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| Targets |
9-(2-Carboxy-2-cyanovinyl)julolidine does not have a specific biological target. As a fluorescent molecular rotor, its "target" is its local environment, specifically the microviscosity. Its fluorescence quantum yield increases as the free rotation of its molecule is restricted in a more viscous or confined environment, such as when bound to a protein or within a lipid membrane.
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| ln Vitro |
9-(2-Carboxy-2-cyanovinyl)julolidine's "activity" is its fluorescence property. In solution, its fluorescence intensity is low, but it increases significantly in viscous environments or when bound to proteins. This property makes it a sensitive probe for studying protein binding, membrane fluidity, and other processes that affect local microviscosity.
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| ln Vivo |
9-(2-Carboxy-2-cyanovinyl)julolidine does not have in vivo pharmacological activity. It is a chemical probe used for research applications, primarily in vitro, to study biophysical properties like viscosity. It is not administered to animals for therapeutic purposes.
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| Enzyme Assay |
Non-cellular assays for 9-(2-Carboxy-2-cyanovinyl)julolidine involve measuring its fluorescence in solutions of different viscosities to generate a calibration curve. The probe is dissolved in solvents of known viscosity (e.g., glycerol-water mixtures), and its fluorescence intensity or lifetime is measured. This allows for the correlation of fluorescence signal to viscosity, which can then be applied to measure the viscosity of unknown samples.
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| Cell Assay |
In vitro cellular assays for 9-(2-Carboxy-2-cyanovinyl)julolidine involve using it as a fluorescent probe to study cellular viscosity or protein binding. Cells are incubated with the probe, which enters the cells. The fluorescence intensity of the probe inside the cells is then measured using fluorescence microscopy or flow cytometry. Changes in fluorescence can indicate changes in cellular viscosity or the binding of the probe to intracellular proteins.
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| Animal Protocol |
9-(2-Carboxy-2-cyanovinyl)julolidine is not used in in vivo animal studies as a therapeutic agent. Its use is limited to in vitro and ex vivo research applications as a fluorescent probe for studying microviscosity and protein interactions.
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| ADME/Pharmacokinetics |
9-(2-Carboxy-2-cyanovinyl)julolidine is not a drug and does not have pharmacokinetic properties. Its physical and chemical properties are more relevant: it is a hydrophilic fluorescent molecular rotor, with a molecular weight of 268.31 and a molecular formula of C₁₆H₁₆N₂O₂.
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| Toxicity/Toxicokinetics |
Specific toxicological data for 9-(2-Carboxy-2-cyanovinyl)julolidine are not extensively detailed. As a research chemical, it should be handled with standard laboratory precautions, avoiding inhalation, ingestion, and skin contact. It is not intended for human use.
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| References | |
| Additional Infomation |
9-(2-Carboxy-2-cyanovinyl)julolidine is a research-grade fluorescent probe for laboratory use only and is not approved for clinical use. Its CAS number is 142978-18-5. Its primary application is as a fluorescent molecular rotor for studying protein binding and microviscosity in biological and chemical systems. It is a valuable tool in biophysics and biochemistry.
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| Molecular Formula |
C16H16N2O2
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|---|---|
| Molecular Weight |
268.31044
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| Exact Mass |
268.121
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| CAS # |
142978-18-5
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| PubChem CID |
5353485
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| Appearance |
Orange to red solid powder
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| Density |
1.32g/cm3
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| Boiling Point |
539.4ºC at 760 mmHg
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| Flash Point |
280ºC
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| Vapour Pressure |
1.81E-12mmHg at 25°C
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| Index of Refraction |
1.65
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| LogP |
2.441
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
20
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| Complexity |
456
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CC2=CC(=CC3=C2N(C1)CCC3)/C=C(\C#N)/C(=O)O
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| InChi Key |
JXENNHTVELFRHV-NTEUORMPSA-N
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| InChi Code |
InChI=1S/C16H16N2O2/c17-10-14(16(19)20)9-11-7-12-3-1-5-18-6-2-4-13(8-11)15(12)18/h7-9H,1-6H2,(H,19,20)/b14-9+
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| Chemical Name |
(E)-3-(1-azatricyclo[7.3.1.05,13]trideca-5,7,9(13)-trien-7-yl)-2-cyanoprop-2-enoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7270 mL | 18.6352 mL | 37.2703 mL | |
| 5 mM | 0.7454 mL | 3.7270 mL | 7.4541 mL | |
| 10 mM | 0.3727 mL | 1.8635 mL | 3.7270 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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