| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
The primary targets of 9-Hydroxycalabaxanthone include phosphodiesterase 4D2 (PDE4D2) and various cancer cell lines. It inhibits phosphodiesterase 4D2 (PDE4D2) in a cell-free assay with an IC50 of 420 nM. The compound is cytotoxic to HT-29 colon cancer cells with an EC50 of 9.1 µM and is active against P. gingivalis, S. mutans, S. aureus, and S. epidermidis.
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| ln Vitro |
9-hydroxycalabaxanthone suppresses the activities of K1 P. falciparum clones and 3D7 clones with IC50 values of 1.5 μM and 1.2 μM, respectively[1].
In vitro, 9-Hydroxycalabaxanthone demonstrates potent PDE4D2 inhibition with an IC50 of 420 nM in a cell-free assay. It is cytotoxic to HT-29 colon cancer cells with an EC50 of 9.1 µM. The compound also shows antimicrobial activity against various bacterial strains including P. gingivalis, S. mutans, S. aureus, and S. epidermidis. These activities are concentration-dependent and have been characterized in biochemical and cellular assays. |
| ln Vivo |
In vivo activity of 9-Hydroxycalabaxanthone has not been extensively characterized. As a natural xanthone with antioxidant, anti-inflammatory, and anticancer activities, it has potential therapeutic applications. However, detailed in vivo pharmacokinetic and pharmacodynamic studies are limited. The compound is primarily studied for its in vitro biological activities.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for 9-Hydroxycalabaxanthone typically involve PDE4D2 inhibition studies. The enzyme is incubated with varying concentrations of the compound (0.1-1000 nM) in appropriate buffer at 37°C. PDE4D2 activity is measured using fluorogenic or radioisotopic substrates, and the IC50 value is calculated from the dose-response curve. The compound demonstrates concentration-dependent inhibition of PDE4D2 activity with an IC50 of 420 nM.
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| Cell Assay |
In vitro cellular assays for 9-Hydroxycalabaxanthone involve testing its cytotoxic and antimicrobial activities. Cancer cell lines (e.g., HT-29 colon cancer cells) are treated with serial dilutions of the compound (0.1-100 µM) for 48-72 hours. Cell viability is assessed using MTT or CCK-8 assays, and the EC50 value is calculated. For antimicrobial studies, bacterial strains are treated with the compound, and minimum inhibitory concentrations (MIC) are determined.
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| Animal Protocol |
In vivo animal studies for 9-Hydroxycalabaxanthone are limited, as the compound is primarily studied in vitro. For anticancer or anti-inflammatory efficacy testing, animal models of disease could be used. Animals would be treated with the compound via oral or intraperitoneal administration at various doses, and relevant biomarkers would be assessed. However, detailed in vivo protocols are not well established for this compound.
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| ADME/Pharmacokinetics |
9-Hydroxycalabaxanthone has a molecular formula of C24H24O6 and a molecular weight of 408.44. It appears as a yellow powder with a purity of ≥98%. The compound is soluble in DMSO. It is a natural xanthone isolated from Garcinia mangostana. It is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 9-Hydroxycalabaxanthone has not been extensively characterized. As a natural product, it is generally considered to have moderate toxicity. Standard toxicity studies would include assessment of acute oral toxicity, dermal irritation, and repeated-dose toxicity in animal models. The compound is intended for research use only and is not approved for clinical use.
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| References |
[1]. Gamal A Mohamed, et al. Mangostanaxanthones I and II, New Xanthones From the Pericarp of Garcinia Mangostana. Fitoterapia. 2014 Oct;98:215-21.
[2]. Wanna Chaijaroenkul, et al. The in Vitro Antimalarial Interaction of 9-hydroxycalabaxanthone and α-mangostin With mefloquine/artesunate. Acta Parasitol. 2014 Mar;60(1):105-11. |
| Additional Infomation |
9-Hydroxycalazone is a type of flavonoid. It has been reported to exist in plants of the genera Garcinia cowa, Garcinia mangostana, and other organisms with relevant data.
9-Hydroxycalabaxanthone (CAS 35349-68-9) is a xanthone found in Garcinia mangostana with diverse biological activities. It has a molecular formula of C24H24O6 and a molecular weight of 408.44. The compound inhibits PDE4D2 with an IC50 of 420 nM and is cytotoxic to HT-29 colon cancer cells with an EC50 of 9.1 µM. It also shows antimicrobial activity against various bacterial strains. It is intended for research use only and is not approved for clinical use. |
| Molecular Formula |
C24H24O6
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|---|---|
| Molecular Weight |
408.44376
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| Exact Mass |
408.157
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| CAS # |
35349-68-9
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| PubChem CID |
5495929
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
617.6±55.0 °C at 760 mmHg
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| Melting Point |
156 - 156 °C
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| Flash Point |
213.9±25.0 °C
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| Vapour Pressure |
0.0±1.9 mmHg at 25°C
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| Index of Refraction |
1.618
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| LogP |
6.17
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
30
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| Complexity |
723
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=CCC1=C(C(=CC2=C1C(=O)C3=C(C=C4C(=C3O)C=CC(C)(C)O4)O2)O)OC)C
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| InChi Key |
HQWHKELJHUFLGD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H24O6/c1-12(2)6-7-14-19-17(10-15(25)23(14)28-5)29-18-11-16-13(8-9-24(3,4)30-16)21(26)20(18)22(19)27/h6,8-11,25-26H,7H2,1-5H3
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| Chemical Name |
5,9-dihydroxy-8-methoxy-2,2-dimethyl-7-(3-methylbut-2-enyl)pyrano[3,2-b]xanthen-6-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4483 mL | 12.2417 mL | 24.4834 mL | |
| 5 mM | 0.4897 mL | 2.4483 mL | 4.8967 mL | |
| 10 mM | 0.2448 mL | 1.2242 mL | 2.4483 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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