldiazomethane targets carboxyl groups (-COOH) present in fatty acids, arachidonic acid metabolites, prostaglandins, leukotrienes, thromboxanes, and other carboxylic acid-containing molecules. It forms stable anthrylmethyl esters via a derivatization reaction.
|
|---|---|
| ln Vitro |
The compound selectively reacts with carboxylic acid groups at room temperature without requiring heat or catalysts. The resulting 9-anthrylmethyl ester derivatives are strongly fluorescent and can be detected via HPLC with fluorescence detection. Excitation/emission maxima: 365/412 nm (or 255/412 nm).
|
| ln Vivo |
No in vivo activity data are documented as 9-Anthryldiazomethane is a derivatization reagent for analytical chemistry and fatty acid analysis, not a therapeutic agent for in vivo administration.
|
| Enzyme Assay |
This is a chemical derivatization reaction, not a biological enzyme assay. Free fatty acids or other carboxylic acids are dissolved in organic solvent (e.g., ethyl acetate or acetonitrile). 9-Anthryldiazomethane (ADAM) solution is added (10-100 uM final) and the reaction proceeds at room temperature for 30-60 min. Excess reagent is removed. The resulting fluorescent anthrylmethyl esters are analyzed by reversed-phase HPLC with fluorescence detection (Ex/Em 365/412 nm).
|
| Cell Assay |
Live-cell assays are not performed with 9-Anthryldiazomethane due to its reactivity with carboxylic acids. For cellular fatty acid analysis, lipids are extracted from cell pellets using organic solvents (Folch method, chloroform:methanol). The extracted fatty acids are then derivatized with 9-Anthryldiazomethane as described above, and the fluorescent esters are analyzed by HPLC-FLD.
|
| Animal Protocol |
9-Anthryldiazomethane is not for in vivo animal administration. For tissue fatty acid analysis, tissues are homogenized and lipids are extracted using Folch solution. The extracted free and total fatty acids are derivatized with 9-anthryldiazomethane at room temperature. Fatty acid composition is then analyzed by HPLC with fluorescence detection.
|
| ADME/Pharmacokinetics |
9-Anthryldiazomethane is stable as a solid when stored at -20degC and protected from light. Working solutions should be prepared fresh in organic solvents such as DMF (30 mg/mL) or DMSO (30 mg/mL) immediately before use. The reagent is light-sensitive and may decompose upon prolonged exposure to light. Ex/Em maxima: 365/412 nm in 80:20 acetonitrile:water.
|
| Toxicity/Toxicokinetics |
Toxicity information is limited as this is a research-use derivatization reagent. 9-Anthryldiazomethane is a diazomethane derivative and should be handled with caution. Avoid inhalation, ingestion, and skin contact. Use personal protective equipment, work in a fume hood, and protect from light.
|
| References | |
| Additional Infomation |
This compound is a research-use-only fluorescent labeling reagent, not a drug. It is widely used for HPLC determination of long-chain, short-chain, keto acids, and hydroxy acids in biological samples such as serum, milk, dairy products, and myocardial tissue. Molecular formula: C15H10N2; molecular weight: 218.25. Purity: ≥85%.
|
| Molecular Formula |
C15H10N2
|
|---|---|
| Molecular Weight |
218.2533
|
| Exact Mass |
218.084
|
| CAS # |
10401-59-9
|
| PubChem CID |
3035201
|
| Appearance |
Brown to red solid powder
|
| Melting Point |
65-67°C (lit.)
|
| LogP |
3.722
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
17
|
| Complexity |
297
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
XXDVOJKRZBNPFN-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C15H10N2/c16-17-10-15-13-7-3-1-5-11(13)9-12-6-2-4-8-14(12)15/h1-10H
|
| Chemical Name |
9-(diazomethyl)anthracene
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5819 mL | 22.9095 mL | 45.8190 mL | |
| 5 mM | 0.9164 mL | 4.5819 mL | 9.1638 mL | |
| 10 mM | 0.4582 mL | 2.2910 mL | 4.5819 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
